Biochar has been considered a promising material for soil carbon sequestration. However, there are huge knowledge gaps regarding the carbon reduction effects of biochar-plant-polluted soil. Here, rice straw biochar (RB) was applied in ryegrass-cadmium (Cd)-contaminated soil to investigate the full-cycle carbon dioxide (CO2) emission and intrinsic mechanism. RB resulted in a 37.00 %-115.64 % reduction in accumulative CO2 emissions and a 31.61 %-45.80 % reduction in soil bioavailable Cd throughout the whole phytoremediation period. CO2 emission reduction triggered by RB can be attributed to the regulation of plant and rhizosphere ecological functions. RB could bolster photosynthetic carbon fixation by maintaining the stability of the structure of the chloroplasts and thylakoids, accelerating the consumption of terminal photosynthate, upregulating photosynthetic pigments, and mitigating oxidative damage. Besides, RB reduced the metabolism of readily mineralizable carbon sources while reinforcing the utilization of certain nutrient substrates. Besides, the composition of rhizosphere microbial communities was altered, especially those associated with carbon cycling (Chloroflexi, Actinobacteriota, and Acidobacteriota phyla) to orient soil microbial evolution to lower soil CO2 emission. This study aims to establish a win-win paradigm of carbon reduction-pollution alleviation to deepen the understanding of biochar in carbon neutrality and soil health and provide a theoretical basis for field pilot-scale studies.

Context. The incorporation of trees into integrated crop-livestock systems (ICLS) has been encouraged because of their role in climate change mitigation through plant and soil carbon sequestration. One challenge is to minimize competition (especially for light) and the damage caused by cattle to trees. Aim. This study sought to evaluate the performance of beef heifers grazing on cool-season grasses in two ICLS, crop-livestock (CL) and crop-livestock with immature Eucalyptus grandis trees (CLT), at two nitrogen (N) rates (50 and 150 kg/ha) on pasture. Because these were the first stocking seasons after tree planting, the physical impact of animals (e.g. debarking) on the trees was also evaluated. Methods. The experimental design was randomized blocks with treatments arranged in a 2 x 2 factorial scheme (2 systems x 2 N fertilization rates), with three replicates. Forage production (as dry matter, DM) and animal performance were evaluated for 2 years. Key results. Total forage production and liveweight (LW) gain per area over 117 days of grazing were on average higher for CL (6736 +/- 565 kg DM/ha and 505 +/- 58.6 kg LW/ha respectively) than for CLT (5455 +/- 372 kg DM/ha and 364 +/- 42.3 kg LW/ha), regardless of N rate, and even at similar sward heights (similar to 24 cm). The damage caused by heifers to the bark of the trees was classified as high intensity in 91.1% of the trees, even after the trees had reached a diameter at breast height of 9.9 cm. Conclusions. The interaction between livestock and trees was detrimental to the system's productivity, affecting pasture growth, animal performance and the quality of trees as sawn wood. This finding underscores the importance of selecting appropriate tree species, plant density and species arrangement in ICLS. Implications. Lower tree densities (<237 trees/ha) and preventive measures regarding the use of E. grandis in CLT systems with cool-season grasses are necessary in subtropical regions.

The understanding of rainfall-induced landslides on gentle, loose-fill slopes is limited in comparison to steep slopes. Hence, two physical model tests were conducted on silty sand slopes under continuous rainfall: one on a bare slope and the other on a slope planted with ryegrass. The slope angle of 25 degrees is much lower than the internal friction angle of slope material (34.3 degrees), which makes the model test fall well into the category of gentle slope. For the initially unsaturated bare slope, a rainfall event with return period of 18 years could trigger a rapid and retrogressive global sliding, which differs from previous findings that gentle slopes would only experience shallow failure. A sudden increase in pore-water pressure was simultaneously observed, which might be generated by the wetting-induced collapse of unsaturated loose soil. On the other hand, the stability of the slope with grass plantation was significantly enhanced, and it was able to withstand rainfall event more severe than those with a return period of 100 years, with only minimal deformation. The results suggest that the gain in shear strength due to ryegrass roots surpasses the additional sliding force caused by the increased water retention capability. Additionally, it is found that the abrupt change in pore pressure was no longer indicative of slope failure in the case of the grass-reinforced slope.

The challenge of soil salinization and alkalization, with its significant impact on crop productivity, has raised growing concerns with global population growth and enhanced environmental degradation. Although arbuscular mycorrhizal fungi (AMF) and calcium ions (Ca2+) are known to enhance plant resistance to stress, their combined effects on perennial ryegrass' tolerance to salt and alkali stress and the underlying mechanisms remain poorly understood. This study aimed to elucidate the roles of Arbuscular mycorrhizal (AM) fungus Rhizophagus irregularis and exogenous Ca2+ application in molecular and physiological responses to salt-alkali stress. AM symbiosis and exogenous Ca2+ application enhanced antioxidant enzyme activity and non-enzymatic components, promoting reactive oxygen species (ROS) scavenging and reducing lipid peroxidation while alleviating oxidative damage induced by salt-alkali stress. Furthermore, they enhanced osmotic balance by increasing soluble sugar content (Proportion of contribution of the osmotic adjustment were 34 similar to 38 % in shoots and 30 similar to 37 % in roots) under salt stress and organic acid content (Proportion of contribution of the osmotic adjustment were 32 similar to 36 % in shoots and 37 similar to 42 % in roots) under alkali stress. Changes in organic solute and inorganic cation-anion contents contributed to ion balance, while hormonal regulation played a role in these protective mechanisms. Moreover, the protective mechanisms involved activation of Ca2+-mediated-mediated signaling pathways, regulation of salt-alkali stress-related genes (including LpNHX1 and LpSOS1), increased ATPase activity, elevated ATP levels, enhanced Na+ extrusion, improved K+ absorption capacity, and a reduced Na+/K+ ratio, all contributing to the protection of photosynthetic pigments and the enhancement of photosynthetic efficiency. Ultimately, the combined application of exogenous Ca2+ and AMF synergistically alleviated the inhibitory effects of salt-alkali stress on perennial ryegrass growth. This finding suggested that exogenous Ca2+ may participate in the colonization of perennial ryegrass plants by R. irregularis, while AM symbiosis may activate Ca2+ pathways. Consequently, the combined treatment of AM and Ca2+ is beneficial for enhancing plant regulatory mechanisms and increasing crop yield under salt-alkali stress.