Green soybean (edamame), an early-harvested soybean, is a popular vegetable in Asia and is recognised as a healthy vegetable in the other parts of the world. In Japan, edamame yield has gradually decreased over the last 30 years, despite similar cultivation areas. Damage caused by the soybean cyst nematode (SCN), Heterodera glycines, is one of the main causes. We surveyed the distribution of SCN in different locations and found a wide distribution of SCN across Japan. Different control measures are available, such as chemical control using fumigants or a granular type of nematicide, solarisation, and rotation with non-host crops. We are developing a new type of biological control method, which comprises short-term field cultivation and soil incorporation of mung bean. This method not only decreases the SCN density in soil but also mitigates soil erosion and nitrate leaching. For future SCN control it is essential to establish an environmentally friendly management strategy.

The worldwide contamination of waters and food by herbicides is a major health issue, yet the toxic effects of herbicides to non-target organisms and ecosystems have been poorly summarized. Here we review the effects of herbicides belonging to the groups of chloroacetanilides, imidazolinones, sulfonylureas, and pyrimidinylcarboxylic, on small invertebrates, high vertebrates, plants, and the surrounding ecosystems. We describe toxicity in terms of behavioural changes, molecular biosynthesis, endocrine disruption, immunological responses, enzymatic alteration, and reproductive disorders. Strategies to decrease toxic effects are also presented. We observe widespread toxicity threats in amphibians and major aquatic species. Each herbicide group displays a different toxicity risk. For instance, chloroacetanilides display higher risks to soil, aquatic, algal, cyanobacteria, and terrestrial species, whereas alachlor, acetochlor, and metolachlor are highly carcinogenic to humans. Most imidazolinone herbicides cause phytotoxicity in non-target and succeeding crops. Sulfonyl-urea herbicides are severely toxic to soil microbes and succeeding crops. Pyrimidinylcarboxy herbicides are more toxic to soil microbes, aquatic species, and rats.

The Kugino wind farm at Japan was seriously damaged in the severe Kumamoto earthquake, characterizing as all three pile group cracks but only one tower buckling. This study aims to reveal the failure mechanism underlying such damage pattern through the Beam on Nonlinear Winkler Foundation (BNWF) analyses, where the soilfooting interaction is considered with a new q-z model (QzSimple6). It identifies three parameters in a hyperbolic function to match any desired modulus reduction curve, whereas adjusts the unloading-reloading curves iteratively with the Ishihara-Yoshida rule to achieve site-specific soil damping curve. The QzSimple6-based BNWF analyses quantitatively reproduces centrifuge test results of a pile group foundation system, and newly reveals the soil-footing interaction does not influence pile bending moments but reduces the point mass acceleration. A parametric study is conducted on the full BNWF model with identifying pile group supported wind turbine, but with scaling soil stiffness and strength. The thrust force is attracted from the aero-elastic analysis in OpenFAST and the free-filed seismic displacement are calculated with the site response analysis in OpenSees. The simulation shows consistency with site observations that the No.2 wind turbine tower is destined to buckling at the height of around 13.9 m due to the sudden reduction of tower thickness, while No.1 and No.3 towers could remain safe potentially because soil properties under them are softer than that under the No.2 tower. In contrast, all three pile groups are found to be cracked under the Kumamoto earthquake intensity since the pile bending moment relies on the footing rigidity rather than the footing-soil interaction.

BACKGROUNDA major impact of invasive Myocastor coypus in their introduction range is the collapse of riverbanks and nearby infrastructure, such as railway lines, due to the species' burrowing activities. Because widespread implementation of preventive measures along watercourses is unfeasible, identifying susceptible areas is key to guide targeted management actions. This study used species-habitat models to: (i) identify local environmental features of the railway line/watercourse intersections (RLWIs) that make them particularly susceptible to coypu damage, and (ii) predict species occurrence probability over a wide lowland-hilly area of northern Italy (Lombardy) to identify priority areas for monitoring. RESULTSLocal-scale models identified that the RLWIs most susceptible to burrowing were those surrounded by arable land with interspersed hedgerows locally characterized by high herbaceous vegetation and clay soil. In urbanized areas and areas of intensive agriculture, coypu dens were generally located significantly closer to the railway, increasing the risk of collapse. A landscape-scale species distribution model showed that lowland areas along major rivers and lake shores, and also agricultural areas with a dense minor hydrographic network, particularly in the southeast of the study area, are more likely to be occupied by coypu. CONCLUSIONLocal-scale models showed that specific environmental characteristics increase the risk of burrowing near RLWIs. The landscape-scale model allowed us to predict which areas require thorough monitoring of RLWIs to search for such local characteristics to implement preventive management measures. The proposed model-based framework can be applied to any geographical context to predict and prevent coypu damage. (c) 2024 Society of Chemical Industry.

