This study investigates the potential of Termite Hill Soil (THS) as a sustainable partial replacement for Ordinary Portland Cement (OPC) in mortar production. The experimental program involved preparing mortar mixtures with 0%, 5%, 10%, 15%, and 20% THS replacement levels by weight while maintaining a constant water-to-cement ratio. Various physical, chemical, fresh, mechanical, durability and microstructural properties were studied. The findings indicate that THS possesses pozzolanic properties. Workability and setting time decreased, while the water demand for normal consistency increased as THS content rose. The compressive strength results showed a 9.5% improvement at 5% THS and achieved 98% of the control mix at 10% THS. Additionally, THS5 and THS10 significantly enhanced bulk density, ultrasonic pulse velocity, and compressive strength, particularly in the latter ages of mortar. The microstructural analysis confirmed improved density in these mixtures, along with respectable thermal properties and FTIR spectra. Overall, THS showcases promising and commendable performance, and its utilization as a substitute for OPC not only reduces the carbon footprint of cement production but also promotes sustainability within the construction industry.