Using Aloe Vera powder (AV) at varying concentrations - 1, 2, and 3% - polylactic acid/aloe vera (PLA/AV) composite films were prepared using the solvent casting process. All of the composites were exposed to 10, 25, and 40 kGy of electron beam (EB) radiation. It was examined how the thermal and mechanical characteristics of PLA/AV films were affected by electron beam radiation. XRD, FTIR, TGA, and biodegradation (soil burial) were used to analyze the irradiation films' characteristics. The findings showed that doses up to 25 kGy increased the neat PLA's tensile strength (TS). At lower doses up to 10 kGy, the addition of AV raises the TS values (particularly at 2% concentration). It appears adding varying proportions of AV powder enhances the thermal stability of PLA/AV composites. Biodegradability showed that films with AV were the most biodegradable, while those without AV were the least.