Nanoplastics (NPs) and zinc (Zn), both widespread in soil environments, present considerable risks to soil biota. While NPs persist environmentally and act as vectors for heavy metals like Zn, their combined toxicity, especially in soil invertebrates, remains poorly understood. This study evaluates the individual and combined effects of Zn and NPs on earthworm coelomocytes and explores their interactions with Cu/Zn-superoxide dismutase (SOD), an antioxidant enzyme. Molecular docking revealed that NPs bind near the active site of SOD through pi-cation interactions with lysine residues, further stabilized by neighboring hydrophobic amino acids. Viability assays indicated that NPs alone (20 mg/L) had negligible impact (94.54 %, p > 0.05), Zn alone (300 mg/L) reduced viability to 80.02 %, while co-exposure reduced it further to 73.16 %. Elevated levels of reactive oxygen species (ROS) and malondialdehyde (MDA) levels were elevated to 186 % and 173 % under co-exposure, alongside greater antioxidant enzyme disruption, point to synergistic toxicity. Dynamic light scattering and zeta potential (From -13 to -7 mV) analyses revealed larger particle sizes in the combined system, indicative of enhanced protein interactions. Conformational changes in SOD, such as alpha-helix loss and altered fluorescence, further support structural disruption. These findings demonstrate that co-exposure to NPs and Zn intensifies cellular and protein-level toxicity via integrated physical and biochemical mechanisms, providing critical insight into the ecological risks posed by such co-contaminants in soil environments.

Chlorpyrifos contamination is a currently on-going issue with significant environmental impacts. As such, rapid and effective techniques that remove chlorpyrifos from the environment are urgently required. Here, a strain of Pseudomonas nitroreducens W-7 exhibited exceptional degradation ability towards both chlorpyrifos and its major metabolite 3,5,6-trichloro-2-pyridinol (TCP). W-7 can effectively reduce the toxicity of chlorpyrifos and TCP towards a variety of sensitive organisms through its superior degradation capacity. W-7 demonstrated efficient soil bioremediation by removing over 50 % of chlorpyrifos (25 mg/kg) from both sterile and non-sterile soils within 5 days, with significantly reduced half-lives. Additionally, 16S rDNA high-throughput sequencing of the soil revealed that the introduction of W-7 had no significant impact on the soil microbial community. A pivotal hydrolase Oph2876 containing conserved motif (HxHxDH) and a bimetallic catalytic center was identified from W-7. Oph2876 was a heat- and alkali-resistant enzyme with low sequence similarity (< 44 %) with other reported organophosphorus hydrolases, with a better substrate affinity for hydrolysis of chlorpyrifos to TCP. The molecular docking and site-directed mutagenesis studies indicated that the amino acid residues Asp235, His214, and His282, which were associated with the conserved sequence HxHxDH, were crucial for the activity of Oph2876. These findings contribute to a better understanding of the biodegradation mechanism of chlorpyrifos and present useful agents for the development of effective chlorpyrifos bioremediation strategies.

Cadmium (Cd) is a toxic metal primarily found as a by-product of zinc production. Cd was a proven carcinogen, and exposure to this metal has been linked to various adverse health effects, which were first reported in the mid-19th century and thoroughly investigated by the 20th century. The toxicokinetics and dynamics of Cd reveal its propensity for long biological retention and predominant storage in soft tissues. Until the 1950s, Cd pollution was caused by industrial activities, whereas nowadays, the main source is phosphate fertilizers, which strongly contaminate soil and water and affect human health and ecosystems. Cd enters the human body mainly through ingestion and inhalation, with food and tobacco smoke being the primary sources. It accumulates in various organs, particularly the kidney and liver, and is known to cause severe health problems, including renal dysfunction, bone diseases, cardiovascular problems, and many others. On a cellular level, Cd disrupts numerous biological processes, inducing oxidative stress generation and DNA damage. This comprehensive review explores Cd pollution, accumulation, distribution, and biological impacts on bacteria, fungi, edible mushrooms, plants, animals, and humans on a molecular level. Molecular aspects of carcinogenesis, apoptosis, autophagy, specific gene expression, stress protein synthesis, and ROS formation caused by Cd were discussed as well. This paper also summarizes how Cd is removed from contaminated environments and the human body.

Cationic and anionic castor oil-based waterborne polyurethanes (C-WPU/A and C-WPU/C) have great potential for development in agriculture. However, it is still unclear whether these polyurethanes are harmful or toxic to soil fauna. Based on multilevel toxicity endpoints and transcriptomics, we investigated the effects of C-WPU/A and C-WPU/C on earthworms ( Eisenia fetida). ). The acute toxicity results showed that C-WPU/A was highly toxic to the earthworms, whereas C-WPU/C was nearly nontoxic. C-WPU/A significantly affected the body weight, burrowing ability and cocoon production rate of earthworms compared to C-WPU/C. After exposure to C-WPU/ A, the results showed accumulation of reactive oxygen species (ROS), abnormal peroxidase activity, and increased malondialdehyde levels. Additionally, more serious histopathological damage was observed in earthworms, such as epidermal damage, vacuolization, longitudinal muscle disorganization, and shedding of intestinal epidermal cells. At the cellular level, C-WPU/A induced more severe lysosomal damage, DNA damage and apoptosis than C-WPU/A. C-WPU/A made more differentially expressed genes and considerably more enriched pathways at the transcriptional level than C-WPU/C. These pathways are largely involved in cell membrane signaling, detoxification, and apoptosis. These results provide an important reference for elucidating the selective toxicity mechanisms of C-WPU/A and C-WPU/C in earthworms.

