Atrazine (ATR), a widely used herbicide, poses significant environmental and health risks due to its high solubility and adsorption in soil. ATR exposure can lead to nephrotoxicity in humans and animals. Curcumin (Cur), an active compound in Curcuma species, is renowned for its antioxidant and anti-inflammatory properties, with potential to mitigate chronic disease risks. We hypothesized that the addition of Cur could alleviate renal impairment associated with ATR exposure and carried out experiments using mice as subjects. This study investigates whether Cur can attenuate ATR-induced nephrotoxicity in mice by modulating mitophagy and apoptotic pathways. Our findings illustrate that consumption with Cur attenuates nephrotoxicity induced by ATR, as evidenced by lowered serum concentrations of uric acid (UA), blood urea nitrogen (BUN), and creatinine (CRE), established biomarkers of renal injury. Moreover, Curcumin enhances renal antioxidant defense mechanisms in ATR-exposed mice, as indicated by elevated levels of total antioxidant capacity (T-AOC), catalase (CAT), and glutathione peroxidase (GSH-Px), alongside reduced levels of malondialdehyde (MDA). Histopathological and electron microscopy analyses further corroborate these findings, showing reduced organelle damage, particularly mitochondrial ridge breakage and vacuolization, and increased autophagic lysosomes. Cur further enhances PINK1/Parkin-mediated autophagy, as evidenced by elevated levels of PINK1, Parkin, LC3BII, and P62 compared to ATR-treated mice. Moreover, Cur mitigates the mitochondrial apoptotic pathway, indicated by the down-regulation of apoptosis-related genes (Cytochrome C (Cyto-C), Caspase3, Caspase9) and the proapoptotic marker (Bax), along with the up-regulation of the anti-apoptotic marker (Bcl-2) at both transcriptional and translational levels compared to ATR-treated mice. In summary, Cur demonstrates nephroprotective properties against ATR-induced injury through the enhancement of mitochondrial autophagy and display of antiapoptotic actions, underscoring its curative potency as a treatment for nephrotoxicity caused by ATR.

Background: Pesticide residues can cause chronic toxicity to the human body and lead to a series of diseases that damage the liver. Therefore, developing a highly sensitive, selective, and low-cost pesticide residues detection method is of great significance for protecting human health and safety. Nowadays, commonly used methods for pesticide residue detection include gas chromatography, high-performance liquid chromatography, and fluorescence sensing. These methods have some typical shortcomings, such as long sample pretreatment time, expensive instruments, and poor controllability. It was thought that a sensing platform based on electrochemical analysis method and functional DNA molecules can eliminate the above drawbacks. Results: Herein, this study developed a simple and label-free electrochemical aptasensor based on a triple- stranded DNA molecular switch. Acetamiprid (ACE) was served as the analytical model, and its binding with the aptamer opened the triple-stranded DNA molecular switch, resulting in the in-situ formation of G-quadruplex/hemin complexes on the electrode surface, obtaining a significantly enhanced electrochemical signal and achieving high specificity and label-free detection of ACE, with a detection limit as low as 4.67 x 10-3 nM (S/N = 3). In addition, due to the specific recognition between the aptamer and the target, the aptasensor effectively avoided the interference of other pesticides and exhibited good specificity. Moreover, benefiting from the pH switchable of the triple-stranded DNA molecular switch and the programmability of DNA molecules, OR logic gate and OR-INHIBIT cascade logic circuit were successfully implemented. Significance: The proposed electrochemical aptasensor exhibited good accuracy and sensitivity in detecting acetamiprid in vegetable soil sample, indicating its practicality in the detection of pesticide residues in actual samples. Furthermore, the sensing system was reasonably programmed and successfully operated an OR logic gate and an OR-INHIBIT cascade logic circuit, demonstrating its potential application in intelligent sensing.

