Drought may impact plant-soil biotic interactions in ways that modify aboveground herbivore performance, but the outcomes of such biotic interactions under future climate are not yet clear. We performed a growth chamber experiment to assess how long-term, drought-driven changes in belowground communities influence plant growth and herbivore performance using a plant-soil feedback experimental framework. We focussed on two common pasture legumes-lucerne, Medicago sativa L., and white clover, Trifolium repens L. (both Fabaceae)-and foliar herbivores-cotton bollworm, Helicoverpa armigera (H & uuml;bner) (Lepidoptera: Noctuidae), and two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Soil was collected from a field facility where rainfall had been manipulated for 6 years, focussing on treatments representing ambient rainfall and prolonged drought (50% reduction relative to ambient), to consider the effects of biological legacies mediated by the prolonged drought. All soils were sterilized and re-inoculated to establish the respective home (i.e. where a given plant is cultivated in its own soil) and away (i.e. where a given plant is cultivated in another species' soil) treatments in addition to a sterile control. We found that the relative growth rate (RGR) and relative consumption of larvae were significantly lower on lucerne grown in soil with ambient rainfall legacies conditioned by white clover. Conversely, the RGR of insect larvae was lower on white clover grown in soil with prolonged drought legacies conditioned by lucerne. Two-spotted spider mite populations and area damage (mm2) were significantly reduced on white clover grown in lucerne-conditioned soil in drought legacies. The higher number of nodules found on white clover in lucerne-conditioned soil suggests that root-rhizobia associations may have reduced foliar herbivore performance. Our study provides evidence that foliar herbivores are affected by plant-soil biotic interactions and that prolonged drought may influence aboveground-belowground linkages with potential broader ecosystem impacts.

Fire has important effects on soil properties and functioning in terrestrial ecosystems that have been explored by many studies. Limited information exists on the alterations in soil parameters over time caused by fire disturbance in semi-arid climates. This study is designed to examine the influence of fire disturbance on the change of soil physical, chemical, and biological properties over time in a semi-arid region. In the summer of 2007, a severe natural fire occurred in the Pideh region of northern Iran, dominated by hawthorn (Crataegus melanocarpa M.B.) and berberis (Berberis integerrima Bunge), which destroyed almost 80 % of the shrubs and the majority of the co-dominant plants over a vast area. For this research, 12 soil samples (0-10 cm depth) were taken in summer (August) in different years (i.e., 2010, 2013, 2016, 2019, and 2022) from the burnt area. Furthermore, a total of 12 soil samples were collected during the summer (August) of 2022 from unburned regions to serve as a control. Soil biological parameters were studied by conducting soil samplings in the summer (August) and autumn (November) of every year. To evaluate soil N mineralization, soil samplings were done in summer (August and September) and autumn (November and December). Our results indicated that the occurrence of fire increased soil bulk density, with a concomitant decline in soil organic matter (SOM), porosity, aggregate stability, particulate organic carbon and nitrogen (POC and PON), as well as available nutrients such as ammonium (NH4+) and nitrate (NO3-) levels. Additionally, microbial parameters (respiration and biomass) and enzymes (urease, acid phosphatase, arylsulfatase and invertase), experienced a decrease in areas affected by the fire over time of 2010 to 2022. Unburnt (2022) and burnt (2022) sites had higher density and biomass of the three earthworm groups. Acari, Collembola, nematodes, protozoans, fungi and bacteria were significantly affected by fire disturbance in the different seasons, and years, and declined in the order unburnt sites > burnt sites 2022 > burnt sites 2019 > burnt sites 2016 > burnt sites 2013 > burnt sites 2010, respectively. Fire has complex effects on soil, involving interactions among physical, chemical, and biological properties that may persist for a prolonged period. After fifteen years of fire disturbance, soil characteristics were different in the burned (2022) and unburned areas. This research offers valuable insights into the impact of fire on soil characteristics over time, as well as a comparison with an unburned area. Therefore, it is essential to adopt soil management practices to minimize soil disruption in burned areas and facilitate the full recovery of soil ecosystems after fire damage.

The Arctic soil communities play a vital role in stabilizing and decomposing soil carbon, which affects the global carbon cycling. Studying the food web structure is critical for understanding biotic interactions and the functioning of these ecosystems. Here, we studied the trophic relationships of (microscopic) soil biota of two different Arctic spots in Ny-angstrom lesund, Svalbard, within a natural soil moisture gradient by combining DNA analysis with stable isotopes as trophic tracers. The results of our study suggested that the soil moisture strongly influenced the diversity of soil biota, with the wetter soil, having a higher organic matter content, hosting a more diverse community. Based on a Bayesian mixing model, the community of wet soil formed a more complex food web, in which bacterivorous and detritivorous pathways were important in supplying carbon and energy to the upper trophic levels. In contrast, the drier soil showed a less diverse community, lower trophic complexity, with the green food web (via unicellular green algae and gatherer organisms) playing a more important role in channelling energy to higher trophic levels. These findings are important to better understand the soil communities inhabiting the Arctic, and for predicting how the ecosystem will respond to the forthcoming changes in precipitation regimes. Wetter soils, with a higher organic matter content, host more diverse soil biota and support more complex food webs, in which bacterivorous and detritivorous pathways are relevant in supplying energy.