The use of mixed forests and non-native tree species have the potential to mitigate climate change impacts and enhance biodiversity. However, little is known about how forest composition and environmental factors affect each step of natural regeneration in mixed forests, especially in mixtures with non-native trees. Here we investigated how forest composition affected European beech seed survival (through seed tracking), seed sprouting (via field germination experiments), and seedling survival (monthly seedling monitoring) in pure beech forests and in admixtures with Norway spruce and the introduced Douglas-fir in a mast and non-mast year of beech. We also assessed how biotic and abiotic factors (small mammal abundance, ungulate access, seed production, seed burial, canopy cover, distance to nearest adult tree, seedling aggregation, understory density, browsing damage, and soil properties) affected these regeneration dynamics. Seed survival was negatively affected by the presence of conifers and mouse abundance. Seed germination was influenced by whether seeds were buried or not. Seedling survival increased in Douglas-fir admixtures, and in forests with higher soil quality. Browsing damage and ungulate access diminished seedling survival. Seed production had the greatest influence on absolute number of seedlings. Forest composition and environmental factors had distinct impacts on regeneration of beech depending on its ontogenetic stage. Here, we provide evidence supporting the notion that Douglas-fir is not impairing the regeneration of native trees in mixed forests. In fact, mixtures with Douglas-fir benefited the survival of beech seedlings, likely due to better soil properties and less damage from herbivores on these stands.

For establishment and growth of newly planted seedlings it is essential to overcome environmental stress at the planting site. Adding the amino acid arginine at planting is a novel treatment aiming at increased establishment success, so far tested in a limited number of applied studies. We examined the effects of adding arginine-phosphate (arGrow (R)), mechanical site preparation (MSP), and planting time on survival and growth of Norway spruce and Scots pine seedlings in two field experiments in boreal southeastern Norway. After three growing seasons, survival for spring planted seedlings of both species was significantly better following MSP, while addition of arginine-phosphate did not have any effect. Autumn planted pine seedlings with MSP and arginine had higher survival and also larger diameter than spring planted ones with MSP but without arginine. Spruce and pine seedlings with MSP were taller and had larger diameter than those without MSP. For spring planted seedlings of both species, dry weight of roots and shoots was positively affected by MSP, but not by arginine. To conclude, arginine-phosphate had neutral to modestly positive effects on survival and growth, while MSP had clear positive effects. The effect of planting time varied with species.

The forest regeneration phase in Sweden commonly involves mechanical soil preparation followed by the planting of Scots pine (Pinus sylvestris L.) or Norway spruce (Picea abies (L.) Karst) seedlings. The prepared soil offers planting positions with different properties, including reduced damage by pine weevils (Hylobius abietis L.). Nitrogen fertilization can be applied at the time of planting to aid establishment of the seedlings. In this study, we compared the effects of different planting positions, organic nitrogen fertilization, and different seedling sizes on the early survival and growth of Scots pine and Norway spruce seedlings. The main planting positions were capped mound, hinge, and mineral soil. Seedlings planted close to organic material were categorized as being in low-quality positions, since proximity to organic material increases pine weevil attraction. Higher mortality rates related to pine weevil damage were recorded for the seedlings planted in the low-quality positions, regardless of seedling size or N fertilization. Pine weevil attack rates increased with increasing seedling size. Growth was, in general, lowest in the mineral soil positions. The effect of organic N fertilization on growth was positive for the spruce regardless of the planting position or seedling size, while it depended on the planting position and seedling size for the pine, indicating that the effects of organic N fertilization depend on the seedling species, seedling size, and planting position.