Flash floods are often responsible for deaths and damage to infrastructure. The objective of this work is to create a data-driven model to understand how predisposing factors influence the spatial variation of the triggering factor (rainfall intensity) in the case of flash floods in the continental area of Portugal. Flash floods occurrences were extracted from the DISASTER database. We extracted the accumulated precipitation from the Copernicus database by considering two days of duration. The analysed predisposing factors for flooding were extracted considering the whole basin where each occurrence is located. These factors include the basin area, the predominant lithology, drainage density, and the mean or median values of elevation, slope, stream power index (SPI), topographic wetness index (TWI), roughness, and four soil properties. The Random Forest algorithm was used to build the models and obtained mean absolute percentage error (MAPE) around 19%, an acceptable value for the objectives of the work. The median of SPI, mean elevation and the area of the basin are the top three most relevant predisposing factors interpreted by the model for defining the rainfall input for flash flooding in mainland Portugal.
Accurately modeling soil-fluid coupling under large deformations is critical for understanding and predicting phenomena such as slope failures, embankment collapses, and other geotechnical hazards. This topic has been studied for decades and remains challenging due to the nonlinear responses of geotechnical structures, which typically result from plastic yielding and finite deformation of the soil skeleton. In this work, we comprehensively summarize the theory involved in the soil-fluid coupling problem. Within a finite strain framework, we employ an elasto-plastic constitutive model with linear hardening to represent the solid skeleton and a nearly incompressible model for water. The water content influences the behavior of the solid skeleton by affecting its cohesion. The governing equations are discretized by material point method and two sets of material points are employed to independently represent solid skeleton and fluid, respectively. The proposed method is validated by comparing simulation results with experimental results for the impact of water on dry soil and wet soil. The capability of the method is further demonstrated through two cases: (1) the impact of a rigid body on saturated soil, causing water seepage, and (2) the filling of a ditch, which considers the erosion of the foundation. This work may provide a versatile tool for analyzing the dynamic responses of fluid and solid interactions, considering both mixing and separation phenomena.
Researchers have tried hard to study the toxic effects of single pollutants like certain antibiotics and nanoplastic particles on plants. But we still know little about how these pollutants interact when they're together in the environment, and what combined toxic effects they have on plants. This study assessed the toxic effects of polystyrene nanoplastics (PS-NPs) and ciprofloxacin (CIP), both individually and in combination, on soybean (Glycine max L.) seedlings by various concentration gradients treatments of PS-NPs (0, 10, 100 mg/L) and CIP (0, 10 mg/L). The results indicated that high concentrations of PS-NPs significantly impeded soybean seedling growth, as evidenced by reductions in root length, plant height, and leaf area. CIP predominantly affected the physiological functions of leaves, resulting in a decrease in chlorophyll content. The combined exposure demonstrated synergistic effects, further intensifying the adverse impacts on the growth and physiological functions of soybean seedlings. Metabolomic analyses indicated that single and combined exposures markedly altered the metabolite expression profiles in soybean leaves, particularly related to amino acid and antioxidant defense metabolic pathways. These results indicate the comprehensive effects of NPs with antibiotics on plants and provide novel insights into toxic mechanisms.
Nanoplastics (NPs) and zinc (Zn), both widespread in soil environments, present considerable risks to soil biota. While NPs persist environmentally and act as vectors for heavy metals like Zn, their combined toxicity, especially in soil invertebrates, remains poorly understood. This study evaluates the individual and combined effects of Zn and NPs on earthworm coelomocytes and explores their interactions with Cu/Zn-superoxide dismutase (SOD), an antioxidant enzyme. Molecular docking revealed that NPs bind near the active site of SOD through pi-cation interactions with lysine residues, further stabilized by neighboring hydrophobic amino acids. Viability assays indicated that NPs alone (20 mg/L) had negligible impact (94.54 %, p > 0.05), Zn alone (300 mg/L) reduced viability to 80.02 %, while co-exposure reduced it further to 73.16 %. Elevated levels of reactive oxygen species (ROS) and malondialdehyde (MDA) levels were elevated to 186 % and 173 % under co-exposure, alongside greater antioxidant enzyme disruption, point to synergistic toxicity. Dynamic light scattering and zeta potential (From -13 to -7 mV) analyses revealed larger particle sizes in the combined system, indicative of enhanced protein interactions. Conformational changes in SOD, such as alpha-helix loss and altered fluorescence, further support structural disruption. These findings demonstrate that co-exposure to NPs and Zn intensifies cellular and protein-level toxicity via integrated physical and biochemical mechanisms, providing critical insight into the ecological risks posed by such co-contaminants in soil environments.
