Polystyrene nanoplastics (PS-NPs) have been demonstrated to accumulate in organisms especially from soil and exhibit neurotoxicity. However, the specific mechanisms by which PS-NPs caused neurotoxic effects remain largely unexplored. In this study, we employed PS-NPs with a diameter of 50 nm as the toxicant and used estimated exposure concentrations which are similar to those found in Chinese agricultural soil (i.e., 0, 1, 5 and 10 mu g/mL). We found that PS-NPs induced significant neurotoxicity and behavioral damage in nematodes. Taking advantage of neuronal-specific reporter nematodes, we unveiled the order of neuronal damage induced by PSNPs being DAergic neurons, followed by Achergic neurons and GABAergic neurons. Additionally, PS-NPs significantly reduced the neurotransmitter levels corresponding to these three types of neurons, with the order of reduction being Ach followed by DA and GABA. Moreover, we demonstrated that PS-NPs led to an increase in ROS production, the activation of gst-4 and a decrease in Sod-2 protein content. Furthermore, we unveiled that Sod-2 could suppress the generation of ROS induced by PS-NPs. Then we proved that the pretreatment with mitochondrial ROS scavenger Mitoquinone (Mito Q) was able to alleviate PS-NPs-induced neurotoxic effects and behavioral damage by scavenging ROS and subsequently regulating Sod-2 protein expression. In summary, we have demonstrated for the first time that ROS-mediated reduction of Sod-2 protein plays a crucial role in PS-NPsinduced neurotoxicity and behavioral damage. Furthermore, Mito Q shows potential therapeutic value in alleviating the toxic effects of PS-NPs, providing new insights for the prevention and treatment of PS-NPs-induced neurotoxicity.

Vascular wilt is an important tomato disease that affects culture yields worldwide, with Fusarium oxysporum (F.o) being the causal agent of this infection. Several management strategies have lost effectiveness due to the ability of this pathogen to persist in soil and its progress in vascular tissues. However, nowadays, research has focused on understanding the plant defense mechanisms to cope with plant diseases. One recent and promising approach is the use of extracellular DNA (eDNA) based on the ability of plants to detect their self-eDNA as damage-associated molecular patterns (DAMPs) and pathogens' (non-self) eDNA as pathogen-associated molecular patterns (PAMPs). The aim of this work was to evaluate the effect of the eDNA of F.o (as a DAMP for the fungus and a PAMP for tomato plants) applied on soil, and of tomato's eDNA (as a DAMP of tomato plants) sprayed onto tomato plants, to cope with the disease. Our results suggested that applications of the eDNA of F.o (500 ng/mu L) as a DAMP for this pathogen in soil offered an alternative for the management of the disease, displaying significantly lower disease severity levels in tomato, increasing the content of some phenylpropanoids, and positively regulating the expression of some defense genes. Thus, the eDNA of F.o applied in soil was shown to be an interesting strategy to be further evaluated as a new element within the integrated management of vascular wilt in tomato.

Cortaderia selloana (Schult. & Schult. f.) Asch. & Graebn. (Pampas grass) is a perennial grass native to temperate and subtropical regions of South America. The species was introduced to western Europe for ornamental purposes during the nineteenth century, where it has become naturalized in anthropogenic and natural habitats, especially in sandy, open, and disturbed areas. Female plants of C. selloana produce thousands of seeds that are dispersed over long distances by wind and germinate readily. Its invasive success is also attributed to its ability to adapt and tolerate a wide range of environmental conditions, such as high salinity levels, long droughts, and soil chemical pollution. Cortaderia selloana usually invades human-disturbed habitats where it encounters little competition with other plants and high resource availability. However, the species can invade natural habitats, especially those with high light availability, causing biodiversity loss and changes in ecosystem functioning (e.g. alteration of succession and nutrient dynamics). The species may cause negative socio-economic impacts by reducing productivity of tree plantations, causing respiratory allergies, and decreasing the recreational value of invaded areas. Control costs are high due to the extensive root system that C. selloana develops and the high resprouting ability following physical damage. Although herbicides are effective control measures, their use is not allowed or is undesirable in all situations where the plant occurs (e.g. near riverbanks, natural protected sites). No biological control agents have been released on C. selloana to date, but the planthopper Sacchasydne subandina and the gall midge Spanolepis selloanae are promising targets.

