The disposal of tailings in a safe and environmentally friendly manner has always been a challenging issue. The microbially induced carbonate precipitation (MICP) technique is used to stabilise tailings sands. MICP is an innovative soil stabilisation technology. However, its field application in tailings sands is limited due to the poor adaptability of non-native urease-producing bacteria (UPB) in different natural environments. In this study, the ultraviolet (UV) mutagenesis technology was used to improve the performance of indigenous UPB, sourced from a hot and humid area of China. Mechanical property tests and microscopic inspections were conducted to assess the feasibility and the effectiveness of the technology. The roles played by the UV-induced UPB in the processes of nucleation and crystal growth were revealed by scanning electron microscopy imaging. The impacts of elements contained in the tailings sands on the morphology of calcium carbonate crystals were studied with Raman spectroscopy and energy-dispersive X-ray spectroscopy. The precipitation pattern of calcium carbonate and the strength enhancement mechanism of bio-cemented tailings were analysed in detail. The stabilisation method of tailings sands described in this paper provides a new cost-effective approach to mitigating the environmental issues and safety risks associated with the storage of tailings.

Microbially induced carbonate precipitation (MICP) is an eco-friendly technique for weak soil reinforcement. In this study, Sporosacina pasteurii was used to strengthen silty sand after multigradient domestication in an artificial seawater environment. The efficiency of MICP was investigated by carrying out a series of macroscopic and microscopic tests on biocemented silty sand specimens. It was found that the salt ions in seawater impacted bacterial activity. The best activity of the bacterial solution in the seawater environment was achieved after five-gradient domestication, which was approximately 8% lower than that in the deionized water environment. The significant effects of domesticated bacteria on silty sand reinforcement were demonstrated by the content of precipitated carbonate and the unconfined compressive strength (UCS) of the treated specimens. The seawater positively impacted the MICP procedure due to the roles of calcium and magnesium ions, indicated by the X-ray diffraction spectra. The scanning electron microscopy (SEM) results showed that carbonate precipitations distributed primarily on the surfaces and near the contact points of the soil particles, contributing to the soil strength. The cementation solution concentration and injection rate significantly influenced the content and distribution of carbonate precipitations and UCS of the biocemented silty sand, and the values corresponding to good reinforcement efficiency were 1.0 mol/L and 1.0 mL/min, respectively. The results of consolidated undrained triaxial tests showed that the mechanical properties of treated specimens were influenced by biocementation cycles. It was found that the stress-strain behavior of biocemented samples changed from strain hardening to strain softening when the number of reinforcement cycles increased. The peak strength of silty sand was increased by 1.9-3 times after 5 times MICP treatment. The effect of biocementation cycles on the shear strength parameters could be represented by relating the effective friction angle and effective cohesion of biocemented silty sand to the carbonate content.

Soil Pb contamination is inevitable, as a result of phosphate mining. It is essential to explore more effective Pb remediation approaches in phosphate mining wasteland soil to ensure their viability for a gradual return of soil quality for cultivation. In this study, a Pb-resistant urease-producing bacterium, Serratia marcescens W1Z1, was screened for remediation using microbially induced carbonate precipitation (MICP). Magnesium polypeptide (MP) was prepared from soybean meal residue, and the combined remediation of Pb contamination with MP and MICP in phosphate mining wasteland soil was studied. Remediation of Pb using a combination of MP with MICP strain W1Z1 (WM treatment) was the most effective, with the least exchangeable Pb at 30.37% and the most carbonate-bound Pb at 40.82%, compared to the other treatments, with a pH increase of 8.38. According to the community analysis, MP moderated the damage to microbial abundance and diversity caused by MICP. Total nitrogen (TN) was positively correlated with Firmicutes, pH, and carbonate-bound Pb. Serratia inoculated with strain W1Z1 were positively correlated with bacteria belonging to the Firmicutes phylum and negatively correlated with bacteria belonging to Proteobacteria. The available phosphate (AP) in the phosphate mining wasteland soil could encapsulate the precipitated Pb by ion exchange with carbonate, making it more stable. Combined MPMICP remediation of Pb contamination in phosphate mining wasteland soil was effective and improved the soil microenvironment.

