This study was carried out to evaluate the interaction between terrestrial food crop plants and microplastics (MPs) with a focus on understanding their uptake, effects on growth, physiological, biochemical, and yield characteristics of two different cultivars of Solanum tuberosum L. i.e., Variety-1, Astrix (AL-4) and Variety-2, Harmes (WA-4). Polyethylene (PE), polystyrene (PS), and polypropylene (PP) spheres of size 5 mu m were applied to the soil at concentrations of 0 %, 1 %, and 5 %. Morphological parameters, including seed germination rate, shoot and root lengths, leaf area, and fresh and dry biomass of plants, got reduced significantly with the increase in MP concentration. PS MPs caused the most negative impact, particularly at 5 %, leading to the greatest decline in growth and Na, Mg, Zn, Cu, Ni, and Mn nutrient content. The highest DPPH scavenging activity was observed in the 5 % PS MPs treatment with approximately a 45.34 % increase from the control, indicating its potential to enhance antioxidant activity in response to stress caused by PS MPs. Both reducing and non-reducing sugar contents and total proteins were also decreased significantly. Vitamin C content exhibited a significant increase in response to MPs, with the highest levels recorded under 5 % PS MPs treatments. This suggests an adaptive antioxidant response to mitigate oxidative damage induced by MPs. SEM analysis revealed tissue infiltration of MP particles in shoots, leaves, and tubers of both varieties. Among MPs, PS had the most detrimental effects, followed by PP and PE, with higher concentrations increasing the negative impact.

As emerging pollutants, microplastics (MPs) pose serious threats to the terrestrial ecosystems, and the long-term presence of aged MPs in soil results in toxic effects on plant growth. However, the phytotoxicity mechanisms of aged MPs remain unclear. To understand the toxic effects of aged MPs and the response mechanism of lettuce plants, we selected polyethylene (PE) and polypropylene (PP) (commonly found in soil), and then studied the effects of the two phytotoxins on the soil-plant system before and after aging of the MPs. We found that aging enhanced the toxicity of the MPs to the plants. Compared with the original MPs-treatment group, aged PE and PP particles reduced plant biomasses by 26.19%-28.44% and 25.58%-26.13%, respectively, potentially due to the effects of aged MPs on the rhizosphere soil, which further inhibited nutrient absorption in lettuce. The metabolic response of lettuce to MPs was also different. Aged PE significantly attenuated malic acid and proline concentrations in lettuce, and the reduction in these two products inhibited photosynthesis, energy metabolism, and cellular homeostasis, thereby aggravating the damage caused by aged PE. Aged PP principally affected the metabolic pathways of phenylalanine, tyrosine and tryptophan, which was postulated to be the reason why aging enhanced the phytotoxicity of PP. This study provides new insights into the assessment of the toxic effects of MPs, as well as the environmental behavior and ecological risks of aged MPs.

Traditional soil stabilization methods, including cement and chemical grouting, are energy-intensive and environmentally harmful. Microbial-induced carbonate precipitation (MICP) technology offers a sustainable alternative by utilizing microorganisms to precipitate calcium carbonate, binding soil particles to improve mechanical properties. However, the application of MICP technology in soil stabilization still faces certain challenges. First, the mineralization efficiency of microorganisms needs to be improved to optimize the uniformity and stability of carbonate precipitation, thereby enhancing the effectiveness of soil stabilization. Second, MICP-treated soil generally exhibits high fracture brittleness, which may limit its practical engineering applications. Therefore, improving microbial mineralization efficiency and enhancing the ductility and overall integrity of stabilized soil remain key issues that need to be addressed for the broader application of MICP technology. This study addresses these challenges by optimizing microbial culture conditions and incorporating polyethylene fiber reinforcement. The experiments utilized sandy soil and polyethylene fibers, with Bacillus pasteurii as the microbial strain. The overall experimental process included microbial cultivation, specimen solidification, and performance testing. Optimization experiments for microbial culture conditions indicated that the optimal urea concentration was 0.5 mol/L and the optimal pH was 9, significantly enhancing microbial growth and urease activity, thereby improving calcium carbonate production efficiency. Specimens with different fiber contents (0% to 1%) were prepared using a stepwise intermittent grouting technique to form cylindrical samples. Performance test results indicated that at a fiber content of 0.6%, the unconfined compressive strength (UCS) increased by 80%, while at a fiber content of 0.4%, the permeability coefficient reached its minimum value (5.83 x 10-5 cm/s). Furthermore, microscopic analyses, including X-ray diffraction (XRD) and scanning electron microscopy with energy-dispersive spectroscopy (SEM-EDS), revealed the synergistic effect between calcite precipitation and fiber reinforcement. The combined use of MICP and fiber reinforcement presents an eco-friendly and efficient strategy for soil stabilization, with significant potential for geotechnical engineering applications.

