Al toxicity is the main limiting factor for crop production in acidic soil, so this study is aimed to improve understanding of the effects of curcumin on the aluminum (Al) tolerance of grapes, Al-tolerant cultivation and the epigenetic mechanism of grapes exposed to Al. Vitis vinifera x V labrusca `Shuifing' cuttings were cultivated in greenhouse, which were exposed to 20 mmol L-1 aluminum sulfate and then treated with curcumin in different concentrations. Then, indicators of physiological resistance and the DNA methylation level of the grape leaves were measured. The results demonstrated that Al stress led to a series of physiological and biochemical changes in grape leaves and significantly increased DNA methylation levels. Specifically, the chlorophyll, protein, phosphorus (P) and potassium (K) content decreased, activity of superoxide dismutase (SOD) and peroxidase (POD) also decreased, while the proline, malonaldehyde and Al content increased drastically, resulting in damage to grape plants. However, treatment by 100 and 200 mu cool L-1 of curcumin led to significantly reduced DNA methylation levels and Al accumulation in grape leaves, reduced accumulation of malonaldehyde and proline, increased chlorophyll and protein content, enhanced SOD and POD activity, and improved intake of P and K. In summary, treatment by 100 and 200 mu mot Li curcumin had a significant effect on the Al tolerance of grapes, indicating that toxicity for grape cultivation in acidic soil.