The incident of Chernobyl Nuclear Power Plant (CNPP) explosion has pioneered a plethora of studies unfolding various biological effects of radiation stress on several living systems. Determining radiation dose rates at which both acute and chronic biological effects occur in different biological systems will aid in the ex-situ generation of radiation-tolerant organisms. So far, the accumulation of data on different radiation doses from Chernobyl area demonstrating various biological impacts has not been documented altogether vastly. Therefore, this review aims to document the recorded doses in CNPP over the years at which different biological changes have been observed in plants, soil, aquatic organisms, birds, and animals. A total of 72 peer-reviewed papers obtained from PubMed, Google Scholar, Scopus, and Research4life were included in this review. A few factors have come under attention in this review. Firstly, plant and soil systems combinedly showed the most published studies after the catastrophe where plants showed a higher frequency of DNA methylation in their genome to resist radiation stress. Secondly, reduced species abundance, chromosomal aberrations, increased sterility, and mortality were mostly observed in the aftermath of Chernobyl catastrophe among plants, soil, aquatic organisms, birds, and small mammals. Furthermore, major scares of data after 2018 were prominently observed. Very few studies on radiation dose levels after 2018 are available. Hence, a major research area has emerged for radiation biologists to study present radiation levels and any genetic changes in the recent generation of the original victim species. This will help provide a standard dataset that can act as a reference resource for radiation biologists and future research on the impact of both acute and chronic radiation on the different biological systems. [GRAPHICS] .

The 2017 Pohang earthquake, with a moment magnitude (M) of 5.5, caused severe building damage and widespread liquefaction. In this study, we evaluate the applicability of ground response and liquefaction triggering analyses for the Pohang earthquake using deep shear wave velocity (VS) profiles. The VS profiles are obtained at Handong University and the Songdo Pine Forest by inverting the Rayleigh wave dispersion curves based on microtremor array measurements (MAM) and multi-channel analysis of surface waves (MASW). In onedimensional effective stress analyses for the two sites, we consider the uncertainty of the nonlinear soil properties for three cases and use 118 rock outcrop motions. At Handong University, the spectral accelerations of surface ground motions are larger than those of the current Korean design spectra with a return period of 500 years at the natural period of the damaged buildings. At the Songdo Pine Forest, for the Case 2, numerous ground motions result in the maximum pore water pressure ratio of 1 (i.e., liquefaction occurrence). Furthermore, we calculate the liquefaction potential index (LPI) values using the VS-based simplified method. To compute the cyclic stress ratio for depths, we utilize the peak ground accelerations estimated by ground response analyses and estimated by stress reduction factor (rd), respectively. The LPI values, based on the ground response analyses, range from 0 to 4, indicating minor or no damage, while the LPI value using the rd is zero. The results of the ground response and liquefaction triggering analyses are similar to the actual damage cases.

Exposure to xenobiotics can increase the production of reactive oxygen species (ROS). When detoxification organs such as the intestines and liver cannot neutralise these xenobiotics, it can induce oxidative stress and cause damage to tissues. Therefore, cell-based bioassays that indicate intracellular ROS production are a useful screening tool to evaluate the effect of these chemicals. Although flow cytometry is commonly used to measure ROS in cells, many research laboratories in the Global South do not always have access to such specialised instrumentation. Therefore, we describe a sensitive but low-cost method that can easily be used to determine ROS production in vitro. This method employs the fluorogenic dye, 2 ' ,7 ' -dichlorodihydrofluorescein diacetate (H2DCF-DA), which emits fluorescence after being oxidised to a fluorescent derivative. Since the H2DCF-DA bioassay indicates non-specific ROS production it can be used as a marker of overall oxidative stress. This method was validated by exposing human duodenum epithelial adenocarcinoma (HuTu-80) and rat liver epithelial hepatoma (H4IIE-luc) cells to agricultural soil samples. center dot Production of ROS can be determined in vitro in intestinal and liver cells. center dot This method is inexpensive and can be easily performed in standard laboratories. center dot The method provides a tool for the high-throughput screening of environmental samples.