An emerging alternative to improve the mechanical properties of fine soils susceptible to cracking is the addition of fibers obtained from reused synthetic materials such as polyethylene terephthalate (PET). The technical literature on the fracture mechanics of PET fiber-reinforced soils is rather scarce, so there has been insufficient progress in determining fracture parameters and standardized procedures to find optimal reinforcement conditions. This research uses experimental techniques to induce tensile stresses in clayey silty soil samples from the Valley of Mexico reinforced with different fiber contents. By applying approaches based on linear elastic and elastoplastic theory, parameters useful for the study of fracture mechanics and flexural strength of PET- reinforced soil were estimated: tensile strength, critical energy release rate, critical stress intensity factor, and contour integral for crack propagation under plasticity. In addition, imaging techniques are used to measure the deformations generated in bending tests of reinforced soil beams and to study crack propagation from initiation to maximum stresses. The addition of PET fibers significantly improved soil response by reducing cracking, increasing tensile strength, and providing ductile behavior as cracking progressed. These effects indicate the great potential of recycled PET fibers as a subgrade improvement method for soft, cracking soil deposits, or even for earthworks and slope stabilization in clayey soils on road projects.

PurposeThe present work aims to prepare biocomposites blend based on linear low density polyethylene/ starch without using harmful chemicals to improve the adhesion between two phases. Also, the efficiency of essential oils as green plasticizers and natural antimicrobial agents were evaluated.Design/methodology/approachBarrier properties and biodegradation behavior of linear low density polyethylene/starch (LLDPE/starch) blends plasticized with different essential oils including moringa oleifera and castor oils wereassessed as a comparison with traditional plasticizer such as glycerol. Biodegradation behavior forLLDPE/starch blends was monitored by soil burial test. The composted samples were recovered then washed followed by drying, and weighting samples after 30, 60, and 90 days to assess the change in weight loss. Also, mechanical properties including retention values of tensile strength and elongation at break were measured before and after composting. Furthermore, scanning electron microscope (SEM) was used to evaluate the change in the morphology of the polymeric blends. In addition to, the antimicrobial activity of plasticized LLDPE/starch blends films was evaluated using a standard plate counting technique.FindingsThe results illustrate that the water vapor transition rate increases from 2.5 g m-2 24 h-1 for LLDPE/5starch to 4.21 g m-2 24 h-1 and 4.43 g m-2 24 h-1 for castor and moringa oleifera respectively. Also, the retained tensile strength values of all blends decrease gradually with increasing composting period. Unplasticized LLDPE/5starch showed highest tensile strength retention of 91.6% compared to the other blends that were 89.61, 88.49 and 86.91 for the plasticized LLDPE/5starch with glycerol, castor and M. oleifera oils respectively. As well as, the presence of essential oils in LLDPE/ starch blends increase the inhibition growth of escherichia coli, candida albicans and staphylococcus aureus.Originality/valueThe objective of this work is to develop cost-effective and environmentally-friendly methods for preparing biodegradable polymers suitable for packaging applications.

Using tapes in drip irrigation is associated with environmental problems due to the accumulation of tapes in agricultural areas. Farmers either leave them on the fields or burn them or bury them. All three of these methods pose dangerous environmental hazards. To address this issue, it is recommended that these materials be produced from or with biodegradable materials. In this study, a biodegradable additive was used as a degradation accelerator in the production of tapes. After the production of these tapes, they were used under real conditions and during a growing season and in two treatments: below and on the soil surface, along with a canopy and without shade (beans and radishes). After 6 and 11 months, the tapes were sampled to investigate their degradation. The results showed that tapes made with oxo as an additive began to degrade more quickly than did conventional tapes. A reduction in properties such as weight (p 0.05), Young's modulus (p < 0.05) and toughness (p < 0.05) in tapes produced with oxo additives shows more and faster degradation than conventional tapes. Therefore, the use of oxo master batches in the production of tapes is possible and useful.

