Invasive weeds cause substantial ecological, economical, and social problems, and are currently being controlled by herbicide applications. However, how herbicides affect other ecological interactions of invasive weeds, including their symbiosis with arbuscular mycorrhizal fungi (AMF), remains poorly understood. In this study, we therefore conducted field investigation to understand how the herbicide glyphosate affects the AMF diversity in the rhizosphere of the invasive weed Solidago canadensis. We also performed a greenhouse experiment to study if AMF can contribute to herbicide resistance. The results showed that the AMF colonization rate was significantly higher in S. canadensis when exposed to glyphosate in the field or in greenhouse settings. AMF diversity was also found to be higher in the rhizosphere soil after glyphosate application in the field. AMF colonization in greenhouse experiments also positively correlated with plant growth and reduced amounts of damaged leaves and the plant's content of the stress markers flavonol and anthocyanin. Chlorophyll content was significantly enhanced by AMF colonization, regardless of glyphosate application. These results indicate that herbicide can promote AMF colonization and diversity, and that AMF can enhance the herbicide resistance of S. canadensis. These findings suggest that herbicide application may promote the spread of S. canadensis through enhanced microbial interactions, posing new eco-environmental risks.

Multiple pesticides often coexist in soil, potentially leading to interactions among their components, these may detrimentally impact soil organisms. This study assessed the potential risks posed by simultaneous exposure to atrazine (ATR) and phoxim (PHO) on enzyme and transcription levels in earthworms (Eisenia fetida). The results revealed that ATR exhibited higher acute toxicity towards E. fetida compared to PHO, and their combined exposure resulted in a synergistic acute effect. Furthermore, low concentration combined exposure significantly stimulated catalase (CAT), malondialdehyde (MDA), and total superoxide dismutase (T-SOD) activities, which lead to more severe oxidative damage. Elevated expression levels of translationally controlled tumor protein (tctp) and calreticulin (crt) genes were observed in most exposed groups compared to the control. The synergistic effects of ATR and PHO on earthworms observed in this study may pose ecological risks to the soil ecosystem; thus, more attention should be paid to the joint effects of different pesticides.

The soil environment has been considered capable of storing toxic substances without serious consequences for the inhabitants since plants are able to bioaccumulate pollutants without compromising their survival. The application of chemicals to increase soil productivity and the dumping of waste have worsened soil quality. Recently, following a greater awareness of the importance of monitoring the damage deriving from the consumption of contaminated crops for humans and of the protection of biodiversity, studies aimed at identifying the effects of soil contamination on terrestrial animals have increased considerably. Studies using field lizards as model organisms fit into this scenario; this research has shed light on the uptake, accumulation, and toxicity of soil pollutants on reptiles. This review summarizes data collected on lizards of the Podarcis genus, a group of resilient wild species capable of living in both pristine and anthropized areas; the data reveal that many of the effects recorded in lizard tissues at the molecular, biochemical, and histological levels are independent of the chemical composition of the contaminants and are mostly linked to the type of cellular response. Overall, these studies confirm Podarcis lizards as a good model system in ecotoxicological and cytotoxicological research, providing an accurate description of the effects of pollutants, clarifying the defense mechanisms activated in relation to different exposure routes and, finally, providing predictive information on the risks faced by other animals. Since the effects recorded in lizards have often also been observed in mammals, it can be concluded that the results obtained from studies on these animals can be translated to other terrestrial vertebrates, including mammals.

To comprehensively understand the toxic effects and ecological risks of microplastics on major economic tree species, a pot experiment was conducted using polylactic acid (mPLA) microplastics as the test object to explore the effects of different concentrations (0.1%, 0.5%, 1%, 5%, 10%, w/w, mass fraction) of microplastics on the growth and physiological characteristics of mulberry trees. The study results showed that, compared with the control group, the biomass, total chlorophyll content, and net photosynthetic rate of mulberry trees in the mPLA treatment group were significantly reduced under high concentration (10%) treatment; the activities of SOD and CAT and the MDA content were significantly increased by 50.00%, 47.83%, and 60.87%, respectively, at a 10% concentration. The results indicate that the toxic effects of microplastic addition on mulberry trees are related to the type and concentration of microplastics. High concentrations of mPLA can damage the photosynthetic system of plants, affecting photosynthesis, causing oxidative damage and thus inhibiting the growth of mulberry plants.

