Phaseolus vulgaris is one of the most widely consumed legumes across the globe. It is rich in protein, carbohydrates, fiber, bioactive compounds, and mineral contents. Occurrences of drought under changing climate have posed a challenge to the quality and quantity of the crop yield. Our study, taking Phaseolus vulgaris as a test legume, reports that as a soil amendment, biochar and farmyard manure mitigated drought-induced damage in the plant. The experiment was set up in a greenhouse with 1 m(2) blocks together arranged in factorial randomized block design. Water withdrawal was done for 15 days in either vegetative or reproductive stage of the crop to simulate drought conditions. Biochar or Farmyard manure was applied at the rate of 10t ha(-1) as soil amendment and each treatment was replicated thrice. Higher photosynthesis (up to 23% and 16%, respectively), leaf protein (41% and 30%, respectively), grain protein (4% and 19%, correspondingly), and in-vitro protein digestibility (60% and 254%, respectively) were noted under application of both the amendments. Grain quality in terms of protein content, in-vitro protein digestibility, and protein fractions of Phaseolus vulgaris was improved under drought due to the addition of biochar and farmyard manure. However, the application of those amendments at the rate of 10t ha(-1), especially farmyard manure, had a negative impact on the antinutritional factor (phytic acid) and mineral bioavailability. Between the two amendments studied, farmyard manure proved to be more efficient in promoting the overall grain quality of P. vulgaris, but its inimical effects must be concorded.
Copper (Cu) stress is a serious problem in contaminated soils that causes significant reduction in okra growth and production. To determine the toxic effect of Cu on okra plant and identify an effective way to mitigate Cu toxicity on okra, seeds of okra were inoculated with Bacillus subtilis and sown with farmyard manure. There were 13 treatments (T-0= Control, T-1= 400 mg kg(-1) of soil Cu, T-2= 400 mg kg(-1) of soil Cu+ B. subtilis, T-3= 400 mg kg(-1) of soil Cu+ FYM, T-4= 400 mg kg(-1) of soil Cu+ B. subtilis+ FYM, T-5= 450 mg kg(-1) of soil Cu, T-6= 450 mg kg(-1) of soil Cu+ B. subtilis, T-7=450 mg kg(-1) of soil Cu+ FYM, T-8=450 mg kg(-1) of soil Cu+ B. subtilis+ FYM, T-9=500 mg kg(-1) of soil Cu, T-10=500 mg kg(-1) of soil Cu+ B. subtilis, T-11=500 mg kg(-1) of soil Cu+ FYM, T-12=500 mg kg(-1) of soil Cu+ B. subtilis+ FYM) planned with the complete randomize design (CRD). Results of this research reveal that the okra production and soil physiological properties decreased with the addition of Cu in the soil, this is a new approach for sustainable crop production under Cu stress condition. 500 mg kg(-1) of Cu in the soil have the more negative effect on plant growth but negative effect of Cu can be control with the addition of B. subtilis and FYM. Combine application of B. subtilis and FYM improve the soil properties and plant growth by improving the microbial activities, nutrients availability in the soil and production of growth hormones.