Background Alpine ecosystem underlain by permafrost is considered as one of the most vulnerable ecosystems to disturbance, especially the alpine grassland on the Tibetan plateau with an altitude above 4000 m. Plateau pika (Ochotona curzoniae) burrowing can create distinctive bare grounds and cause micro-topographical heterogeneity in alpine grasslands. The burrowing-induced changes in microtopography may directly alter plant and soil interactions as well as ecosystem carbon cycle, which have rarely been studied in Tibetan alpine grasslands. Methods To test the responses of ecosystem respiration (Re) to pika burrowing-induced changes in microtopography, we investigated plant characteristics, soil properties and Re from the bare grounds and vegetated grounds in the alpine meadow and steppe on the Tibetan Plateau. Results Our study showed that vegetation cover, species richness, plant biomass, soil moisture (SM), soil organic carbon (SOC), total nitrogen (STN), soil microbial biomass carbon (MBC) and nitrogen (MBN) in the bare grounds were significantly lower than in the vegetated grounds in both alpine meadow and alpine steppe (P < 0.05). However, soil temperature and inorganic nitrogen tended to increase in the bare grounds. The growing season Re was significantly lower in the bare grounds than that in the vegetated grounds (P < 0.01). Pika burrowing had negative effects on Re and its temperature sensitivity in both alpine vegetations (P < 0.05). The relative changes in Re due to burrowing-induced changes in microtopography were positively correlated with the burrowing caused changes of AGB, BGB, SOC and MBC (P < 0.05). Pika burrowing-induced changes in soil temperature, soil moisture, plant biomass and microbial biomass are the major factors for the decrease of Re in the bare grounds. Conclusion In view of the large number of pika burrows in the alpine grasslands and the loss of soil organic carbon due to pika bioturbation, the impacts of pika burrowing-induced changes in microtopography on Re must be considered in predicting the carbon cycle in alpine grasslands.

Alaska's North Slope is especially vulnerable to climatic change because higher latitudes are subject to positive snow- and sea ice-atmosphere feedbacks under warming conditions and because the dynamics of frozen seascapes and landscapes are tightly determined by thermal regime. Shifts in timing and magnitude of freeze-thaw processes are observed to have or expected to have non-linear, threshold-crossing impacts on sea ice, landforms, and biota. Observed changes in North Slope surface air temperatures and precipitation were non-monotonic over the last century, but have trended upward for the last several decades. These changes are linked to hemispheric climate dynamics, reflected in North Pacific and Arctic Oscillation circulation indices. Projected anthropogenic climate changes-with the possibility of continued warming, increased storm frequency and intensity, and decreased insulating snow cover-portend an uncertain future for this domain. Current or foreseen physical system shifts include: (1) declining seasonal and permanent sea ice extent and character, (2) rapid coastal erosion due to storm exposure over a longer near-shore ice-free season, (3) deeper soil active layer over warmer permafrost, along with altered thermokarst processes-contributing to thaw lake expansion, surface drainage re-organization, and hillslope instability. Biogeophysical responses encompass (1) modified surface-atmosphere energy balance from snow cover, vegetation, and hydrologic change and (2) shifted soil and wetland biogeochemical dynamics, including accelerated carbon efflux. Climate-driven plant community shifts on the North Slope result from the interplay of climate, vegetation response, and landscape processes. Some transitions involve stabilizing, others destabilizing plant-permafrost feedbacks. Impacts on caribou, migratory avifauna, and freshwater biota are through direct effects of climate on organism physiology and reproductive biology and indirectly through disruption of habitat mosaics (including along migratory routes) and shifts in competition and trophic linkages. The North Slope's physical and biological vulnerabilities to shifting climate and observed leading indicators of change are compelling reasons for land managers to consider climatic instability as a threat in conjunction with other known stressors while seeking strategies for protection of this domain's natural heritage and ecosystem services.

[1] Large variations in the composition, structure, and function of Arctic ecosystems are determined by climatic gradients, especially of growing-season warmth, soil moisture, and snow cover. A unified circumpolar classification recognizing five types of tundra was developed. The geographic distributions of vegetation types north of 55degreesN, including the position of the forest limit and the distributions of the tundra types, could be predicted from climatology using a small set of plant functional types embedded in the biogeochemistry-biogeography model BIOME4. Several palaeoclimate simulations for the last glacial maximum (LGM) and mid-Holocene were used to explore the possibility of simulating past vegetation patterns, which are independently known based on pollen data. The broad outlines of observed changes in vegetation were captured. LGM simulations showed the major reduction of forest, the great extension of graminoid and forb tundra, and the restriction of low- and high-shrub tundra (although not all models produced sufficiently dry conditions to mimic the full observed change). Mid-Holocene simulations reproduced the contrast between northward forest extension in western and central Siberia and stability of the forest limit in Beringia. Projection of the effect of a continued exponential increase in atmospheric CO2 concentration, based on a transient ocean-atmosphere simulation including sulfate aerosol effects, suggests a potential for larger changes in Arctic ecosystems during the 21st century than have occurred between mid-Holocene and present. Simulated physiological effects of the CO2 increase (to >700 ppm) at high latitudes were slight compared with the effects of the change in climate.