Non-degradable plastic mulch films used in agriculture are polluting the environment by leaving residues and microplastics in the soil. They are also difficult to recycle due to contamination during their use. Biodegradable mulch films are needed as alternatives so that they can be used effectively during the growing season and later be ploughed to be degraded in soil. However, market-available so-called biodegradable mulch films are very slow to degrade in the natural environment and thus do not fit with crop rotation demands or annual cultivation. In this study, we have developed mulch films from cotton gin trash (CGT) and/or gin motes (GM) in combination with biodegradable polycaprolactone and demonstrated their effectiveness over 3 months in outdoor conditions. Both the stability and degradation behaviours of mulch film samples were observed when they were placed on top of the soil and buried in the soil, respectively. Pesticide residue analysis also was carried out on CGT powder to identify and quantify individual pesticides against a matrix of known pesticides. The mulch films prepared in this study showed comparable and stable mechanical properties compared to commercial biodegradable mulch film, though were much quicker to degrade when buried in the soil. No pesticides were detected in the CGT samples. The films produced were vapour-permeable and may be useful in practical agricultural settings by being able to maintain consistent soil moisture and allowing precipitation to penetrate gradually. The lab-scale productioncost for the film was 98.8 AUD/kg, which could be lowered by integrating a continuous film line in large-scale production.

Pesticides serve a crucial function in contemporary farming practices, safeguarding agricultural crops against pest infestations and boosting production outputs. However, indiscriminate use has caused environmental and human health damage. This study aimed to develop and validate a gas chromatography-flame ionization detection (GC-FID) methodology for the direct and routine analysis of spiromesifen residues in soil, leaves, and tomato fruits. The proposed method prioritizes simplicity by avoiding derivatization steps, offering advantages over existing approaches that utilize lengthy multi-step extraction or derivatization prior to GC analysis. A key novelty of this work is the development of a QuEChERS extraction coupled directly to GC-FID without further clean-up or chemical treatment steps, rendering the method more convenient and accessible for routine monitoring applications. Factors evaluated included: sample solvent; inlet and column temperature profiles; inlet type; sample volume; and injection technique. Recovery and matrix effect studies were conducted by fortifying tomato, leaf, and soil matrices at three different concentrations (0.5, 1, and 10 mu g ml(-1)). Quadruplicate analyses (n = 4) yielded mean recoveries of 98.74% (fruits), 93.92% (leaves), and 94.18% (soil), confirming efficient extraction. Matrix effects were negligible at -7.9%,-7.8%, and -5.3%, respectively. The chromatographic linearity of the developed GC-FID method was excellent over the 0.002-20 mu g ml(-1) range with R-2 > 0.9979. The method demonstrated good precision, with inter- and intra-day RSD% ranging from 0.06-1.8%, below the 3% limit. GC-MS analysis confirmed spiromesifen identification. Under greenhouse conditions, residual levels were 1.39 mg/kg in soil, 8.24 mg/kg in tomato, and 3.39 mg/kg in leaves. Dissipation followed first-order kinetics with a half-life of 1.6 days. The optimized GC-FID method is promising for monitoring spiromesifen usage and guiding agricultural practices.

This study delved into the impact of open biomass burning on the distribution of pesticide and polycyclic aromatic hydrocarbon (PAH) residues across soil, rice straw, total suspended particulates (TSP), particulate matter with aerodynamic diameter <= 10 mu m (PM10), and aerosols. A combination of herbicides atrazine (ATZ) and diuron (DIU), fungicide carbendazim (CBD), and insecticide chlorpyriphos (CPF) was applied to biomass before burning. Post-burning, the primary soil pesticide shifted from propyzamide (67.6%) to chlorpyriphos (94.8%). Raw straw biomass retained residues from all pesticide groups, with chlorpyriphos notably dominating (79.7%). Ash residue analysis unveiled significant alterations, with elevated concentrations of chlorpyriphos and terbuthylazine, alongside the emergence of atrazine-desethyl and triadimenol. Pre-burning TSP analysis identified 15 pesticides, with linuron as the primary compound (51.8%). Post-burning, all 21 pesticides were detected, showing significant increases in metobromuron, atrazine-desethyl, and cyanazine concentrations. PM10 composition mirrored TSP but exhibited additional compounds and heightened concentrations, particularly for atrazine, linuron, and cyanazine. Aerosol analysis post-burning indicated a substantial 39.2-fold increase in atrazine concentration, accompanied by the presence of sebuthylazine, formothion, and propyzamide. Carcinogenic PAHs exhibited noteworthy post-burning increases, contributing around 90.1 and 86.9% of all detected PAHs in TSP and PM10, respectively. These insights advance understanding of pesticide dynamics in burning processes, crucial for implementing sustainable agricultural practices and safeguarding environmental and human health.