Although silicon nutrition in crops has been reported to improve growth and herbicide tolerance, the response of crop-associated weeds has not been studied. To support or reject the hypothesis that silicon nutrition can affect the tolerance of velvetleaf to pyrithiobac-sodium, affecting crop-weed competition, this study was conducted as a dose-response study in which cotton and velvetleaf grown in soil with or without K2SiO3 + silicate-solubilizing bacteria (SSB) were sprayed with pyrithiobac-sodium. Some enzymes involved in lignin biosynthesis, antioxidant, and herbicide metabolism were measured to find physiological changes. The findings accept the hypothesis above for the first time. Silicon nutrition could disrupt pyrithiobac-sodium selectivity for controlling velvetleaf in cotton. Regardless of treatments, velvetleaf accumulated more silicon and lignin than cotton. Unlike phenylalanine ammonia-lyase, the activity of cytochrome P450 reductase (1.3 vs. 0.7 U/g), glutathione S-transferase (1.7 vs. 1.2 U/g), superoxide dismutase (21.7 vs. 12.5 U/mg), and catalase (443.9 vs. 342.5 U/mg) was higher in cotton than in velvetleaf, grown in soil without silicon nutrition. All enzymes became more active with silicon nutrition, but the increase was higher in velvetleaf. In field studies, velvetleaf benefited from silicon nutrition more than cotton, enhancing the competitive ability of velvetleaf in cotton and reducing further crop yield. K2SiO3 + SSB caused a 29.7 % increase in velvetleaf biomass, which caused the greatest damage to cotton seed (80.9 %) and lint (69.2 %) yields. It is recommended to avoid soil nutrition with K2SiO3 + SSB in velvetleafinfested cotton fields, where velvetleaf control with pyrithiobac-sodium is intended.
Dollar spot, caused by Clarireedia jacksonii, is a chronic fungal disease of creeping bentgrass in cool, humid environments in the United States. In closely mown golf playing surfaces, symptoms include small, circular, sunken spots of blighted turf that eventually coalesce if left untreated. This report evaluates the efficacy of preventative fungicide programs to suppress dollar spot in golf greens. Programs contained broad spectrum fungicides mixed with Appear II, a systemic potassium phosphite fungicide that is formulated with a green pigment. A study was conducted on an 'L-93' plus 'Providence' creeping bentgrass (Agrostis stolonifera) push-up constructed nursery green originally seeded in 2000 at the North Shore Country Club in Glenview, IL. Results indicated fungicide programs that contained Appear II can provide complete control of dollar spot and can also significantly reduce localized dry spot, an abiotic disorder of turfgrass caused by hydrophobic soils, which commonly occurs in sand-based putting greens.
Drought significantly reduces cotton boll yields across various fruiting branches (FBs). Potassium (K) application can partially mitigate the drought-induced damage by modifying the biosynthesis of photoassimilates in the leaf subtending to cotton boll (LSCB) and facilitating their transport to the subtending bolls, although its effects vary among FBs. The underlying mechanisms remain unclear. To investigate this, potting experiments were conducted at three soil relative water content (SRWC): 75 +/- 5 % (W75), 60 +/- 5 % (W60), and 45 +/- 5 % (W45), along with K rates of 0 (K0), 150 (K150) and 300 (K300) kg K2O ha-1. Compared to W75, the W60 and W45 treatments reduced the photosynthesis of LSCBs in different FBs, adversely affecting carbohydrate accumulation in the subtending cotton bolls. K application can mitigate this negative impact, with the most pronounced effects observed in the middle and upper FBs. K application (K150 and K300) enhanced the net photosynthetic rate, stomatal conductance, maximum mass yield of PSII and chlorophyll content of LSCB in the middle and upper FBs compared to K0 under drought conditions. Additionally, K application significantly increased K content in LSCBs within the middle and upper FBs, which in turn elevated sucrose phosphate synthase (SPS), and sucrose synthase (SuSy) activities, reducing the conversion of sucrose into starch, ultimately facilitating carbohydrate exports to the subtending bolls. In summary, we propose a model that elucidates how K application mitigates drought damage by enhancing the exports of photoassimilates from the middle and upper FBs to their respective subtending cotton bolls.
These days, one of the main issues preventing agricultural development is salinized soils. Potassium fulvic acid (PFA) not only regulates plant growth, but also improves the soil nutrient content and physical structure, which makes it a soil conditioner worth promoting. Nevertheless, the research conducted thus far on the subject of PFA with regard to plant growth and inter-root microbial communities remains somewhat limited in scope. In this study, a pot experiment was conducted to simulate both the normal environment and salt stress environment. The objective of this experiment was to verify the effect of PFA on the growth of blueberry (Vaccinium corymbosum L.) as well as its effect on the soil physical and chemical indices and the soil microbial community structure. The findings demonstrated that the implementation of potassium fulvic acids exhibited a minimal impact on the growth of blueberry plants under standard environmental conditions. However, it was observed to exert a substantial effect on enhancing various physiological parameters, including plant height, root activity, and chlorophyll synthesis, particularly in response to salt stress. PFA led to a substantial augmentation in the soil organic matter content, alongside a notable rise in the alkali-hydrolyzable nitrogen (AN) and available potassium (AK) content. Concurrently, PFA caused a notable escalation in the activities of soil urease, sucrase, acid phosphatase, and catalase (p < 0.05) in the salt-stressed environment. PFA increased the abundance of Acidobacteria, Myxococcota, Ascomycota, and Fungi_phy_Incertae_sedis under salt stress, which was mainly related to the decrease in electrical conductivity (EC) values and increase in soil acid phosphatase (S-ACP) activity. It is evident that the implementation of PFA is advantageous in enhancing the saline environment, mitigating the impact of salt damage on blueberries and establishing a foundation for the expansion of cultivated areas and the sustainable cultivation of blueberries.
