The importance of green areas in today's modern city concept is increasing day by day. In this understanding, the use of turfgrass [e.g. Bentgrass (Agrostis spp. L.); Kentucky Bluegrass (Poa pratensis L.); Common Bermudagrass Cynodon dactylon (L.) Pers. (Poales: Poaceae)] in sports fields is getting important. Golf courses mainly occurs turfgrass and not much nematological studies has been done in courses of T & uuml;rkiye. In this study, total of 51 soil and 3 water samples were taken from golf courses in Antalya, T & uuml;rkiye's largest golf tourism destination, in 2021. Within the scope of this study, plant parasitic nematode (PPN) species belonging to the genera Aphelenchoides Fischer, 1894 (Tylenchida: Aphelenchoididae), Aphelenchus Bastian, 1865 (Tylenchida: Aphelenchoididae), Criconemella (De Grisse & Loof, 1965) (Tylenchida: Criconematidae), Ditylenchus Filipjev, 1936 (Tylenchida: Anguinidae), Helicotylenchus Steiner, 1945 (Tylenchida: Hoplolaimidae), Hemicriconemoides Chitwood & Birchfield, 1957 (Tylenchida: Criconematidae), Hemicycliophora de Man, 1921 (Tylenchida: Hemicycliophoridae), Hoplolaimus von Daday, 1905 (Tylenchida: Hoplolaimidae), Longidorus Micoletzky, 1922 (Dorylaimida: Longidoridae), Paratrichodorus Siddiqi, 1974 (Triplonchida: Trichodoridae) and Tylenchus Bastian, 1865 (Tylenchida: Tylenchidae) were identified using morphological and morphometric methods. The most detected species in the samples was Hemicycliophora punensis Darekar & Khan, 1980 (Rhabditida: Hemicycliophoridae) (22.22%), while the least detected PPN species was Helicotylenchus dihystera (Cobb, 1893) Sher, 1961 (Tylenchida: Hoplolaimidae) (3.70%). In this study, it is important there are virus vector species among the identified plant parasitic nematode genera. These nematode species can play an active role in the spread of various viral diseases in turfgrass areas. In turfgrass areas where very sensitive cultivation is carried out, such as golf courses, PPN's cause direct damages by feeding, which serve as the source of entry of pathogens into the plants. This situation increases the prevalence and severity of the disease in infected fields. Therefore, early detection of the presence of PPN's in cultivation areas is important to determine effective control strategies.
Root-knot nematodes were discovered in severely declining creeping bentgrass putting greens at a golf course in Indian Wells, Riverside County, California. The exhibited disease symptoms included chlorosis, stunted growth, and dieback. Based on morphological examination and measurements of J2 females and males, it was suggested that the causal pathogen was Meloidogyne marylandi. This identification was confirmed by analysis of the D2-D3 expansion segments of 28S rRNA and COI gene sequences. The host status of 28 plant species was evaluated in greenhouse trials. All tested monocots, except rye and Allium species, were found to be hosts, while no reproduction occurred on dicots. Temperature-tank experiments helped determine that the life cycle of M. marylandi was completed between 17-35 degrees C, with a base temperature of 8.3 degrees C and a required heat sum of 493 degree-days (DD). In greenhouse trials in pasteurized soil and near-ideal growing conditions, M. marylandi did not cause significant growth reduction of creeping bentgrass cv. Penn A-4, even at very high J2 inoculation densities. It is highly probable that other biotic and abiotic factors contributed to the observed putting green damage.