Inner dumps formed by mining activities were prone to soil erosion. Slope improvement is an effective means to control soil erosion and ecological restoration. However, little research has been done on the effect of improver application methods on the effectiveness of amendments. This paper combined similar theory to build an indoor inner dump model, used simulated rainfall tests and microstructural analysis to investigate the erosion resistance and water retention of the inner dump under different methods of PAM. The results showed thattrace source erosion and side erosion didn't occur in the inner dump model without PAM applied. Spreading PAM powder granules and spraying PAM gum liquid could reduce the internal earth pressure and pore water pressure of the inner dump to rainfall. Spraying PAM gum liquid could form a uniform film that can more effectively prevent rainfall infiltration. The carboxyl groups generated by the reaction of PAM and water could enhance the adhesion of clay minerals in the inner dump. The hydrogen bonds in the PAM gum liquid could connect water molecules, thereby forming interlocking soil particle aggregates, which enhance the anti-erosion ability of the inner dump. Spreading PAM powder granules and spraying PAM gum liquid can reduce the porosity of the improved soil, but spraying PAM gum liquid could reduce the porosity by 56.44%, which can effectively reduce water loss and had the best water retention. Therefore, spraying PAM gum liquid had better anti-erosion ability, which could lay the foundation for the ecological restoration of the inner dump.

This research aimed to examine the compound effects of polyacrylamide (PAM) and cadmium (Cd) on plant growth subsequent to the mixing of municipal sludge with soil and to explore the potential ecological risks associated with the use of sludge. A pot experiment was performed wherein four gradients (0, 5, 15, and 20 g/[kg dry sludge]) were established corresponding to different PAM concentrations to assess the effects on the physiological and biochemical parameters of Brassica campestris L. ssp. chinensis Makino and Brassica campestris L. and the effects on Cd and PAM concentrations in soil. The findings indicated that the biomass of both plants increased when the total PAM concentration ranged from 5 g to 15 g/(kg dry sludge). Concurrently, polyacrylamide (PAM) downplayed the uptake of Cd by the plants, reduced phytotoxicity, and increased the activities of antioxidant enzymes such as superoxide dismutase (SOD) and peroxidase (POD) in the roots. However, the biomass of Brassica campestris L. ssp. chinensis Makino and Brassica campestris L. decreased significantly when the total PAM concentration was exceeded 15 g/(kg dry sludge), and the toxic effect of Cd on pants was enhanced to some extent. Especially when the PAM concentration was 20 g/(kg dry sludge), apparent cell damage was observed in root cells. It was further noted that Brassica campestris L. portrayed a higher tolerance towards higher proportions of sludge compared with Brassica campestris L. ssp. chinensis Makino. Sludge holds a wider scale of applicability in landscaping than in agriculture. A fusion of polyacrylamide (PAM) and antioxidant enzymes could potentially counteract the effects of Cd within a specific concentration range, serving as a defense mechanism against stress. The data obtained from this study are crucial for unraveling anti-stress responses and detoxification mechanisms employed by different plant species under the influence of factors such as PAM, Cd, and others, thus providing novel insights into research related to land use involving sludges.

Freeze-thaw (FT) alternation can severely affect the properties of fine-grained soils, often leading to the failure of foundation backfill projects, such as open canals and roads in cold regions. To address these problems, a water-soluble anionic polyacrylamide (APAM) was used to improve the properties of fine-grained soils, particularly silty clay. This study aims to analyze the effects of the APAM polymer on the physico-mechanical and microstructural properties of silty clay under both unfrozen and FT cycles. For this purpose, different addition dosages and FT cycle numbers were determined, and then a series of zeta potential, thermal conductivity, permeability, triaxial compression, and scanning electron microscopy (SEM) tests were conducted on the soil samples. Additionally, a unidirectional freezing test was constructed to investigate the coupled thermo-hydro-mechanical processes of APAM-treated soil samples. The results showed that the addition of the APAM polymer significantly enhanced the macroscopic engineering properties and microstructural characteristics of silty clay. This improvement was attributed to the charge neutralization, adsorption bridging effect, and hydrophobic interaction provided by the APAM polymer. However, all the soil samples showed a significant deterioration in their engineering properties during FT cycles, especially after the third FT cycle. It was notable that APAM-treated soil samples were superior to the untreated sample in terms of FT resistance. During the unidirectional freezing process, the pore water migrated from the unfrozen zone towards the freezing front due to the temperature gradient. The treated sample's pore water migration volume was considerably lower than that of the untreated sample, resulting in a 55.25% reduction in total frost heave when the APAM dosage was 0.30%. The findings of this paper may be utilized to mitigate the risk of frost damage to foundation backfill projects in cold regions, as well as to get a better understanding of the stabilization mechanism of the eco-friendly APAM polymer.

