Salt-affected soils severely decrease agricultural productivity by reducing the uptake of water and nutrients by plants, toxic ions accumulation and soil structure degradation. The sustainable synthesis of hybrid nanospheres through green approaches has emerged as an effective strategy to enhance crop productivity and improve tolerance to abiotic stress. However, the defensive functions and fundamental mechanisms of green synthesized calcium-doped carbon nano-spheres in protecting maize against salt stress remain elusive. Thus, calcium-doped carbon nanospheres were innovatively synthesized by doping calcium oxide nanoparticles (CaO NPs) with lignin nanoparticles (LNPs) which were further analyzed using Fourier Transform Infrared Spectroscopy (FT-IR), Energy Dispersive X-Ray Spectroscopy (EDX), Field Emission Scanning Electron Microscopy (FE-SEM) and Transmission Electron Microscopy (TEM). These analyses validated the successful doping of Ca@CNs, elucidating the purity and morphology of the hybrid nanospheres. More importantly, the effect of Ca@CNs on maize plants under NaCl stress, unreported so far, was examined. Results of the current study showed that treating salt-stressed plants with Ca@CNs significantly improved maize growth and biomass accumulation by enhanced absorption of minerals and improved photosynthetic efficiency. Furthermore, Ca@CNs application has also reduced NaCl-induced oxidative damage by enhancing antioxidant defense mechanisms and maintaining cellular integrity, resulting in improved resistance to salt stress. Moreover, Ca@CNs substantially up-regulated the expression of salt-tolerant genes ZmNHX3, CBL, ZmHKT1, and MAPK1, as well as genes involved in lignin biosynthesis such as 4CL2, PAL1, CCR, and COMT, in both shoot and root tissues. Conversely, the expression levels of genes Zm00001d003114, Zm0001d026638, Zm00001d028582 and Zm00001d051069 associated with Ca2 +-responsive SOS3 pathway were all down-regulated under NaCl treatment, while up-regulated in the presence of Ca@CNs along with NaCl. The observed changes in transcript levels of these genes highlight the potential of Ca@CNs in alleviating NaCl toxicity. These results demonstrated that the green synthetic Ca@CNs can significantly alleviate salt stress and promote plant growth in saline environments, which will provide a new strategy for the utilization of nanoparticles in agriculture to maintain sustainable agriculture and improve crop yield.

Soil salinization is increasingly recognized as a critical environmental challenge that significantly threatens plant survival and agricultural productivity. To elucidate the mechanism of salt resistance in poplar, physiological and transcriptomic analyses were conducted on 84K poplar (Populus alba x Populus glandulosa) under varying salt concentrations (0, 100, 200 and 300 mM NaCl). As salt levels increased, observable damage to poplar progressively intensified. Differentially expressed genes under salt stress were primarily enriched in photosynthesis, redox activity and glutathione metabolism pathways. Salt stress reduced chlorophyll content and net photosynthetic rate, accompanied by the downregulation of photosynthesis-related genes. NaCl (300 mM) significantly inhibited the photochemical activity of photosystems. The higher photochemical activity under 100 and 200 mM NaCl was attributed to the activated PGR5-cyclic electron flow photoprotective mechanism. However, the NAD(P)H dehydrogenase-like (NDH)-cyclic electron flow was inhibited under all salt levels. Salt stress led to reactive oxygen species accumulation, activating the ASA-GSH cycle and antioxidant enzymes to mitigate oxidative damage. Weighted gene co-expression network analysis showed that five photosynthesis-related hub genes (e.g., FNR and TPI) were down-regulated and nine antioxidant-related hub genes (e.g., GRX, GPX and GST) were up-regulated under salt stress conditions. PagGRXC9 encodes glutaredoxin and was found to be differentially expressed during the salt stress condition. Functional studies showed that overexpressing PagGRXC9 enhanced salt tolerance in yeast, and in poplar, it improved growth, FV/FM, non-photochemical quenching values and resistance to H2O2-induced oxidative stress under salt stress. This study constructed the photosynthetic and antioxidant response network for salt stress in poplar, revealing that PagGRXC9 enhances salt tolerance by reducing photoinhibition and increasing antioxidant capacity. These findings provide valuable insights for breeding salt-tolerant forest trees.

