Permafrost thaw has the potential to release ancient particulate and dissolved organic matter that had been stored for thousands of years. Previous studies have shown that dissolved organic matter from permafrost is very labile and can be used by heterotrophic microbes close to the thaw area. However, it is unknown if ancient particulate organic matter can also be utilized. This study aims to investigate whether arctic microbial communities (bacteria and Archaea) incorporate ancient organic matter potentially released from thawing permafrost into their biomass. We compare and contrast the radiocarbon signatures of microbial lipids and higher plant biomarkers (representing terrestrial organic matter) from five soil profiles and seven deltaic lake sediment cores from the Mackenzie River drainage basin, Arctic Canada. In the surface soils, modern to post-modern short-chain fatty acids (SCFA) ages indicate in situ microbial production, with differential rates of organic carbon (OC) cycling depending on soil moisture. In contrast, SCFA in deeper soils display millennial ages, which likely represent the microbial necromass preserved through mineral association. In deltaic lakes that are disconnected from the river, generally old SCFA suggests the uptake of pre-aged OC by bacteria. In perennially connected lakes, pre-aged SCFA could originate from in situ microbial uptake of old OC or from the Mackenzie River. Higher plant-derived long-chain fatty acids (LCFA) present older radiocarbon ages, reflecting mineral stabilization during either pre-aging in soils (for high closure lakes) or riverine transport (for no and low closure lakes). Archaeal lipids are younger than SCFA and LCFA in high closure lakes, and older in low and no closure lakes, mirroring bulk radiocarbon signatures due to their heterotrophic production. These radiocarbon signatures of bacterial biomarker lipids may therefore reflect microbial incorporation of ancient OC (e.g., derived from permafrost thaw) or exceptional preservation (e.g., through mineral stabilization). Hence, even in relatively high OC environments such as arctic aquatic ecosystems, microbes can rely on ancient OC for their growth.

Organic carbon stored in high-latitude permafrost represents a potential positive feedback to climate warming as well as a valuable store of paleoenvironmental information. The below-freezing conditions have effectively removed permafrost organic material from the modern carbon cycle and preserved its pre-freezing bulk and molecular states. The conditions that lead to efficient burial of organic carbon (OC) within permafrost were investigated by measuring OC stocks, past accumulation rates, and biogeochemical composition of a permafrost core taken from Interior Alaska dating back to 40 ka. The post-glacial Marine Isotope Stage 1 is represented by the top 1.2 m of the core and contains 64.7 kg OC/m(2) with an accumulation rate of 4.3 g OC/m(2)/yr. The sediments that accumulated around the Last Glacial Maximum contain 9.9 kg OC/m(2) with an accumulation rate of 0.5 g OC/m(2)/yr. Carbon storage (144.7 kg OC/m(2)) and accumulation (26.1 g OC/m(2)/yr) are both observed to be greatest between 35 and 40 ka, late during the Marine Isotope Stage 3 global interstadial. The extent of OC degradation was assessed using lignin and amino acid biomarkers with both approaches indicating well-preserved contemporary active layer and interstadial OC, whereas stadial OC was highly degraded. Lignin compositional indices throughout the core appear altered by sorptive processes that confounded some expected trends in the overall organic matter composition, while amino acids provided a more integrated pattern of change. Significant correlations between carbon-normalized hydroxyproline and total lignin concentrations further support the usefulness of hydroxyproline as an indicator for the abundance of plant organic matter. A novel amino acid plant-microbial index of the ratio of microbial-specific muramic acid and diaminopimelic acid biomarkers to the plant-specific hydroxyproline biomarker, indicate a transition from plant-dominated organic matter in fresh organic soils (index values of 0.01-0.20) to more microbial-dominated organic matter in degraded mineral soils (index values of 0.50-2.50). The branched glycerol dialkyl glycerol tetraether composition is complex and is not immediately compatible with existing temperature transfer functions. Residence time within the active layer is interpreted to integrate key factors such as primary productivity, inorganic sediment delivery, and other climate factors that control soil organic matter degradation. The Marine Isotope Stage 3, mid-Wisconsin interstadial period at this locality was forest-dominated and suggests the currently prevailing tundra ecotone is sensitive to environmental change. The majority of buried permafrost OC is high in degradability and if thawed, would be expected to be highly vulnerable to microbial decomposition. (C) 2019 Elsevier Ltd. All rights reserved.