The Vanj River Basin contains a dynamic glacier, the Medvezhiy glacier, which occasionally poses a danger to local residents due to its surging, flooding, and frequent blockages of the Abdukahor River, leading to intense glacial lake outburst floods (GLOF). This study offers a new perspective on the quantitative assessment of glacier surface velocities and associated lake changes during six surges from 1968 to 2023 by using time-series imagery (Corona, Hexagon, Landsat), SRTM elevation maps, ITS_LIVE, unmanned aerial vehicles, local climate, and glacier surface elevation changes. Six turbulent periods (1968, 1973, 1977, 1989-1990, 2001, and 2011) were investigated, each lasting three years within a 10-11-year cycle. During inactive phases, a reduction in the thickness of the glacier tongue in the ablation zone occurred. During a surge in 2011, the flow accelerated, creating an ice dam and conditions for GLOF. Using these datasets, we reconstructed the process of the Medvezhiy glacier surge with high detail and identified a clear signal of uplift in the surface above the lower glacier tongue as well as a uniform increase in velocities associated with the onset of the surge. The increased activity of the Medvezhiy glacier and seasonal fluctuations in surface runoff are closely linked to climatic factors throughout the surge phase, and recent UAV observations indicate the absence of GLOFs in the glacier's channel. Comprehending the processes of glacier movements and related changes at a regional level is crucial for implementing more proactive measures and identifying appropriate strategies for mitigation.

Mixing state of black carbon (BC) with secondary species has been highlighted as a major uncertainty in assessing its radiative forcing. While recent laboratory simulation has demonstrated that BC could serve as a catalyst to enhance the formation of sulfate, its role in the formation and evolution of secondary aerosols in the real atmosphere remains poorly understood. In the present study, the mixing of BC with sulfate/nitrate in the atmosphere of Guangzhou (China) was directly investigated with a single particle aerosol mass spectrometer (SPAMS). The peak area ratios of sulfate to nitrate (SNRs) for the BC-containing particles are constantly higher than those of the BC-free particles (defined as particles with negligible BC signals). Furthermore, the seasonal SNR peak is observed in summer and autumn, and the diurnal peak is found in the afternoon, consistent with the trends of radiation-related parameters (i.e., solar radiation and temperature), pointing to the BC-induced photochemical production of sulfate. Such hypothesis is further supported by the multilinear regression and random forest analysis, showing that the variation of SNRs associated with the BC-containing particles could be well explained (R-2 = similar to 0.7-0.8) by the radiation-related parameters (>30% of the variance) and the relative BC content (similar to 20%) in individual particles, but with limited influence of precursors (SO2/NOx: <5%). Differently, the radiation-related factors only explain <10% of the SNR variation for the BC-free particles. These results provide ambient observational evidence pointing to a unique role of BC on the photochemical formation and evolution of sulfate, which merits further quantitative evaluations.

Portable aethalometers are commonly used for online measurements of light-absorbing carbonaceous particles (LAC). However, they require strict calibration. In this study, the performance of a micro-aethalometer (MA200 with polytetrafluoroethylene filter) in charactering brown carbon aerosol (BrC) absorption was evaluated in comparison with reference materials and techniques that included bulk solution absorbance and Mie-theory based particle extinction retrieval via broadband cavity enhanced spectrometer (BBCES). Continuous-wavelength resolved (300-650 nm) imaginary refractive index (k(BrC)) was derived with these methods for various BrC proxies and standard materials representing a wide range of sources and absorbing abilities, including the strongly absorbing nigrosin, pahokee peat fluvic acid (PPFA), tar aerosol from wood pyrolysis, humic-like substance (HULIS) separated from wood smoldering burning emissions, and secondary organic aerosols (SOA) from photochemical oxidation of indole and naphthalene in the presence of NOx. The BrC and nigrosin optical results by bulk solution absorption are comparable with the properties retrieved from BBCES. The MA200 raw measurements provide reliable absorption Angstrom exponent (AAE) but overestimate kBrC largely. The parameterized overestimates against reference methods depend on light absorption strength, so that the MA200 overestimates more for the less absorbing BrC. The correction factor for MA200 can be expressed well as an exponential function of kBrC or particle single scattering albedo (SSA), and also as a power-law function of the MA200 raw results derived BrC mass absorption efficiency (MAE). The ensemble correction factor regressed for all these BrC and nigrosin is 2.8 based on bulk absorption and 2.7 using BBCES result as reference. Simple radiative forcing (SRF) calculations for different scenarios using the correction for MA200, show consistent SRF when using the aethalometer results after the k(BrC)-dependent correction. (C) 2021 Elsevier B.V. All rights reserved.