In this study, we present an on-chip analytical method using a microfluidic device to characterize the mechanical properties in growing roots. Roots are essential organs for plants and grow under heterogeneous conditions in soil. Especially, the mechanical impedance in soil significantly affects root growth. Understanding the mechanical properties of roots and the physical interactions between roots and soil is important in plant science and agriculture. However, an effective method for directly evaluating the mechanical properties of growing roots has not been established. To overcome this technical issue, we developed a polydimethylsiloxane (PDMS) microfluidic device integrated with a cantilevered sensing pillar for measuring the protrusive force generated by the growing roots. Using the developed device, we analyzed the mechanical properties of the roots in a model plant, Arabidopsis thaliana. The root growth behavior and the mechanical interaction with the sensing pillar were recorded using a time-lapse microscopy system. We successfully quantified the mechanical properties of growing roots including the protrusive force and apparent Young's modulus based on a simple physical model considering the root morphology. (c) 2025 Institute of Electrical Engineers of Japan and Wiley Periodicals LLC.
Shale formations have recently gained plenty of attention owing to their large amounts of reserves. Horizontal drilling and hydraulic fracturing are the proposed approaches for the development of shale formations. The extended information of the mechanical properties of shale formation is crucial for designing a successful hydraulic fracturing operation. On the other hand, the mechanical properties of such organic-rich formations are greatly affected by the mechanical characteristics of the present kerogen (organic matter), which dramatically changes during the maturation process. In this study, a Qingshankou shale sample containing kerogen type I is mechanically investigated at different maturity levels using the grid nanoindentation approach. To this end, the original immature sample is artificially matured during hydrous (HP) and anhydrous (AHP) pyrolysis. More than 930 nanoindentation tests were performed on grids of 9 x 8 on the surface of 13 samples with different maturities. The test results showed that the presence of water during pyrolysis can significantly affect the shale sample's mechanical characteristics. In higher temperatures and higher levels of maturity, the role of water becomes more pronounced. During hydrous pyrolysis, kerogen produces larger amounts of oil and bitumen, which become progressively porous. While the original sample showed a Young's modulus value of more than 48 GPa, and it fluctuated between approximately 19 and 32 GPa during the HP scenario and between 17 and 34 GPa during the AHP process. In terms of hardness, the original sample exhibited an initial value of about 1.1 GPa and more mature samples reflected hardness values in the range of approximately 0.3 and 0.97 GPa in both scenarios. According to the trends of mechanical properties during maturation, mechanical properties decreased at the initial stage of maturation and remained relatively constant during the oil window. Then, another decline was detected at the wet-gas window's closure. In the dry-gas window, HP and AHP scenarios exhibited different behaviors mainly due to the chemical structure of the kerogen residue. 2025 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Published by Elsevier B.V. This an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).
Inherent (fabric) anisotropy is one of the most important properties of earthen materials that significantly influences their strength and stiffness characteristics. In this study, a comprehensive series of unconfined and constrained compression tests is performed on normally consolidated (NC) clay samples with different plasticity indices to examine the effect of inherent anisotropy on their mechanical characteristics. Accordingly, several cylindrical clay samples with different proportions of kaolinite and bentonite are reconstituted at a wide range of deposition angles, and then subjected to both unconfined and constrained compressive loadings. The experimental results reveal that, for a clay sample with a particular plasticity index, the highest and lowest values of unconfined compressive strength (UCS), secant modulus (E50), and constrained Young's modulus (Eoed) are associated with deposition angles of 0 degrees and 90 degrees, respectively. The results also show that at a certain bedding plane angle, the sample containing 30 % bentonite (PI = 110 %) exhibits the highest UCS, E50, and Eoed values. Several practical empirical correlations are developed to estimate the strength and stiffness properties of NC clays based on their plasticity indices and bedding plane directions. Furthermore, Scanning Electron Microscopy (SEM) analysis is conducted to explore the microstructure of samples containing varying percentages of kaolinite and bentonite.
The present research aims to examine the influence of stress anisotropy on the small-strain Young's modulus of granular materials. To this end, a series of numerical tests, including isotropic compression, isotropic extension, triaxial extension (TE), triaxial compression (TC), reduced triaxial extension, and reduced triaxial compression tests, were conducted using the 3D Discrete Element Method. The findings revealed the presence of a threshold value of stress ratio (SR) in both TE and TC stress paths. When the SR falls within the range between two threshold values, the fabric of the specimen, represented by the contact normal distribution, remains nearly constant. In this range, the anisotropy of small-strain Young's modulus is primarily attributed to the anisotropy of stress. However, it was observed that the small-strain Young's modulus in a specific direction is affected by the value of SR, contradicting the prevailing belief that it is solely influenced by the principal stress in that direction. A novel factor R-s was introduced and incorporated into Hardin's equation to capture the influence of the SR on the small-strain Young's modulus. In addition, a relationship was established between mechanical coordination number and small-strain Young's modulus during triaxial tests by considering both stress anisotropy and fabric anisotropy.
Starch blended low-density polyethylene (LDPE) has been extensively used to produce packaging film, but it has very low mechanical properties. This work emphasises the extraction of nanosilica from rice husk as a property-enhancing filler for producing high-quality packaging material. Nanosilica (200 nm) was obtained by chemical treatment followed by further size reduction through cryomill. The obtained nanomaterial was found to have a high surface area (189.64 m(2)/g) and pore volume (.462 cc/g) with high compatibility with the other materials in the matrix. The SEM and TEM analysis indicates the uniformity in particle size of the nanomaterial with an agglomerating tendency. The X-ray diffractometer (XRD) and fourier transform infrared spectroscopy (FTIR) analysis reveals that the obtained material is amorphous in nature. The nanomaterial is dispersed in various proportions in LDPE/starch matrix, and it is observed that the highest tensile strength (9.62 MPa) can be obtained at 1.5% nanosilica content in the matrix. A continuous increase in Young's modulus and stiffness from 372.3 to 440.12 MPa and 20 243.2 to 28 559.42 N/m, respectively, when 1.5% of nanosilica is dispersed in the biodegradable matrix. Garden soil was a better degrading medium for the sample containing 20% of starch with weight loss of 10.32% and reduction of tensile strength and tear strength values to 5.987 MPa and 99.165 N/mm respectively, in 1 year.
The shallow seismic methods, including seismic refraction and 1D MASW, were used to investigate the shallow soil in the vicinity of five damaged building blocks in the village of El-Kalaheen. These building blocks exhibited structural problems including cracks, fissures and displacements between neighboring buildings. The results of both methods show that the shallow subsurface consists of two layers: a surface layer of loose sands, gravels, silts and clays and a compacted sandy clay layer that forms the bedrock in the area. The resulting seismic velocities were used to calculate the geotechnical parameters of the two layers, including Poisson's ratio, shear modulus, Young's modulus, material index and N-value. In addition, the shear wave velocities resulting from the 1D MASW method were used to calculate the average Vs30 in the site. The calculated values of the geotechnical parameters show a gradual increase in the competence of the upper layer from fairly competent and loose in the south of the area to competent and denser in the north. The geotechnical parameters of the bedrock also show an increase from moderately competent in the south to denser and more competent in the north. Possible zones of weakness are also observed in the southern part of the site. The calculated Vs30 indicates a site with stiff soil classification.