The escalating global issue of soil pollution by heavy metals, particularly incinerated municipal solid waste fly ash (IMSWFA), necessitates effective remediation strategies. The prevailing approach for safely disposing and utilization of IMSWFA involves high-temperature sintering. In this work, we propose a cost-effective method to produce ceramsites by utilizing IMSWFA, municipal sludge (MS), contaminated soil (CS), and iron tail slag (ITS). After conducting a comprehensive analysis and comparison of outcomes obtained from orthogonal experiments and single-factor experiments, it was determined that the optimal preparation conditions for achieving desirable results are preheating at a temperature of 400 degrees C for 15 min followed by sintering at a temperature of 1150 degrees C for 10 min. The optimal ratio of raw materials for ceramsites is 15 % IMSWFA, 15 % MS, 58 % CS, and 12 % ITS. The ceramsites, prepared in accordance with the specified process and raw material ratio, exhibit remarkable properties including robust stability, minimal water absorption, reduced weight, and elevated cylindrical compressive strength. The ceramsites demonstrate an exceptionally high heavy metal loss ratio ranging from 91 % to 100 %, while exhibiting significantly lower leaching quantities of these metals compared to the raw materials. Additionally, aging tests of ceramsites were performed under UV light and acid/alkaline etching to simulate the real-world environment. This work can be utilized to investigate the long-term environmental impact of ceramsites derived from municipal solid waste (MSW), thereby making a valuable contribution to the advancement of solid waste management technology.

Phytoremediation of oil pollution using free-floating aquatic plants is a promising method for water body cleaning. In this study, the influence of Eichhornia crassipes and Pistia stratiotes on the degradation of oil pollution was investigated. The loss of oil alkanes and the rheological characteristics of water were evaluated, and an analysis of the emerging rhizospheric microbial communities was carried out using high-throughput sequencing. The presence of E. crassipes and P. stratiotes plants in oil-contaminated tanks had no effect on the degradation of oil alkanes. However, the presence of plants promoted the development of rhizospheric bacteria capable of growing in oil-contaminated environments. Alpha diversity of microbial communities in oil-contaminated samples was higher in the presence of plants. Additionally, plants significantly reduced the water/oil interfacial tension, which facilitated the availability of hydrocarbons for biodegradation. A difference was noted in the microbiome between E. crassipes and P. stratiotes. Changes in the composition of microbial communities highlight the potential of E. crassipes and P. stratiotes as rhizospheric hosts for microorganisms in the phytoremediation of water bodies.

Purpose: Modifying soil surface conditions is essential in managing water and soil resources. Although the use of soil microorganisms in the bioengineering management in soil and water conservation has been confirmed, the controllability of the surface cracks morphometry on petroleum-contaminated soils using microorganisms has yet to be studied. Therefore, this study aimed to investigate the effect of inoculating soil microorganisms on changing the surface cracks' pattern of soil contaminated with petroleum.Materials and methods: In 2022, the studied soil was taken from Iran's Tehran Oil Refinery area from the top 30 cm of the earth's surface and transferred to the Rain and Erosion Simulation Laboratory of Tarbiat Modares University at the Faculty of Natural Resources. The predominant cyanobacteria of Microcoleus sp. Oscillatoria sp. Lyngbya sp. Nostoc sp, and Phormidium sp. bacteria of Bacillus sp. and Azetobacter sp. fungus of Penicillium oxalicum were identified, purified and proliferated. The study was conducted at a 0.5 x 0.5-m plot scale in two replications for four treatments. Uncontaminated and gasoil-contaminated soils, during one month, were inoculated by bacteria, cyanobacteria, and fungus. The soil was contaminated using 720 mL of gasoil evenly sprayed. The morphometric characteristics of soil surface cracks were investigated using the PCAS software.Results and discussion: Results indicated that soil microorganisms influence the soil cracking patterns. So, the mean area, length, and width of cracks increased by 99.55%, 50.90%, and 29.60% in uncontaminated plots inoculated with cyanobacteria compared to the control treatment. In gasoil treatment, fungus-inoculated soils performed better than control plots, and the mean area and length increased by 19.51% and 29.58%, respectively, while width decreased by 17.29%. Microorganisms caused the agglutination of aggregates by secreting enzymes and exopolysaccharides, stabilizing soil particles, generating cracks, covering the soil surface, and improving the soil's rheological properties.Conclusion: Finally, the results of the present study showed that modifying the surface of the petroleum-contaminated soil by inoculating soil microorganisms is a way to improve the hydrologic and rheologic characteristics of the soil.

