Plants can sustain various degrees of damage or compensate for tissue loss by regrowth without significant fitness costs. This tolerance to insect herbivory depends on the plant's developmental stage during which the damage is inflicted and on how much tissue is removed. Plant fitness correlates, that is, biomass and germination of seeds, were determined at different ontogenetic stages, vegetative, budding, or flowering stages of three annual brassicaceous species exposed to feeding by Pieris brassicae caterpillars at different intensities. Fitness costs decreased with progressive ontogenetic stage at which damage was inflicted. Feeding on meristem tissues on vegetative and budding plants limited the plant's ability to fully compensate for tissue loss, whereas feeding on flowers resulted in full compensation or overcompensation in Sinapis arvensis and Brassica nigra. Herbivory promoted germination of seeds in the following year, thereby causing a shift in relative contribution to the next year's generation at the expense of contributing to the long-lived seed bank. Herbivory intensity affected fitness correlates of B. nigra and to a lesser extent of Sisymbrium officinale, but not of S. arvensis, demonstrating that even closely related plant species can differ in their specific responses to herbivory and that these can differently affect reproductive output. In terms of fitness costs, annual plant species can be quite resilient to herbivory. However, the extent to which they tolerate tissue loss depends on the ontogenetic stage that is under attack. Seed persistence in the soil has been proposed as a bet-hedging strategy of short-lived species to increase long-term fitness. Herbivore-induced changes in seed germination can result in a shift in the relative contribution of seeds to the seed bank and next year's generation.

The increasing expenses and environmental repercussions associated with phosphorus (P) fertiliser underscore the necessity for precision-managed application methods. These changes affect pastoral systems, where cool-season grasses like perennial ryegrass and meadow fescue form beneficial relationships with Epichlo & euml; endophytes. Understanding how fertilisers influence these endophytes, host grasses, and insect pests is crucial, as Epichlo & euml; endophytes enhance resistance to some herbivorous insects. This study examined the indirect impact of various P fertiliser regimes on cool-season grasses, which serve as food sources for porina larvae (Wiseana copularis), a significant pasture pest in New Zealand. Endophyte-infected (Epichlo & euml; sp. LpTG-3 strain AR37) perennial ryegrass and meadow fescue infected with E. uncinata (strain MaxR (AR1017)), alongside their endophyte-free counterparts were grown in P-enriched soil with varying Olsen P levels (9, 18, 28, and 78 mg/L). Freeze-dried foliage was added to semi-synthetic diets and fed to porina larvae in a no-choice assay. Measurements included diet consumption, porina survival, weight gain. Measurements in foliage included fungal alkaloid concentration, fungal biomass, and plant nutrient levels. Endophyte infection of AR37 and MaxR significantly reduced porina diet consumption, larval weight gain and survival irrespective of soil Olsen P levels to the plant. Loline alkaloid concentration in MaxR-infected herbage increased with increasing soil Olsen P levels while fungal mass remained unchanged. In endophyte-free grasses, porina larvae significantly increased their diet consumption, weight gain and survival as the Olsen P level available to the host plant increased. While endophyte strains AR37 and MaxR continue to protect their hosts under different Olsen P regimes, these results suggest that the improved performance of porina on endophyte-free plants is largely driven by P-induced changes in food quality. Here, we discuss the implications of porina damage in New Zealand pastures in the context of decreasing P availability.

To limit damage from insect herbivores, plants rely on a blend of defensive mechanisms that includes partnerships with beneficial microbes, particularly those inhabiting roots. While ample evidence exists for microbially mediated resistance responses that directly target insects through changing phytotoxin and volatile profiles, we know surprisingly little about the microbial underpinnings of plant tolerance. Tolerance defenses counteract insect damage via shifts in plant physiology that reallocate resources to fuel compensatory growth, improve photosynthetic efficiency, and reduce oxidative stress. Despite being a powerful mitigator of insect damage, tolerance remains an understudied realm of plant defenses. Here, we propose a novel conceptual framework that can be broadly applied across study systems to characterize microbial impacts on expression of tolerance defenses. We conducted a systematic review of studies quantifying the impact of rhizosphere microbial inoculants on plant tolerance to herbivory based on several measures-biomass, oxidative stress mitigation, or photosynthesis. We identified 40 studies, most of which focused on chewing herbivores (n = 31) and plant growth parameters (e.g., biomass). Next, we performed a meta-analysis investigating the impact of microbial inoculants on plant tolerance to herbivory, which was measured via differences in plant biomass, and compared across key microbe, insect, and plant traits. Thirty-five papers comprising 113 observations were included in this meta-analysis, with effect sizes (Hedges' d) ranging from -4.67 (susceptible) to 18.38 (overcompensation). Overall, microbial inoculants significantly reduce the cost of herbivory via plant growth promotion, with overcompensation and compensation comprising 25% of observations of microbial-mediated tolerance. The grand mean effect size 0.99 [0.49; 1.49] indicates that the addition of a microbial inoculant increased plant biomass by similar to 1 SD under herbivore stress, thus improving tolerance. This effect was influenced most by microbial attributes, including functional guild and total soil community diversity. Overall, results highlight the need for additional investigation of microbially mediated plant tolerance, particularly in sap-feeding insects and across a more comprehensive range of tolerance mechanisms. Such attention would round out our current understanding of anti-herbivore plant defenses, offer insight into the underlying mechanisms that promote resilience to insect stress, and inform the application of microbial biotechnology to support sustainable agricultural practices.

BACKGROUND: Fertiliser applications are well-established tools in pasture-based agricultural landscapes. This study focuses on the impact of phosphorus (P) fertiliser on grass grub (Costelytra giveni), a major pasture pest. This research investigates the interplay between P, plant growth, and grass grub fitness in Epichlo & euml; endophyte-infected perennial ryegrass (Epichlo & euml; sp. LpTG-3 strain AR37) and meadow fescue infected with E. uncinata (strain MaxR; AR1017), alongside their endophyte-free counterparts. In a glasshouse trial, plants were grown in P-enriched soil with varying Olsen P levels (9, 18, 28 or 78 mg L-1), and grass grubs were introduced. Their survival and weight gain, and plant performance were measured. In a bioassay, grass grubs were placed in specimen vials with P-enriched soils (Olsen P levels 9, 18, 28 and 78 mg L-1) and provided with identical plant material to assess their diet consumption and weight gain. RESULTS: In the glasshouse trial, results highlighted a notable decrease in the survival of grass grub on plants infected with MaxR endophyte, but not with AR37, as well as increasing soil Olsen P levels in both plant species. While grass grub decreased plant performance at the low Olsen P level (9 mg L-1), this effect diminished with increasing P. Likewise, results from the bioassay showed a decrease in diet consumption with increasing soil Olsen P levels. In both trials increasing Olsen P levels correlated with diminished grass grub performance, revealing a nuanced relationship between soil fertility and pest dynamics. CONCLUSION: The study underscores the pivotal role of selected Epichloe endophyte-grass associations in mitigating grass grub damage across varying phosphorus levels. This study highlights the potential to integrate P applications for sustainable pest control against grass grub. Further field trials are required to validate these findings. (c) 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.