The development of a nonconventional nitrogen fertilizer, which can be fixed in agricultural fields using decentralized renewable energy sources, presents a feasible solution for sustainable on-site nitrogen fixation and fertilization. This study focuses on plasma-generated dinitrogen pentoxide (N2O5) as a prospective mediator for the on-site nitrogen fertilization, allowing nitrogen fertilization directly into culture media. Basal dinitrogen pentoxide fertilization demonstrated almost 100% dinitrogen pentoxide dissolution efficiency as nitrate in a culture medium and nitrogen fertilization effect on plant growth without explicit symptoms of damage. Top-dressing of dinitrogen pentoxide was also an efficient method for transferring nitrogen into the soil as nitrate, which improved plant growth and suppressed nitrogen deficiency symptoms, while overdose caused adverse effects. Plants can be grown with dinitrogen pentoxide (N2O5), acting as a nitrogen fertilizer remotely synthesized from air by on-site plasma nitrogen fixation. Both basal N2O5 fertilization and top-dressing of N2O5 gas over soil significantly improved plant growth owing to high dissolution efficiency into liquid and soil. image