For establishment and growth of newly planted seedlings it is essential to overcome environmental stress at the planting site. Adding the amino acid arginine at planting is a novel treatment aiming at increased establishment success, so far tested in a limited number of applied studies. We examined the effects of adding arginine-phosphate (arGrow (R)), mechanical site preparation (MSP), and planting time on survival and growth of Norway spruce and Scots pine seedlings in two field experiments in boreal southeastern Norway. After three growing seasons, survival for spring planted seedlings of both species was significantly better following MSP, while addition of arginine-phosphate did not have any effect. Autumn planted pine seedlings with MSP and arginine had higher survival and also larger diameter than spring planted ones with MSP but without arginine. Spruce and pine seedlings with MSP were taller and had larger diameter than those without MSP. For spring planted seedlings of both species, dry weight of roots and shoots was positively affected by MSP, but not by arginine. To conclude, arginine-phosphate had neutral to modestly positive effects on survival and growth, while MSP had clear positive effects. The effect of planting time varied with species.