Salinity stress (NaCl) and heavy metals contamination (CdCl2) are the serious environmental constraints for decreased crop production worldwide. However, the interaction between NaCl and CdCl2 regarding sodium (Na), cadmium (Cd), and chloride (Cl) accumulation in plants has not been completely established. Therefore, the interactive effects of NaCl andCdCl2 on plant growth, Na, Cd, and Cl accumulation in plants, and wheat yield were evaluated. Wheat seeds were cultivated in clay loam soil under greenhouse conditions. After two weeks of sowing, plants were subjected to NaCl at the rate of 0, 50, and 100 mM either alone or in combination with CdCl2: 0, 1, and 2 mM, respectively. The results revealed that increasing NaCl and CdCl2 levels reduced Na and Cd concentrations, whereas enhanced Cl concentrations. Furthermore, moderate levels of CdCl2 and NaCl stresses enhanced the antioxidative enzymatic activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in addition to proline accumulation in wheat leaves. By contrast, 100 mM NaCl in combination with 2 mM CdCl2 enhanced H2O2 accumulation by 105%, which thus decreased the membrane stability index (MSI) by 49% and wheat yield by 27% as compared to 2 mM CdCl2. The reduced Cd toxicity by NaCl or Na accumulation in plant tissues by CdCl2 involved competition between Na and Cd at binding sites, however, enhanced Cl phytotoxicity in plants resulted in the overproduction of H2O2 that was not quenched by antioxidative enzymes, thereby decreased MSI and wheat yield.

Selenium (Se) plays a crucial role in ameliorating the negative impact of abiotic stress. The present study was performed to elucidate the efficacy of soil treatment of Se in reducing salt-induced stress in Carthamus tinctorius L. In this study, three different levels of Na2SeO4 (0, 0.01, and 0.02 g kg- 1) and four levels of NaCl (0, 0.5, 1.5, and 2.5 g kg- 1) were applied. The findings revealed that while NaCl decreased seed germination parameters, growth characteristics, K+ content, relative water content (RWC), and photosynthetic pigments, it increased Na+ content, soluble carbohydrates, H2O2 content, and malondialdehyde (MDA) level. The application of Se showed a positive effect on seed germination and growth characteristics under salinity conditions, which is linked to alterations in anatomical, biochemical, and physiological factors. Anatomical studies showed that treatment with Se led to increased stem diameter, cortical parenchyma thickness, and pith diameter under salinity stress. However, variations in the thickness of the xylem and phloem did not reach statistical significance. The application of Se (0.02 g kg- 1) raised Na+ content (7.65%), K+ content (29.24%), RWC (15%), Chl a (17%), Chl b (21.73%), Chl a + b (16.9%), Car (4.22%), and soluble carbohydrates (11%) in plants subjected to NaCl (2.5 g kg- 1) stress. Furthermore, it decreased H2O2 (25.65%) and MDA (11.9%) in the shoots. The findings of the current study advocate the application of the Se-soil treating technique as an approach for salt stress mitigation in crops grown in saline conditions.

There is a significant variability of salinity level in sensitive marine clays (SMC), which will produce an important impact on the development of mechanical characteristics in stabilized SMC. The influences of salt content (NaCl salt: 3, 10, and 20 g/L) on mechanical properties evolution of cement-stabilized SMC under different curing time (1, 7, 28, 60, and 90 days) have been experimentally investigated and modeled. The results indicate that the strength and modulus increase constantly with time but the time rates decrease. Meanwhile, the apparent improvement of strength and modulus at early age (up to 7 days) is observed. Higher NaCl content can bring a larger strength gain to stabilized SMC after same curing time and the enhancing effect of high salt contents (10 and 20 g/L) becomes more obvious with the extension of curing time. Whereas, the enhancing effect of high NaCl content on modulus is limited compared with strength. Further improvement provided by excessive NaCl salt (20 g/L) is not as effective. In addition, the predictive models have been established to quantitatively evaluate the evolution of mechanical properties in stabilized SMC with different NaCl contents. The capability of developed models has been demonstrated through the good agreement between simulated and experimental results.

Backround The utilization of high-quality water in agriculture is increasingly constrained by climate change, affecting availability, quality, and distribution due to altered precipitation patterns, increased evaporation, extreme weather events, and rising salinity levels. Salinity significantly challenges salt-sensitive vegetables like lettuce, particularly in a greenhouse. Hydroponics water quality ensures nutrient solution stability, enhances nutrient uptake, prevents contamination, regulates pH and electrical conductivity, and maintains system components. This study aimed to mitigate salt-induced damage in lettuce grown via the floating culture method under 50 mM NaCl salinity by applying biostimulants. Results We examined lettuce's physiological, biochemical, and agronomical responses to salt stress after applying biostimulants such as amino acids, arbuscular mycorrhizal fungi, plant growth-promoting rhizobacteria (PGPR), fulvic acid, and chitosan. The experiment was conducted in a greenhouse with a randomized complete block design, and each treatment was replicated four times. Biostimulant applications alleviated salt's detrimental effects on plant weight, height, leaf number, and leaf area. Yield increases under 50 mM NaCl were 75%, 51%, 31%, 34%, and 33% using vermicompost, PGPR, fulvic acid, amino acid, and chitosan, respectively. Biostimulants improved stomatal conductance (58-189%), chlorophyll content (4-10%), nutrient uptake (15-109%), and water status (9-107%). They also reduced MDA content by 26-42%. PGPR (1.0 ml L-1), vermicompost (2 ml L-1), and fulvic acid (40 mg L-1) were particularly effective, enhancing growth, yield, phenol, and mineral content while reducing nitrate levels under saline conditions. ConclusionsBiostimulants activated antioxidative defense systems, offering a sustainable, cost-effective solution for mitigating salt stress in hydroponic lettuce cultivation.