This study aimed to evaluate the effects of potassium (K) and Bradyrhizobium japonicum applications on physiological and microbial parameters in soybean plants under salt stress. The study included treatments of control, potassium (2.2 g K2SO4), bacteria (B), and their combinations (K + B), along with versions exposed to 100 mM NaCl salt stress. Key parameters such as leaf water content (RWC), chlorophyll (SPAD, Chlo a/b), oxidative stress indicators (H2O2 and MDA), proline, protein, antioxidant enzyme activities (APX, POD, and CAT), microbial biomass carbon (MBC), and CO2 release from soil were measured. Salt stress reduced RWC in plants by 15%, while H2O2 and MDA levels increased by 25% and 30%, respectively. However, potassium and bacterial applications improved plant resilience against stress by increasing proline levels by 20%, reducing protein loss by 18%, and enhancing antioxidant enzyme activities to mitigate oxidative damage. In soil microbial activities, MBC increased by up to 161%, and CO2 release increased by up to 27.7% with K + B application. Under salt stress, MBC and CO2 release were restored by 122% and 50.8%, respectively, demonstrating the positive effects of potassium and bacterial inoculation on microbial activity. These findings suggest that potassium and Bradyrhizobium japonicum applications could be considered effective strategies for enhancing plant tolerance and soil health under salt stress conditions.
The HKT protein family plays a vital role in plant responses to salt stress by mediating sodium (Na+) and potassium (K+) transport and maintaining Na+-K+ balance. Ipomoea pes-caprae (IPC), a pantropical creeping plant distributed along coastal regions in tropical and subtropical zones, exhibits exceptional salt tolerance. Understanding its salt tolerance mechanisms provides valuable insights for developing salt-tolerant crops and identifying candidate genes for genetic engineering. In this study, we identified two HKT genes, IpcHKT1;1 and IpcHKT1;2, in IPC. Phylogenetic analysis with HKT genes from other Ipomoea species revealed that all analyzed species contain two HKT genes located adjacently on the same chromosome. Comparative analysis of conserved motifs and intron-exon structures indicated that, despite their close evolutionary relationship, the HKT genes in IPC may exhibit functional divergence. Promoter analysis showed that their regulatory regions are enriched with cis-elements associated with responses to biotic and abiotic stresses, hormonal signaling, and growth, highlighting functional diversity within the HKT family. Subcellular localization experiments demonstrated that IpcHKT1;1 and IpcHKT1;2 are ion transporters localized to the plasma membrane. Heterologous expression in yeast confirmed their role in Na+/K+ symporter. Furthermore, RT-qPCR analysis revealed distinct expression patterns under salt stress: IpcHKT1;2 was significantly upregulated in roots, while IpcHKT1;1 expression was transitionally downregulated at 400 mM NaCl treatment. Prolonged high expression of IpcHKT1;2 in roots suggests its critical role in sustained salt stress tolerance. These findings provide new insights into the molecular mechanisms of salt tolerance in IPC. The identification of IpcHKT1;1 and IpcHKT1;2 as key players in salt stress responses offers promising genetic resources for enhancing crop resilience to soil salinity, addressing challenges associated with global salinization.
