Background: The chickpea, scientifically known as Cicerarietinum L., is a significant legume crop that serves as a valuable source of vegetable protein. The chickpea crop is susceptible to various pests and illnesses. Collar rot, induced by the fungal pathogen Sclerotium rolfsii, is a highly significant and extremely damaging disease that affects chickpea crops. The disease causes seedling mortality ranging from 54.7 to 95 per cent and field conditions result in yield decrease ranging from 22 to 50 per cent. The present study aimed to investigate the effectiveness of using a combination of fungicides, bio-agents and organic amendments for the management of collar rot in chickpea. Methods: The investigations were conducted in the Rabi seasons of 2019-20 and 2020-21. The experiments involved the integration of fungicides, fungal biocontrol agents (Trichoderma spp.), FYM and vermicompost to control Collar rot disease in Chickpea caused by S. rolfsii. Six indigenous fungal antagonists (Trichoderma spp.) were assessed in a laboratory setting against S. rolfsii using both dual culture and non-volatile (culture filtrate) methods. The efficacy of the fungicides was assessed using the poison food technique. Nine fungicides were assessed in a laboratory setting to determine their effectiveness against a pathogen and Trichoderma harzianum-2. The fungicides were tested at four different concentrations: 50, 100, 500 and 1000 ppm. The goal was to identify fungicides that are extremely toxic to S. rolfsii at lower concentrations, while being less harmful to the bioagent Trichoderma spp. Pot culture studies were conducted using a completely randomised design (CRD), while field experiments were conducted using a randomised block design (RBD). Result: Trichoderma harzianum-2 (TH-2) was found to be highly efficient against the pathogen. It reduced the growth of the pathogen by 75.18% in the dual culture technique and by 61.85% in the culture filtrate approach. Among the nine fungicides tested, four of them, specifically propineb, mancozeb, captan 70% + hexaconazole 5% WP and penflufen 13.28% w/w + trifloxystrobin, showed lower inhibitory effects on Trichoderma harzianum at doses ranging from 50to 1000 ppm. The treatment that resulted in the highest seed germination rate (100%) and the lowest occurrence of collar rot was the one where the seeds were treated with captan 70% + hexaconazole 5% WP and the soil was supplemented with Trichoderma harzianum through vermicompost application.

This study investigated the sub-lethal effects of four commercial fungicides-two foliar (Amistar (R) Xtra and Mirador (R)) and two ear fungicides (Prosaro (R) and Icarus (R))-applied alone and in combination to wheat crops on caged earthworms (Eisenia fetida). We measured biomarkers that included detoxification responses (glutathione S-transferase, GST), oxidative stress levels (lipid peroxidation, LPO, and catalase, CAT), DNA damage (comet assay), energy reserves (lactate dehydrogenase, LDH), and immune response (lysozyme activity, LYS). The absence of significant differences in catalase and lipid peroxidation levels suggested no oxidative stress due to fungicide exposure. However, the foliar fungicide Amistar (R) Xtra induced the highest GST activity and DNA fragmentation, suggesting synergistic effects between its active ingredients and undisclosed co-formulants. Similar effects observed with the Amistar (R) Xtra-Prosaro (R) mixture confirmed the greater toxicity of Amistar (R) Xtra. This study provides novel insights into the sub-lethal effects of single and combined commercial fungicides on a standard toxicity test organism, shedding light on the ecological implications of fungicide use in agroecosystems and reinforcing the need for pesticide reduction.