Microbially induced carbonate precipitation (MICP) represents a technique for biocementation, altering the hydraulic and mechanical properties of porous materials using bacterial and cementation solutions. The efficacy of MICP depends on various biochemical and environmental elements, requiring careful consideration to achieve optimal designs for specific purposes. This study evaluates the efficiency of different MICP protocols under varying environmental conditions, employing two bacterial strains: S. pasteurii and S. aquimarina, to optimize soil strength enhancement. In addition, microscale properties of carbonate crystals were investigated and their effects on soil strength enhancement were analyzed. Results demonstrate that among the factors investigated, bacterial strain and concentration of cementation solution significantly influence the biochemical aspect, while temperature predominantly affects the environmental aspect. During the MICP treatment process, the efficiency of chemical conversion through S. pasteurii varied between approximately 80% and 40%, while for S. aquimarina, it was only around 20%. Consequently, the CaCO3 content resulting from MICP treatment using S. pasteurii was significantly higher, ranging between 5% and 7%, compared to that achieved with S. aquimarina, which was about 0.5% to 1.5%. The concentration of the cementation solution also plays a pivotal role, with an optimized value of 0.5 M being critical for achieving maximum efficiency and CaCO3 content. The ideal temperature span for MICP operation falls between 20 degrees C and 35 degrees C, with salinity and oxygen levels exerting minor impact. Furthermore, although salinity influences the characteristics of formed carbonate crystals, its effect on unconfined compressive strength (UCS) values of MICP-treated soil remains marginal. Samples subjected to a one-phase treatment, adjusted to pH values between 6.0 and 7.5, exhibit roughly half the UCS strength compared to the two-phase treatment. These findings hold promising potential for MICP applications in both terrestrial and marine environments for strength enhancement.

This study proposed an improved bio-carbonation of reactive magnesia cement (RMC) method for dredged sludge stabilization using the urea pre-hydrolysis strategy. Based on unconfined compression strength (UCS), pickling-drainage, and scanning electron microscopy (SEM) tests, the effects of prehydrolysis duration (T), urease activity (UA) and curing age (CA) on the mechanical properties and microstructural characteristics of bio-carbonized samples were systematically investigated and analyzed. The results demonstrated that the proposed method could significantly enhance urea hydrolysis and RMC bio-carbonation to achieve efficient stabilization of dredged sludge with 80% high water content. A significant strength increment of up to about 1063.36 kPa was obtained for the bio-carbonized samples after just 7 d of curing, which was 2.64 times higher than that of the 28-day cured ordinary Portland cement-reinforced samples. Both elevated T and UA could notably increase urea utilization ratio and carbonate ion yield, but the resulting surge in supersaturation also affected the precipitation patterns of hydrated magnesia carbonates (HMCs), which weakened the cementation effect of HMCs on soil particles and further inhibited strength enhancement of bio-carbonized samples. The optimum formula was determined to be the case of T = 24 h and UA = 10 U/mL for dredged sludge stabilization. A 7-day CA was enough for bio-carbonized samples to obtain stable strength, albeit slightly affected by UA. The benefits of high efficiency and water stability presented the potential of this method in achieving dredged sludge stabilization and resource utilization. This investigation provides informative ideas and valuable insights on implementing advanced bio-geotechnical techniques to achieve efficient stabilization of soft soil, such as dredged sludge. (c) 2024 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/ licenses/by-nc-nd/4.0/).

Soil biocementation through microbially induced carbonate precipitation (MICP) is a promising technique for improving soil behavior in a nondisruptive manner, particularly for rehabilitation and retrofitting applications. Previous studies characterizing the shear behavior of biocemented soils have concentrated on poorly graded sands, whereas research on well-graded gravelly soils, which are extensively used in shallow geotechnical structures, has been lacking. Mohr-Coulomb strength parameters have been predominately employed to interpret the macromechanical effects of biocementation, but the previously reported findings show significant contradictions. In this study, a well-graded aggregate, representative of commonly used well-graded gravelly soils, was biocemented and subjected to monotonic drained triaxial compression. The test results show remarkable improvements in shear behavior, with the observed changes in stress-strain responses, strength and stiffness development, and stress dilatancy agreeing with those reported for biocemented sands as well as conventional cemented soils. Relatively low cementation levels can effectively rectify the mechanical performance caused by poor compaction to that seen at optimal levels, demonstrating the feasibility and potential of biocementation for improving soils of this type. Detailed analysis of the results reveals the decisive role of cementing bonds and their degradation in causing behavioral changes at different shearing stages. The theories of bonded structure and force-chain evolution are used to explain the preyielding observations, while an analytical approach capable of quantifying the evolution of different strength components is presented for postyielding macromechanical characterization. Conversely to the inference drawn from the strength parameters, the largest improvement is found in the frictional rather than the dilative and cohesive components of strength. Further analysis reveals the commonality of the macromechanical effects of biocementation, density, and confinement, and a unique relationship between macromechanical composition and peak stress ratio emerges.