Biogrouting has been proposed for improving mechanical properties of soils and rocks, whose performance greatly depends on the location of biocement at pore-scale. To enhance the performance of biogrouting, many strategies were proposed, including the addition of assistants, controlling curing moisture degree, and flocculation of bacteria. Clay is one such assistant which has been proven to be effective, with an assumption of increasing active biocement, i.e. those located between soil particles. In this work, we employed microfluidics to directly observe whether clay minerals can certainly control the location of precipitates and how they function. First of all, the capacity of bentonite and kaolin for adsorbing bacteria were investigated. Then, the location of CaCO3 crystals with and without clay minerals were visually observed using microfluidics. Pore-filling ratios and CaCO3 ratios, which are closely related to permeability and strength of biocemented soils, were quantitatively analyzed from collected images. Finally, the effects of bentonite and kaolin and their dosages on the location of biocement were comprehensively discussed. The results demonstrated that the performance of bentonite and kaolin on adsorbing bacteria and regulating biocement location is distinct due to differences in the morphologies of clays. These findings can help us to improve biogrouting performance on soil stabilization and propose new strategies in various practical applications, such as CO2 sequestration, heavy metal remediation, and oil recovery enhancement, all with a foundational understanding of their mechanisms.

This study presents a method for remediating soils contaminated by organic pollutants through the selective blocking of pores. This technique is based on the use of yield stress fluids, specifically concentrated biopolymer solutions, which, due to their distinctive rheological properties, preferentially flow through high-conductance flow paths. Following the injection of yield stress fluid, its presence redirects subsequent water flow towards the pores that are typically unswept during standard waterflooding. Laboratory experiments at the pore scale were conducted to validate this method and confirm previous findings from core-flooding experiments. Aqueous xanthan gum solutions were used as microscopic blocking agents in well-characterized micromodels exhibiting microscopic heterogeneities in pore size. The impact of polymer concentration, soil wettability and operating conditions (injection pressure and flow rate) on the residual pollutant saturation following treatment was analyzed, enabling the optimization of the remediation strategy. The use of xanthan gum as a blocking agent led to a significant improvement in pollutant removal compared to conventional waterflooding, delivering consistently better results across all cases studied. The method demonstrated strong performance in water-wet medium, with the average polymer concentration yielding the highest efficiency in pollutant removal.