Flow instability impacts negatively on hydraulic structures. Changes in water pressure or the periodic impact of water flows cause structural damage to channels. The rapid increase in water depth leads to overflows or sprays, which erode soil adjacent to channels. In this study, flow instability was examined through the basis of theories and experiments. The theoretical discriminants for flow instability were inferred by Vedernikov number and the effect of slopes on the Froude number was considered. A rectangular cross- channel was selected for the experiments. The experimental results were compared with theories, it was shown that when the flow conditions were on the margin of instability, the discriminant established by this study is able to accurately determine the occurrence of instability. Through this new discriminant, the discrepancy which appears in traditional method can be avoided. The presented results are ideal for channel design and offer new approaches for flow instability prevention.