In today's highly competitive and interconnected global market, economic achievement and prosperity are essential needs for every individual. However, in recent years, the science of sustainability has gained popularity due to mounting evidence of the damaging impacts of environmental issues. Lately, the expansion of petroleum industries and refineries has led to a substantial rise in the production of refinery oily waste. The treatment of such waste presents significant environmental challenges, necessitating the development of sustainable solutions. This review explores the latest advancements in biological processes for treating it, focusing on their efficacy and limitations. These processes are still facing challenges such as slow degradation rates, nutrient availability, and pollutant toxicity, which can hinder efficiency. To address these, efforts are being made to develop more viable biological treatments including exploration of microbial strains, optimizing process conditions, bioreactor systems, and integrating advanced bioremediation techniques. Potential applications of these processes across different contaminated sites are discussed along with commercially available technologies. Drawbacks related to bioprocess scale-up, cost-effectiveness, and regulatory constraints are also addressed. Additionally, it incorporates pertinent case studies that serve as illustrations of successful implementations of biological strategies. Ultimately, this sets the stage for practical bioremediation implementation as a solution for refinery waste management.

Environmental context Mitigating the environmental fallout of industrial accidents is crucial. In a recent study, researchers conducted tests on model substrates to explore the effectiveness of bioremediation in treating complex refinery contaminants resulting from both accidental and deliberate facility damage. The research reveals that bioremediation can be a promising, eco-friendly solution for cleaning up such pollutants, aligning with broader efforts to combat environmental harm resulting from industrial incidents.Rationale Bioremediation harnesses microorganisms' diverse metabolic abilities to detoxify and eliminate pollutants, particularly hydrocarbon-based ones such as oil. This natural biodegradation process performed by microorganisms is a cost-effective method for environmental cleanup compared to other remediation technologies.Methodology In this study, we examined the fate of heavy metals, cobalt and molybdenum, by the analysis of the basic chemical parameters of other sample components, such as n-hexane extractable substances and total petroleum hydrocarbons. The metal content was determined using inductively coupled plasma-optical emission spectrometry (ICP-OES). Exchangeable (loosely bound to the surface of particles and due to its high mobility and availability is crucial for understanding the potential immediate impact of metal contamination) and more stable fractions of the metal and the metal forms were determined using a sequential extraction method. The phase composition of the samples was determined by X-ray diffraction.Results In our microbiological analysis, we isolated various cultures from a consortium of microorganisms. Basic chemical analysis indicators, such as n-hexane extractable substances, total petroleum hydrocarbons and humic acids, reflected robust microbiological activity. During the study, metals in exchangeable form decreased and those in more stable forms increased.Discussion The sequential extraction of cobalt and molybdenum revealed shifts in various metal fractions within the bioaugmented substrate post-bioremediation, differing from the initial substrate. These alterations in metal fractions are likely attributable to microbial actions, leading to the formation of more stable metal fractions throughout the bioremediation process.