Pesticide application in agriculture has significantly increased to enhance crop productivity. However, its longterm use raises concerns regarding soil microbial communities and water resource contamination. This study examines the prolonged impacts of agropharmaceutic (pesticide category) residues on microbial diversity, soil health, and groundwater pollution. The findings reveal that persistent agropharmaceutic exposure alters microbial community structure, reducing beneficial microorganisms while promoting resistant strains. Additionally, agropharmaceutic leaching into water systems contributes to ecological disturbances and human health risks. This research underscores the urgent need for sustainable pest management practices to mitigate environmental damage while maintaining agricultural efficiency.

Global climate change is altering the amounts of ice and snow in winter, and this could be a major driver of soil microbial processes. However, it is not known how bacterial and fungal communities will respond to changes in the snow cover. We conducted a snow manipulation experiment to study the effects of snow removal on the diversity and composition of soil bacterial and fungal communities. A snow manipulation experiment was carried out on the meadow steppe in Hulunbuir, Inner Mongolia, China, during the winter period October 2019-March 2020. Soil samples were collected from the topsoil (0-10 cm) in mid-March 2020 (spring snowmelt period). Snow removal significantly reduced soil moisture and soil ammonium concentration. Lower snow cover also significantly changed the fungal community structure and beta diversity. Snow removal did not affect the bacterial community, indicating that fungal communities are more sensitive to snow exclusion than bacterial communities. The relative importance analysis (using the Lindeman-Merenda-Gold method) showed that available nitrogen (AN), soil water content (SWC), total organic carbon (TOC), microbial biomass carbon (MBC), and microbial biomass nitrogen (MBN) together explained 94.59% of the variation in soil fungal beta diversity, where AN was identified as the most important predictor. These finding provide insights into potential impacts of climate warming and associated reduced snow cover on soil microbial communities and processes.