This study introduces a novel method for stabilising expansive subgrade soils by integrating microbially induced calcite precipitation (MICP) process with a synergistic combination of waste sugarcane bagasse and recycled polyester fibres. This innovative approach aims to enhance strength properties and reduce volume susceptibility. The study demonstrates increases in Unconfined Compressive Strength (UCS), Split Tensile Strength (STS), and California Bearing Ratio (CBR), while substantially decreasing linear shrinkage, swell strains and pressures, indicating improved soil stability. The study also investigates the microstructural and chemical transformations through SEM-EDS, FTIR, and DSC-TGA, further corroborated by 16S metagenomic sequencing to understand microbial dynamics. Optimal stabilisation results were obtained with 0.5% fibre content and a four-day mellowing period, enhancing soil structure and durability by calcite precipitation and leveraging the combined benefits of natural and synthetic fibres. These fibres strengthen the soil structure and facilitate calcite nucleation, ensuring lasting stability, particularly valuable for stabilising expansive subgrade soils.

Expanding on the challenges of expansive soils to civil infrastructure, this research delves into the synergistic application of microbially induced calcium carbonate precipitation (MICP) through bio-stimulation and natural fiber reinforcement to mitigate soil swell-shrink behavior and enhance soil strength. This research diverges from traditional methods by addressing their economic and environmental limitations. The dual strategy of bio-stimulation with natural fiber reinforcement was assessed through laboratory tests, including unconfined compression, 1D swell, linear shrinkage tests, and microstructural analysis. This methodology involved preparing solutions to foster bacterial growth and strategically adding jute fibers to enhance the soil matrix. Results revealed significant improvements in soil strength (up to 186%), and reductions in swell strain (up to 85%) and swell pressure (up to 90%), with the optimal jute fiber content at 1.5%. Additionally, a significant increase in calcium carbonate content (163-176%) highlighted bio-stimulation's role in soil stabilization. SEM analysis showed that bio-stimulation and jute fiber reinforcement transformed the soil microstructure, enhancing cohesion and reducing deformability. These outcomes highlight the promise of combining bio-stimulated MICP with natural fiber reinforcement as an eco-friendly and efficient approach to soil stabilization. They also add to the growing body of knowledge on tackling the issues posed by expansive soils in civil engineering applications.

Industrial waste and sewage deposit heavy metals into the soil, where they can remain for long periods. Although there are several methods to manage heavy metals in agricultural soil, microorganisms present a promising and effective solution for their detoxification. We isolated a rhizofungus, Aspergillus terreus (GenBank Acc. No. KT310979.1), from Parthenium hysterophorus L., and investigated its growth-promoting and metal detoxification capabilities. The isolated fungus was evaluated for its ability to mitigate lead (25 and 75 ppm) and copper (100 and 200 ppm) toxicity in Triticum aestivum L. seedlings. The experiment utilized a completely randomized design with three replicates for each treatment. A. terreus successfully colonized the roots of wheat seedlings, even in the presence of heavy metals, and significantly enhanced plant growth. The isolate effectively alleviates lead and copper stress in wheat seedlings, as evidenced by increases in shoot length (142%), root length (98%), fresh weight (24%), dry weight (73%), protein content (31%), and sugar content (40%). It was observed that wheat seedlings possess a basic defense system against stress, but it was insufficient to support normal growth. Fungal inoculation strengthened the host's defense system and reduced its exposure to toxic heavy metals. In treated seedlings, exposure to heavy metals significantly upregulated MT1 gene expression, which aided in metal detoxification, enhanced antioxidant defenses, and maintained metal homeostasis. A reduction in metal exposure was observed in several areas, including normalizing the activities of antioxidant enzymes that had been elevated by up to 67% following exposure to Pb (75 mg/kg) and Cu (200 mg/kg). Heavy metal exposure elevated antioxidant levels but also increased ROS levels by 86%. However, with Aspergillus terreus colonization, ROS levels stayed within normal ranges. This decrease in ROS was associated with reduced malondialdehyde (MDA) levels, enhanced membrane stability, and restored root architecture. In conclusion, rhizofungal colonization improved metal tolerance in seedlings by decreasing metal uptake and increasing the levels of metal-binding metallothionein proteins.