Naphthalene is a fungicide that can also be a phase-change agent owing to its high crystallization enthalpy at about 80 degrees C. The relatively rapid evaporation of naphthalene as a fungicide and its shape instability after melting are problems solved in this work by its placement into a cured epoxy matrix. The work's research materials included diglycidyl ether of bisphenol A as an epoxy resin, 4,4 '-diaminodiphenyl sulfone as its hardener, and naphthalene as a phase-change agent or a fungicide. Their miscibility was investigated by laser interferometry, the rheological properties of their blends before and during the curing by rotational rheometry, the thermophysical features of the curing process and the resulting phase-change materials by differential scanning calorimetry, and the blends' morphologies by transmission optical and scanning electron microscopies. Naphthalene and epoxy resin were miscible when heated above 80 degrees C. This fact allowed obtaining highly concentrated mixtures containing up to 60% naphthalene by high-temperature homogeneous curing with 4,4 '-diaminodiphenyl sulfone. The initial solubility of naphthalene was only 19% in uncured epoxy resin but increased strongly upon heating, reducing the viscosity of the reaction mixture, delaying its gelation, and slowing cross-linking. At 20-40% mass fraction of naphthalene, it almost entirely retained its dissolved state after cross-linking as a metastable solution, causing plasticization of the cured epoxy polymer and lowering its glass transition temperature. At 60% naphthalene, about half dissolved within the cured polymer, while the other half formed coarse particles capable of crystallization and thermal energy storage. In summary, the resulting phase-change material stored 42.6 J/g of thermal energy within 62-90 degrees C and had a glass transition temperature of 46.4 degrees C at a maximum naphthalene mass fraction of 60% within the epoxy matrix.

Seed coating with fungicides is a common practice in controlling seed-borne diseases, but conventional methods often result in high toxicity to plants and soil. In this study, a nanoparticle formulation was successfully developed using the metal-organic framework UiO-66 as a carrier of the fungicide ipconazole (IPC), with a tannic acid (TA)-ZnII coating serving as a protective layer. The IPC@UiO-66-TA-ZnII nanoparticles provided a controlled release, triggered and regulated by environmental factors such as pH and temperature. This formulation efficiently controlled the proliferation of Fusarium fujikuroi spores, with high penetration into both rice roots and fungal mycelia. The product exhibited high antifungal activity, achieving control efficacy rates of 84.09% to 93.10%, low biotoxicity, and promoted rice growth. Compared to the IPC flowable suspension formula, IPC@UiO-66-TA-ZnII improved the physicochemical properties and enzymatic activities in soil. Importantly, it showed potential for mitigating damage to beneficial soil bacteria. This study provides a promising approach for managing plant diseases using nanoscale fungicides in seed treatment. IPC-loaded UiO-66 with tannic acid-ZnII shells for precision management of rice seedling disease through intelligent, responsive release.A pH- and temperature-sensitive, controlled-release nanoparticle system was developed.Tannic acid-ZnII-modified nanoparticles penetrate into rice roots and fungal mycelium.Nanoparticles provide better control of Fusarium fujikuroi and promote seedling growth.Nanoparticles reduce the pollution of soil environment by conventional seed coatings.

Allium species are known for their culinary, medicinal, and ornamental purposes. Fusarium basal rot is one of the most damaging soilborne fungal diseases of Allium species and poses a significant threat to yield, quality, and storage life worldwide. Various species of Fusarium have been identified as causal agents for Fusarium basal rot, depending on the Allium species involved. Diverse disease management practices have been implemented to mitigate the impact of Fusarium basal rot. This review article provides a comprehensive overview of the recent progress in detecting different species of Fusarium involved in Fusarium basal rot and strategies to control them in affected Allium species involving chemical, biological, and cultural methods. It covers the latest advancements in host plant resistance research from traditional breeding to modern molecular techniques and studying secondary metabolites involved in defense mechanisms against Fusarium basal rot.

The increasing popularity of seed treatment applications in agriculture may leave unintended hazards to soil biota, such as earthworms. The objective of this study was to explore mitochondrial DNA toxicity resulting from sublethal exposures to systemic pesticides, including four neonicotinoid insecticides (neonics), as well as coexposure to difenoconazole (DIF), a triazole fungicide, in earthworms (Eisenia fetida) in vivo. Earthworms were exposed under dose regimes resembling label recommendation and levels left in soil post seed treatment application for 30 days in an earthworm breeding facility. Mitochondrial DNA copy numbers (mtDNAcn) in earthworms were determined by using the 2(-Delta Ct) algorithm. Earthworms' body weights were recorded before and after the exposure period. We found a highly significant increase of mtDNAcn in earthworms across all exposure groups (ANOVA, p < 0.001), either under a single neonic or combined with DIF exposure. Coupled with mtDNA toxicity, earthworms in the treatment groups gained significantly less weight than control earthworms (ANOVA, p < 0.001). We concluded that systemic pesticides, both neonics and DIF, posed mtDNA toxicity as measured by mtDNAcn, in earthworms.