Global climate change accelerates the challenges of agricultural drought spells, which are alarming for food security and can trigger food scarcity. Therefore, improving soil-water retention capability and crop drought resilience is becoming more important for sustainable agriculture. This study investigates the individual and combined effects of biochar and potassium on soil water retention, crop drought resilience, and related physio-biochemical mechanisms over a 50-day growth period in potted plants. Pine needle biochar (350 g/10 Kg of soil) was used during the soil preparation stage while potassium sulfate (100 mg/L) was applied as a foliar spray at the development (10 days) and vegetative stages (45 days) under three drought stress conditions: control (100% FC), mild (75% FC) and severe (40% FC). The results revealed that the combined application of biochar and potassium significantly increased morphological, physiological, and biochemical attributes of maize plants under drought stress, improving shoot fresh weight by 11%, 6%, and 5%, root fresh weight by 19%, 19%, and 23%, shoot length by 17%, 16%, and 19%, and root length by 21%, 30%, and 29% under control, mild, and severe drought stress conditions, respectively. Similarly, relative water contents (RWC) increased by 12%, 16%, and 20%, water potential (Psi) increased by 26%, 22%, and 24%, osmotic potential (Psi s) increased by 100%, 59%, and 30%, and turgor potential (Psi p) increased by 28%, 35%, and 51% under combined treatment compared to control, mild, and severe drought stress. Additionally, biochar application with potassium foliar spray also improved membrane stability and integrity, cell wall loosening, membrane lipid peroxidation, and protein denaturing by decreasing electrolytic leakage by 35%, 28%, and 43%, proline by 30%, 27%, and 22%, hydrogen peroxidase by 47%, 45%, and 41%, and malondialdehyde contents by 24%, 20%, and 28% through activation of enzymatic (CAT, POD, SOD) and non-enzymatic (TSS, AsA, GSH) antioxidants. Furthermore, nutrient uptake was enhanced, with N increasing by 47%, 19%, and 45%, P by 64%, 82%, and 52%, and K by 24%, 42%, and 35% in shoots compared to normal, mild, and severe drought stress. These improvements mitigated cell dehydration, reduced transpiration inefficiency and delayed senescence, and ultimately supporting plant growth under drought stress. In conclusion, integrating biochar with potassium application effectively improves soil-water retention, alleviates oxidative stress and enhances drought tolerance in maize plants. This strategy can play a crucial role in sustainable agriculture by mitigating the adverse effects of drought stress and improving food security in drought-prone regions.
Phosphorus and potassium are essential macronutrients, and potassium dihydrogen phosphate, a compound containing both, plays a vital role in plant growth and reproduction. However, its rapid leaching poses significant environmental concerns, lessening its practical utility. To overcome this issue, a biodegradable hydrogel based on amla was synthesized through graft polymerization and evaluated as a water-retaining material for agricultural applications, specifically for the controlled release of fertilizers. The synthesized hydrogel was characterized using FTIR, SEM, XRD, and TGA. Its swelling properties, water retention capacity, porosity, and density were also examined. The biodegradable nature of the synthesized hydrogel was confirmed via soil burial and composting techniques, with FTIR used to validate the degradation. The hydrogel degraded almost entirely within 64 days in compost soil and 72 days in burial soil. Finally, potassium dihydrogen phosphate release studies were conducted, and the data were analyzed using Fick's law of diffusion and various kinetic models (zero order, first order, Higuchi, and Korsemers Peppas). The release pattern was measured via UV spectrophotometry over 45,000 min, demonstrating controlled nutrient delivery. These findings suggested that the synthesized hydrogel matrix has strong potential as an effective water retention system and for regulated nutrient release.
Polyhydroxybutyrate (PHB) has gained attention as an excellent packaging material due to its high crystallinity, biodegradability, low interaction with food matrices, and favorable mechanical properties. This study explores the development of PHB films incorporated with potassium sorbate (KS) and gallic acid (GA) via solvent casting, followed by a 30-day biodegradation test in soil. The films are analyzed for physicochemical and microbiological properties using X-ray diffraction, tensile testing, and disk diffusion assays. The soil-buried PHB films demonstrate accelerated biodegradation, likely driven by increased microbial and fungal activity, as well as moisture absorption. Incorporating KS and GA significantly enhances the antimicrobial efficacy of the films against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, with greater inhibition observed against S. aureus. This difference may stem from the additional lipopolysaccharide membrane in E. coli. Field emission scanning electron microscopy (FESEM) of the films, both pre- and post-biodegradation, provides further insights into their structural changes. These findings underscore the potential of PHB antimicrobial films in advancing sustainable food packaging applications.
Soil salinity is a major abiotic stress causing severe damage to plants. Thus, proper management approaches need to be developed to lessen the detrimental effect of salinity on crop growth and productivity. The objective of this study was to investigate the potential role of exogenous salicylic acid (SA) and potassium (K+) in mitigating the adverse effects of salt stress on tomato. Salt-stressed tomato seedlings Solanum lycopersicum L. cv. Agata were exposed to 0.1 mM SA and 5 mM K+, applied individually or simultaneously for two weeks. Obtained results showed that salt stress resulted in reduced growth rate associated with accumulation of Na+ ions, reduced K+ levels, lower K+/Na+ ratio, increased oxidative damage, reduced total chlorophyll and carbohydrate contents as well as disturbed proline accumulation and disrupted antioxidant system. Nevertheless, after SA and K+ supplementation, total chlorophyll, K+, total proteins, total carbohydrates, and proline contents as well as K+/Na+ ratio were significantly increased. Additionally, exogenous SA and K+ treatments enhanced the non-enzymatic and enzymatic antioxidant system and ensured better oxidative stress tolerance, as indicated by reduced H2O2 production and membrane lipid peroxidation, resulting in an increased membrane stability index. These effects were further enhanced by the simultaneous application of SA and K+, resulting in a better growth of salt-stressed tomato seedlings compared to single applications of these two growth regulators. Taken together, the results of the current study provide evidence that SA and K+ may interact to counteract the adverse effects of salt stress on the growth of tomato seedlings by improving osmotic and ionic homeostasis and upregulating the antioxidant defense system. Therefore, the simultaneous application of SA and K+ may be suggested as a promising approach for beneficial tomato growth at the seedling stage under salt-affected soil conditions.