Microbially induced calcium carbonate precipitation (MICP) is an emerging ecofriendly microbial engineering technique that utilizes urease-producing microorganisms to enhance the mechanical properties of soils. Sporosarcina pasteurii (S. pasteurii) stands out among these microorganisms as an efficient urease producer. However, field trials often lead to less-than-optimal experimental outcomes due to the presence of native soil microbes. To evaluate the impact of indigenous microorganisms on the effectiveness of MICP at the site, bacteria isolated from natural soil, classified of on-site low-ureolysis and high-ureolysis bacteria (OSLUB and OSHUB, respectively), were combined with S. pasteurii to conduct MICP experiments both in microfluidic chips and sand columns. Analysis covered the bacterial population, urease activity, pH changes, calcium carbonate crystal count and volume, as well as the unconfined compressive strength (UCS) of reinforced samples. Experimental results revealed that combining OSLUB with S. pasteurii led to a reduction in bacterial activity of 74% to 84% by 120 h, resulting in an approximately 60% decrease in the chemical conversion rate and the UCS of MICP-treated soils was 60% lower than the S. pasteurii. However, when OSHUB is mixed with S. pasteurii, although there is a reduction in bacterial activity by 49% to 54% by the 120-h mark, the decrease remains less pronounced than the activity decrease observed in S. pasteurii alone, which is 64%. Consequently, the rates of calcium carbonate chemical conversion were enhanced by 9% to 45%, and the UCS of the reinforced sand columns showed a slight improvement relative to the control group. This research highlights the distinct impacts of OSLUB and OSHUB on the efficiency of MICP on location. The main difference between OSLUB and OSHUB lies in their respective effects on pH levels following mixing. OSLUB tends to decrease the pH level gradually in the combined bacterial environment, while OSHUB, in contrast, increases the pH level over time in the same setting. The maintenance of both high bacterial activity and high precipitation rates is crucially dependent on pH levels, highlighting the importance of these findings for enhancing MICP efficiency in field applications. Strategies that either diminish the presence of OSLUB while augmenting that of OSHUB, or that sustain a relatively high pH level, could be valuable. These avenues promise significant improvements and merit further investigation in future studies.

Soil erosion is a common phenomenon which causes lots of geological and engineering disasters. Clayey soil erosion control is a hot research topic and a challenging issue for its low permeability and lack of effective infiltration of treatment solutions. In this study, a coupling microbially induced calcium carbonate precipitation (MICP)-sand column method was proposed as a promising and sustainable technique to mitigate surface clayey soil erosion with adjustable treatment depth. Seven groups of soil samples were prepared, including pure soil, MICP-treated soil, and MICP-sand column method-treated soil. A series of disintegration tests and penetration tests were conducted to investigate the method feasibility and adjusting mechanism of erosion mitigation with varying sand column heights and diameters. Compared to pure soil sample, sample treated by MICP-sand column (6 cm-height and 3 cm-diameter) method could reach a maximum reduction of 50 % in ultimate disintegration rate and a 30 % increase in the highest penetration resistance. The sample has a 7.9 cm effective treatment depth, which is 5.3 times of the MICP-treated sample. The mechanism of erosion mitigation can be attributed to that the sand column serves as a favorable path for the bacteria suspension and cementation solution in low-permeabilityclayey soil and improves the effective depth of MICP treatment. Adjusting the height and diameter of sand column can change the calcium carbonate distribution, especially at the locations near the surface and around the sand column. In this study, the optimal height and diameter are 6 cm and 3 cm, respectively, and finally form a U-shape three-layer structure. The structure significantly increases the proportion of the hard crust layer and weak-cemented layer with high hydro-mechanical properties. In conclusion, the coupling MICP-sand column method with reasonable height and diameter of sand column shows the ability to control surface erosion of soil and has better long-term mitigation performance.