PurposeThe study focused on developing a rapid PCR-based detection method and employing gamma irradiation techniques to manage Ralstonia solanacearum, aiming to produce brown rot-free export-quality potatoes. This initiative seeks to enhance potato exports from Bangladesh.Materials and MethodsSamples of potato tubers and soil were collected from various commercially significant potato-growing areas, resulting in a total of 168 Ralstonia solanacearum isolates from potato tubers and soil across 12 regions. The detection of R. solanacearum in the enriched tuber extract and soil were conducted using the primer pairs (PS-1, PS-2) and (759, 760). For the gamma irradiation experiment, petri dishes containing R. solanacearum cultures were subjected to different doses of gamma rays at the Bangladesh Institute of Nuclear Agriculture using a 60Co source. The irradiation doses applied to the samples were 0-6.0KGy.ResultsMorphological identification based on pink/light red colonies on TTC medium was confirmed R. solanacearum in 148 isolates. PCR using species-specific primers (PS-1/PS-2) and (759, 760) verified 26 isolates (14 tubers, 12 soil), producing 553 bp and 281 bp fragments in latently infected tubers and soil samples respectively. Gamma irradiation at 2.5 kGy damaged R. solanacearum's DNA and cells, preventing brown rot, while higher doses eliminated it entirely. This offers a promising strategy to enhance safety of stored potatoes, potentially mitigating economic losses from this quarantine pathogen.ConclusionThe study developed a PCR detection method and gamma irradiation techniques to manage R. solanacearum, enhancing the export quality of potatoes.

To investigate the influencing factors and intrinsic relationships between potato impact force and impact damage during potato soil separation, a testing system for potato impact force was established. The impact force test system is composed of a base, a height adjustment device, a simple separation screen, a soil storage tank, an impact force sensor, and so on. By allowing potatoes to fall freely to simulate the collision process, impact force data are collected, and a high-speed camera is used to locate the impact position and analyze the degree of damage. Through the response surface analysis method, the influencing factors and laws of the impact force and impact damage during the collision process between potatoes and rods under soil and no-soil conditions were studied. The results of the response surface analysis indicate that when the screen inclination is within the range of 14.12 degrees to 14.77 degrees, falling height ranges from 453.83 mm to 500 mm, screen rod spacing falls within 36.50 mm to 40 mm, and the screen rod material is rubber. Potatoes can still be at a relatively low damage level when enduring a large impact force. This study has significant implications for reducing potato impact damage during harvesting, enhancing economic benefits in the potato industry, and advancing the technical level of potato harvesting equipment. In the future, based on the results of this study, further exploration can be made to optimize the design of potato harvesting equipment so as to better reduce the damage to potatoes during harvesting and subsequent processing processes and promote the sustainable development of the potato industry.

The mechanized harvesting level of potatoes in the arid areas of Northwest China is low and mainly relies on simple machinery to dig the soil surface, and then people manually pick up and bag the potatoes. This harvesting method has the problems of a high labor intensity, low operation efficiency, and high labor cost. Based on this, a wheeled-chassis potato combine harvester with integrated bagging and ton bag-lifting systems was developed, which could complete potato digging, potato-soil separation, potato-film separation, automatic bagging, and field ton bag lifting in one go. Firstly, based on the agronomic requirements and unique terrain characteristics of potato planting in this area, the structural design of the whole machine was completed with SOLIDORKS 2019 3D software. Secondly, the dynamic model was established for a numerical analysis, and the core parameters of key components were determined. The field experiments showed that the potato loss rate was 2.1%, the potato damage rate was 1.7%, the skin breaking rate was 2.5%, the impurity content was 1.9%, and the productivity was 0.15 similar to 0.23 hm(2)/h. The above field test indexes met the requirements of national and industrial standards.