Polyethylene microplastics (PE MPs) are the main MPs in agricultural soils and undergo oxidation upon environmental exposure. However, the influence of MP oxidation on phytotoxicity (especially for crop fruit) is still limited. This study aimed to explore the effect of PE MP oxidation on crop toxicity. Herein, a combination of plant phenotyping, metabolomic, and transcriptomic approaches was used to evaluate the effects of lowoxidation PE (LOPE) and high-oxidation PE (HOPE) on wheat growth, grain quality, and related molecular mechanisms using pot experiments. The results showed that HOPE induced a stronger inhibition of wheat growth and reduction in protein content and mineral elements than LOPE. This was accompanied by root ultrastructural damage and downregulation of carbohydrate metabolism, translation, nutrient reservoir activity, and metal ion binding gene expression. Compared with HOPE, LOPE activated a stronger plant defense response by reducing the starch content by 22.87 %, increasing soluble sugar content by 44.93 %, and upregulating antioxidant enzyme genes and crucial metabolic pathways (e.g., starch and sucrose, linoleic acid, and phenylalanine metabolism). The presence of PE MPs in the environment exacerbates crop growth inhibition and fruit quality deterioration, highlighting the need to consider the environmental and food safety implications of MPs in agricultural soils.

Continued application of new chiral fungicide mefentrifluconazole (MFZ) increases its risk to soil ecosystem. However, the toxicity of MFZ enantiomers to soil fauna and whether stereoselectivity exists remains poorly elucidated. Based on multilevel toxicity endpoints and transcriptomics, we investigated the negative effects of racemic, R-(-)-, and S-(+)-MFZ on Eisenia fetida. After exposure to S-(+) configuration at 4 mg/kg for 28 day, its reactive oxygen species levels were elevated by 15.4% compared to R-(-) configuration, inducing enantiospecific oxidative stress and transcriptional aberrations. The S-(+) isomer induced more severe cell membrane damage and apoptosis than the R-(-) isomer, and notably, the selectivity of apoptosis is probably dominated by the mitochondrial pathway. Mechanistically, differential mitochondrial stress lies in: S-(+) isomer specifically upregulated mitochondrial cellular component compared to R-(-) isomer and identified more serious mitochondrial fission. Furthermore, S-(+) conformation down -regulated biological processes associated with ATP synthesis and metabolism, with specific inhibition of mitochondrial respiratory electron transport chain complex I and IV activity resulting in more severe electron flow disturbances. These ultimately mediated enantioselective ontogenetic process disorders, which were supported at phenotypic (weight loss), genetic, and protein (reverse modulate TCTP and Sox2 expression) levels. Our findings offer an important reference for elucidating the enantioselective toxicological mechanism of MFZ in soil fauna.

Pesticides are chemical substances of natural or synthetic origin that are used to eradicate pests and insects. These are indispensable in the agricultural processes for better crop production. Pesticide use aims to promote crop yield and protect the crops from diseases and damage. Pesticides must be handled carefully and disposed of appropriately because they are dangerous to people and other species by default. Environmental pollution occurs when pesticide contamination spreads away from the intended plants. Older pesticides such as lindane and dichlorodiphenyltrichloroethane (DDT) may remain in water and soil for a longer time. These accumulate in various parts of the food chain and cause damage to the ecosystem. Biological techniques in the management of pest control such as importation, augmentation, and conservation, and the accompanying procedures are more efficient, less expensive, and ecologically sound than other ways. This review mainly focuses on the consequences on the targeted and non-targeted organisms including the health and well-being of humans by the use of pesticides and their toxicity. The side effects that occur when a pesticide's LD50 exceeds the accepted limit through oral or skin penetration due to their binding to various receptors such as estrogen receptors, GABA, EGFR, and others. These pesticide classes include carbamates, pyrethroids, organochlorides, organophosphorus, and others. The current study seeks to highlight the urgent requirement for a novel agricultural concept that includes a major reduction in the use of chemical pesticides.

Mesotrione is an emerging environmental contaminant with potential hazards to agricultural environment and nontarget organisms. Microbes are major drivers of mesotrione biodegradation. However, understanding of the degradation characteristics, mechanisms and potential applications of the reported bacteria that can be utilized to eliminate mesotrione is very limited. Here, a novel mesotrione-degrading Klebsiella pasteurii CM -1 with excellent environmental adaptability was isolated, which could completely degrade 100 mg/L mesotrione within 20 h under the optimal condition. Metabolic pathway analysis showed that CM -1 degraded mesotrione to 2-(2hydroxyamino-4-(methylsulfonyl)benzoyl)cyclohexane-1,3-dione, 1,3-dihydro-3-hydroxy-6-(methylsulfonyl) benzo[c]isoxazol-3-yl)cyclohexane-1,3-dione and 2-amino-4-methylsulfonylbenzoic acid. The predicted toxicities of these metabolites were lower than that of mesotrione. Combining genomic analysis and RT-qPCR, nitroreductase-encoding genes nfsA and nfsB were identified as key players driving mesotrione biodegradation. Molecular docking results suggested that residues His215 and Arg218 of NfsA and Lys14, Thr41 and Phe124 of NfsB might be the key sites for their binding to mesotrione. Purified enzyme in vitro assays indicated that NfsA and NfsB could degrade mesotrione under various conditions, and converted it to the same products as CM -1. Some metal cations could significantly enhance the activity of NfsA and NfsB. The Km values of NfsA and NfsB for mesotrione were 0.453 and 1.075 mmol/L. Furthermore, CM -1 could rapidly remove 97.21/96.50% of mesotrione (50 mg/kg) in nonsterilized/sterilized soil within 4 d. This study provided an efficient bioresource with remediation potential for mesotrione residual pollution and new insights into the mechanism of bacterialmediated mesotrione degradation.