Petroleum pollution in soil is very damaging to the areas affected by the accidental release of petroleum hydrocarbons and has destructive impacts on natural resources and environmental health. Therefore, its monitoring and analysis are critical, however, due to the cost and time associated with chemical approaches, finding a quick and cost-effective analytical method is valuable. This study was conducted to evaluate the potential of using visible near infrared (Vis-NIR) spectroscopy to predict total petroleum hydrocarbons (TPH) in polluted soils around the Shadegan ponds, in southern Iran. One hundred soil samples showing various degrees of pollution were randomly collected from topsoil (0-10 cm). The soil samples were analyzed for TPH using Vis-NIR reflectance spectroscopy in the laboratory and then following application of preprocessing transformation, partial least squares PLS regression as well as two machine learning models including random forest (RF), and support vector machine (SVM) were examined. The results showed that the reflectance values at 1725 nm and 2311 nm, respectively, served as indicative TPH reflectance features, exhibiting weaker reflection with rising TPH. Among the preprocessing methods, the baseline correction method indicated the highest performance than the others. According to the evaluation model criteria in the validation dataset, the efficiency of the three selected models was observed in the following order SVM > RF > PLS regression. The SVM model provided the best performance in the validation dataset with r(2) = 0.85, root mean of square (RMSEP = 1.59 %, and the ratio of prediction to deviation (RPD) = 2.6. Overall, this study provided strong evidence supporting the considerable potential of Visible-NIR spectroscopy as a rapid and cost-effective technique for estimating TPH levels in oil-contaminated soils, surpassing traditional chemical analytical methods. Applying the mid-infrared spectrum (MIR) in combination with Visible-NIR data is expected to provide more comprehensive and accurate results when assessing soils in polluted areas, thereby enhancing the accuracy and reliability of the results across a diverse range of soil types.

In recent decades, human actions aimed at maintaining dominance over the earth's natural resources have endangered the ecological balance. The irreparable damage caused by oil pollution not only impacts the environment but also affects the economy and the health of living organisms. Therefore, in addition to preventive measures, it is crucial to focus on techniques for removing this type of pollution. Bioremediation, which involves the use of microorganisms like bacteria and fungi to eliminate, decompose, or reduce pollution, offers eco-friendly and cost-effective advantages. While organisms from various domains can play a role in bioremediation, fungi stand out as promising candidates. Their metabolic abilities enable them to breakdown complex molecules, making them highly effective in degrading stubborn organic matter in nature. Fungi, often referred to as mycoremediators, have been more extensively studied for bioremediation in terrestrial ecosystems compared to aquatic environments. This review explores the research conducted on fungi and bioremediation, summarizing findings and analyzing the progress in this field from the past to the present through bibliometric analysis. The results indicate that most studies concentrate on fungi isolated from soil, with Aspergillus, Penicillium, Fusarium strains being frequently reported. China, Iran, and Nigeria have published the most documents on this subject in the Scopus database. Utilizing a consortium of microorganisms is likely to yield better results than using a single strain. Overall, the use of fungi in bioremediation shows promise due to their specific metabolic characteristics.

The study explores the aftermath of a wastewater reservoir failure in a phosphate fertilizer industry, resulting in the release of acidic water containing phosphorus and sulfate compounds into the Ashalim stream's Nature Reserve in the Judean desert, which affected the soil surface biological crusts (biocrusts) layer. The study aims to examine contamination effects on biocrusts over 3 years at two research sites along the stream, compare effects between contaminated sites, assess rehabilitation treatments, and examine their impact on soil characteristics. Hypotheses suggest significant damage to biocrusts due to acidic water flow, requiring human intervention for accelerated restoration. The results indicate adverse effects on biocrust properties, risking its key role in the desert ecosystem. The biocrust layer covering the stream's ground surface suffered significant physical, chemical, and biological damage due to exposure to industrial process effluents. However, soil enrichment treatments, including biocrust components and organic material, show promising effects on biocrust recovery.