This study aims to investigate the biodegradation potential of a gut bacterial strain, Bacillus cereus AP-01, isolated from Tenebrio molitor larvae fed Styrofoam, focusing on its efficacy in degrading low-density polyethylene (LDPE). The biodegradation process was evaluated through a series of assays, including clear zone assays, biodegradation assays, and planktonic cell growth assessments in mineral salt medium (MSM) over a 28-day incubation period. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were employed to characterize the alterations in LDPE pellets, followed by molecular characterization. Over three months, sterile soil + LDPE pellets were treated with different concentrations of gut bacterial strain. The degradation capabilities were assessed by measuring pH, total microbial counts, carbon dioxide evolution, weight loss, and conducting phase contrast microscopy and mechanical strength tests. Results demonstrated that MSM containing LDPE as a carbon source with gut bacterial strain produced a clear zone and enhanced planktonic cell growth. FTIR analysis revealed the formation of new functional groups in the LDPE, while SEM images displayed surface erosion and cracking, providing visual evidence of biodegradation. Molecular characterization confirmed the strain as Bacillus cereus AP-01 (NCBI Accession Number: OR288218.1). A 10% inoculum concentration of Bacillus cereus AP-01 exhibited increased soil bacterial counts, carbon dioxide evolution, and pH levels, alongside a notable weight loss of 30.3% in LDPE pellets. Mechanical strength assessments indicated substantial reductions in tensile strength (7.81 +/- 0.84 MPa), compression (4.92 +/- 0.53 MPa), hardness (51.96 +/- 5.62 shore D), flexibility (10.62 +/- 1.15 MPa), and impact resistance (14.79 +/- 0.94 J). These findings underscore the biodegradation potential of Bacillus cereus AP-01, presenting a promising strategy for addressing the global LDPE pollution crisis.

The high global production combined with low recycling rates of polystyrene (PS) and low-density polyethylene (LDPE) contributes to the abundance of these commonly used plastics in soil, including as microplastics (MPs). However, the combined effects of MPs and heavy metals, such as arsenic (As) on earthworms are poorly understood. Here, we show that neither PS nor LDPE altered the effects of As on the survival, growth, and reproduction of the earthworm Eisenia fetida. As stress, both alone and in combination with the MPs, induced DNA damage in coelomocytes. In As-exposed earthworms, PS and LDPE increased the accumulation of reactive oxygen species while the activities of the antioxidant enzymes peroxidase, superoxide dismutase, and catalase were significantly lower under combined PS/LDPE + As exposure than under As exposure alone. As stress alone reduced cocoon production and the mRNA level of the reproduction-related gene ANN whereas As combined with PS/LDPE reduced the mRNA levels of CYP450, an enzyme involved in detoxification. Integrated biomarker response analysis revealed that PS/LDPE did not significantly impact the overall ecotoxicological effects of As exposure on earthworms. This study provides important insights into the potential ecological risks of MPs in heavy-metal-contaminated soil.

Cellulose crystallinity can be altered by various treatment methods, including mechanical or chemical treatments, which can affect the properties of thermoplastic composites. In this study, the crystallinity of cellulose was manipulated using mechanical ball milling. The primary objective was to assess the impact of altering the cellulose crystallinity on the overall performance of high-density polyethylene (HDPE)-based composites. The mechanical and structural properties of the composites were assessed using tensile and impact tests, attenuated total reflectance infrared ( ATR-IR ) spectroscopy, scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). The degradation properties of the HDPE composites were evaluated using a soil-burial degradation test. The impact of cellulose crystallinity on the mechanical properties of HDPE composites showed a marginal enhancement of 5% in tensile strength with the incorporation of 2% low-crystallinity cellulose (LCC). The highest impact strength of the HDPE composites was attained by the incorporation of 6% LCC. ATR-IR analysis showed that the peak intensity of the HDPE-LCC composite decreased, whereas the HDPE composite with high-crystallinity cellulose (HCC) did not exhibit changes in peak intensity compared to the HDPE spectrum. SEM examination showed that LCC possessed superior dispersion in the HDPE matrix compared to that of HCC. Thermal degradation decreased by up to 32% with the addition of HCC and LCC. A soil burial degradation study showed that the mechanical properties of the HDPE-LCC composite deteriorated more than those of the HDPE-HCC composite after 24 months. This study concluded that altering the crystallinity of cellulose can lead to composites with tailored properties.

Lignosulfonate (LS), an environmentally friendly and non-toxic material, has attracted attention as a non-traditional soil stabilizer. However, LS could be easily washed out from soil due to its high water-solubility, which leads to the consequent loss of strength. Therefore, an additional admixture is needed to overcome this limitation. In this study, polyethyleneimine (PEI) was mixed with LS to stabilize silica sand. The consequent improvements in the water-resistant and strength characteristics of LS-treated soil were investigated through the unconfined compressive strength (UCS) test, triaxial test, and cyclic wetting- drying tests. The results demonstrated that the UCS had an increasing trend with a rise in LS content. Moreover, the UCS was influenced by the drying out of the water from the specimen related to the LS concentration and the curing time: a higher concentration and a longer curing duration improve the UCS. According to the triaxial test, the deviatoric stress also increased with the LS content. In addition, both the soil's cohesion and secant elastic modulus were improved in a more ductile manner than typical cemented soil. In the cyclic wetting-drying test, no disintegration of the specimen was observed. Although the UCS of the treated soil in wet condition revealed a notable decrease, after re-dry for seven days in a controlled room, its strength recovered to about 86% of that in its initial dry condition.