Generally, artificial ground freezing (AGF) technology is utilized to guarantee tunnel safety during construction. However, the soil structure changes significantly after freeze-thaw, resulting in uneven deformation of the tunnel under traffic loading from subway vibration. To solve this problem effectively, it is necessary to consider the combined impact of freeze-thaw, salt, and traffic loading damage that marine soft soil must withstand simultaneously. For this reason, cyclic triaxial test and NMR test were performed on the silty clay saturated with NaCl solution in this study. The influence of three main factors on dynamic properties has been thoroughly investigated, namely freeze-thaw, salt content, and confining pressure. According to cyclic triaxial test, the shape of the hysteresis loop of the specimens after freeze-thaw changed more significantly with increasing loading cycles. The dynamic elastic modulus was weakened by freeze-thaw, while improved by the addition of NaCl. Damping ratio was consistent with the dynamic elastic modulus law. It was worth noting that the different freezing temperatures (-10 degrees C, -20 degrees C and - 30 degrees C) had only a slight impact on dynamic elastic modulus, as well as damping ratio. Mathematical models were proposed to forecast the dynamic elastic modulus and damping ratio regarding marine soft clay. NMR test indicated that the addition of salt made the internal pore environment of the specimens tend to be consistent and enhanced the water-solid interaction. The increase in porosity resulted in the decrease in dynamic elastic modulus. The results have provided valuable insights into the mechanical characteristics of marine soft clay when AGF technology is applied.

Graphene is regarded as a promising additive to enhance the thermal conductivity of bentonite in the geological repository, yet the hydro-mechanical properties of its mixture with bentonite under chemical conditions remain unclear. This work examined the one-dimensional swelling deformation of high-density graphene-modified bentonite (GMB) in NaCl solutions, considering the influences of graphene content and NaCl concentration. Results indicate that both graphene and NaCl reduced the maximum swelling strain and the primary and secondary swelling coefficients of GMB. Additionally, graphene enhanced the stability of swelling deformation in bentonite across various NaCl concentrations and decreased the GMB's test duration. The relationship between test duration and NaCl concentration was nonmonotonic. The ratio of swelling strain at each stage to total swelling strain was rarely affected by graphene content or NaCl solution. Scanning electron microscope examinations on selected samples post-swelling tests unveiled a unique soil structure in GMB. Furthermore, a model was put forward to predict the maximum swelling strain of GMB inundated in NaCl solutions, and its feasibility was verified.

Artificial ground freezing (AGF) technology is sometimes adopted to reinforce the surrounding soil to ensure the safety of subway tunnels during construction in the eastern coastal areas of China. The mechanical characteristics of frozen soil are subjected to change due to the incorporation of chlorine salt, and the soil strength decreases significantly with an increase of salt content. The mechanical properties of frozen silty clay have been rarely investigated due to the coupling effect of temperature, strain rate, and salt content. In this study, before the application of AGF, a series of unconfined compressive strength (UCS) tests and the consolidated-undrained static triaxial compression tests were conducted on frozen silty clay to investigate the influence of salt content, temperature, and strain rate on the mechanical properties of frozen soil. From the stress -strain curves of the UCS test, strain -hardening and strain -softening behavior were identified as a result of the influence of different temperatures and salt contents. Regression analysis was performed to discriminate the effect of each individual factor and their coupling effect. The results reveal that soil UCS logarithmically increased with a decrease in relative temperature and strain rate. Both UCS and static triaxial strength linearly decreased with an increase in salt content. The influence of salt content on the strength of frozen soil was discussed and compared with previous researches. Furthermore, an elastic -plastic constitutive model was proposed to characterize the stress -strain behavior in which the effects of strain rate and salt content were included. At last, with consideration of salt content, a modified constitutive model was proposed to describe the strength and deformation behavior of frozen silty clay.

The excess of salts in soils causes stress in most plants, except for some halophytes that can tolerate higher levels of salinity. The excess of Na + generates an ionic imbalance, reducing the K + content and altering cellular metabolism, thus impacting in plant growth and development. Additionally, salinity in soil induces water stress due to osmotic effects and increments the production of reactive oxygen species (ROS) that affect the cellular structure, damaging membranes and proteins, and altering the electrochemical potential of H + , which directly affects nutrient absorption by membrane transporters. However, plants possess mechanisms to overcome the toxicity of the sodium ions, such as internalization into the vacuole or exclusion from the cell, synthesis of enzymes or protective compounds against ROS, and the synthesis of metabolites that help to regulate the osmotic potential of plants. Physiologic and molecular mechanisms of salinity tolerance in plants will be addressed in this review. Furthermore, a revision of strategies taken by researchers to confer salt stress tolerance on agriculturally important species are discussed. These strategies include conventional breeding and genetic engineering as transgenesis and genome editing by CRISPR/Cas9.