Seismic risk assessment of code-noncompliant reinforced concrete (RC) frames faces significant challenges due to structural heterogeneity and the complex interplay of site-specific hazard conditions. This study aims to introduce a novel framework that integrates three key concepts specifically targeting these challenges. Central to the methodology are fragility fuses, which employ a triplet of curves-lower bound, median, and upper bound-to rigorously quantify within-class variability in seismic performance, offering a more nuanced representation of code-noncompliant building behavior compared to conventional single-curve approaches. Complementing this, spectrum-consistent transformations dynamically adjust fragility curves to account for regional spectral shapes and soil categories, ensuring site-specific accuracy by reconciling hazard intensity with local geotechnical conditions. Further enhancing precision, the framework adopts a nonlinear hazard model that captures the curvature of hazard curves in log-log space, overcoming the oversimplifications of linear approximations and significantly improving risk estimates for rare, high-intensity events. Applied to four RC frame typologies (2-5 stories) with diverse geometries and material properties, the framework demonstrates a 15-40 % reduction in risk estimation errors through nonlinear hazard modeling, while spectrum-consistent adjustments show up to 30 % variability in exceedance probabilities across soil classes. Fragility fuses further highlight the impact of structural heterogeneity, with older, non-ductile frames exhibiting 25 % wider confidence intervals in performance. Finally, risk maps are presented for the four frame typologies, making use of non-linear hazard curves and spectrumconsistent fragility fuses accounting for both local effects and within-typology variability.
Constitutive models of sands play an essential role in analysing the foundation responses to cyclic loads, such as seismic, traffic and wave loads. In general, sands exhibit distinctly different mechanical behaviours under monotonic, regular and irregular cyclic loads. To describe these complex mechanical behaviours of sands, it is necessary to establish appropriate constitutive models. This study first analyses the features of hysteretic stressstrain relation of sands in some detail. It is found that there exists a largest hysteretic loop when sands are sufficiently sheared in two opposite directions, and the shear stiffness at a stress-reversal point primarily depends on the degree of stiffness degradation in the last loading or unloading process. Secondly, a stress-reversal method is proposed to effectively reproduce these features. This method provides a new formulation of the hysteretic stress-strain curves, and employs a newly defined scalar quantity, called the small strain stiffness factor, to determine the shear stiffness at an arbitrary stress-reversal state. Thirdly, within the frameworks of elastoplastic theory and the critical state soil mechanics, an elastoplastic stress-reversal surface model is developed for sands. For a monotonic loading process, a double-parameter hardening rule is proposed to account for the coupled compression-shear hardening mechanism. For a cyclic loading process, a new kinematic hardening rule of the loading surface is elaborately designed in stress space, which can be conveniently incorporated with the stressreversal method. Finally, the stress-reversal surface model is used to simulate some laboratory triaxial tests on two sands, including monotonic loading tests along conventional and special stress paths, as well as drained cyclic tests with regular and irregular shearing amplitudes. A more systematic comparison between the model simulations and relevant test data validates the rationality and capability of the model, demonstrating its distinctive performance under irregular cyclic loading condition.
This study was carried out to evaluate the interaction between terrestrial food crop plants and microplastics (MPs) with a focus on understanding their uptake, effects on growth, physiological, biochemical, and yield characteristics of two different cultivars of Solanum tuberosum L. i.e., Variety-1, Astrix (AL-4) and Variety-2, Harmes (WA-4). Polyethylene (PE), polystyrene (PS), and polypropylene (PP) spheres of size 5 mu m were applied to the soil at concentrations of 0 %, 1 %, and 5 %. Morphological parameters, including seed germination rate, shoot and root lengths, leaf area, and fresh and dry biomass of plants, got reduced significantly with the increase in MP concentration. PS MPs caused the most negative impact, particularly at 5 %, leading to the greatest decline in growth and Na, Mg, Zn, Cu, Ni, and Mn nutrient content. The highest DPPH scavenging activity was observed in the 5 % PS MPs treatment with approximately a 45.34 % increase from the control, indicating its potential to enhance antioxidant activity in response to stress caused by PS MPs. Both reducing and non-reducing sugar contents and total proteins were also decreased significantly. Vitamin C content exhibited a significant increase in response to MPs, with the highest levels recorded under 5 % PS MPs treatments. This suggests an adaptive antioxidant response to mitigate oxidative damage induced by MPs. SEM analysis revealed tissue infiltration of MP particles in shoots, leaves, and tubers of both varieties. Among MPs, PS had the most detrimental effects, followed by PP and PE, with higher concentrations increasing the negative impact.
