Highlights What are the main findings? The bast fibers extracted from the second generation of energy crop L. biomass have consistent yield and stable productivity across different seasons; Sida hermaphroditaThe results revealed a favorable moisture content, strength, and toughness, suitable for storage and processing. What are the implications of the main findings? fibers are suitable for use in a wide range of industrial applications, where a combination of lightness, strength, and toughness is required; Sida hermaphroditaAccording to the circular economy principles, a high percentage of side streams after fiber isolation are successfully applied for biofuel production.Highlights What are the main findings? The bast fibers extracted from the second generation of energy crop L. biomass have consistent yield and stable productivity across different seasons; Sida hermaphroditaThe results revealed a favorable moisture content, strength, and toughness, suitable for storage and processing. What are the implications of the main findings? fibers are suitable for use in a wide range of industrial applications, where a combination of lightness, strength, and toughness is required; Sida hermaphroditaAccording to the circular economy principles, a high percentage of side streams after fiber isolation are successfully applied for biofuel production.Abstract Virginia mallow or Sida hermaphrodita (L.) Rusby (SH) is a perennial plant from the Malvaceae family (mallows) that is used for medicinal purposes, reducing soil erosion, cleaning soil, and most recently for energy production. The potential of sustainable lignocellulosic agro-waste is immense as it represents Earth's most abundant organic compound. This paper explores fibers isolated from SH stems, a plant with significant industrial application potential, including technical textiles and biocomposites. The fibers were harvested in January, March, and November of 2020 and in January and March of 2021, and their yield, mechanical properties, moisture content, and density were thoroughly analyzed. The fiber yield showed slight variations depending on the harvest time, with consistent results observed across different years, suggesting stable productivity. The SH fibers demonstrated a favorable moisture content, making them suitable for storage and processing, and their density ranged between 1.52 and 1.58 g/cm3, comparable to that of other natural fibers. According to this research, the best mechanical properties were observed in the winter harvest. Furthermore, the high percentage of solid residue left after fiber extraction shows promise for sustainable utilization, primarily for biofuel production. This study underscores the versatility and sustainability of SH fibers, positioning them as a valuable resource for a wide range of industrial applications.