Drought and salt stress are two major abiotic factors significantly impacting crop growth and yield. Climate change leads to increasing drought and soil salinization issues, rising significant challenges to agricultural production. Amylases play a crucial role in enhancing the tolerance of crops to these stresses by regulating physiological and enzymatic activities. Previous study identified MeAMY1 and MeBAM3 as key genes involved in cassava starch metabolism under drought stress. To investigate their functions under drought and salt stress, MeAMY1 and MeBAM3 genes were cloned and over-expressed in Arabidopsis thaliana in the current study. Overexpression of MeAMY1 in Arabidopsis enhances amylase activities, promotes starch hydrolysis, releases soluble sugar and thus enhances osmotic balance in transgenic Arabidopsis. In the mean while, expression of BAM1 and SEX1 were depressed by MeAMY1 to maintain the protects cells closed under stress and preserved starch for adapting the stressful environments. Overexpression of the MeBAM3 in Arabidopsis can increase the expression levels of AMY3 and RVE1, promotes starch hydrolysis, releases soluble sugar from the chloroplasts to the cytoplasm and thus enhances osmoregulatory substance content, reducing stress-induced damage to antioxidant enzymes and cell membranes and improving stress tolerance. The principal component analysis further indicated that MeAMY1 and MeBAM3 overexpression lines responded similarly to drought stress, while MeBAM3 overexpression provided greater resilience to salt stress.
This study explores the effectiveness of soft viscoelastic biopolymer inclusions in mitigating cyclic liquefaction in loosely packed sands. This examination employs cyclic direct simple shear testing (CDSS) on loose sand treated with gelatin while varying the gelatin concentration and the cyclic stress ratio (CSR). The test results reveal that the inclusion of soft, viscoelastic gelatin significantly reduces shear strain and excess pore pressure during cyclic shear. Liquefaction potential, defined as the number of cycles to liquefaction (NL) at an excess pore pressure ratio (ru = Delta u/sigma ' vo) of 0.7, is substantially improved in gelatin-treated sands compared to gelatin-free sands. This improvement in liquefaction resistance is more pronounced as the inclusion stiffness increases. Furthermore, the viscoelastic pore-filling inclusion helps maintain skeletal stiffness during cyclic shearing, resulting in a higher shear modulus in gelatin-treated sand in both small and large-strain regimes. At a grain scale, pore-filling viscoelastic biopolymers provide structural support to the skeletal frame of a loosely packed sand. This pore filler mitigates volume contraction and helps maintain the effective stress of the soil structure, thereby reducing liquefaction potential under cyclic shearing. These findings underscore the potential of viscoelastic biopolymers as bio-grout agents to reduce liquefaction risk in loose sands.
The mitigation of seismic soil liquefaction in sand with fine content presents a challenge, demanding efficient strategies. This research explores the efficacy of Microbial-Induced Partial Saturation (MIPS) as a biogeotechnical technique to improve the liquefaction resistance of sandy soils with plastic fines. By leveraging the natural metabolic processes of indigenous microorganisms, this method introduces biogenic gas production within the soil matrix, effectively reducing its degree of saturation. This partial desaturation alters the soil's response to cyclic loading, aiming to mitigate the risk of liquefaction under dynamic loading conditions. Experimental results from a series of undrained strain-controlled cyclic shear tests reveal that even a modest reduction in saturation significantly enhances the soil's stability against seismic-induced liquefaction. The investigation extends to analyzing the effectiveness of the MIPS treatment in sands with low-plasticity clay content, offering insights into the interaction between microbial activity, soil texture, and liquefaction potential. Results show that while plasticity plays a key role in improving the cyclic response of soils, the influence of MIPS treatment remains noteworthy, even in sand with plastic fines. Additionally, a modified predictive formulation is introduced, incorporating a calibrated parameter to account for the influence of fines' plasticity on excess pore pressure generation.