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The geotechnical engineering properties of seabed soils hold vital significance in various marine engineering domains, including offshore petroleum platforms, underwater tunnels, oil and gas pipelines, submarine cables, topographic surveys, and underwater disaster forecasting. However, existing equipment for measuring seabed geotechnical parameters generally suffers from issues such as the inability to achieve in-situ real-time measurements, limited measurement parameters, cumbersome operational procedures for engineering measurements, and low measurement accuracy. In response to these existing problems, this paper proposes a selfcontained static cone penetration probe and measurement control system suitable for multi-parameter measurements in deep-sea environments. The system has established a three-in-one measurement approach for cone tip resistance, sidewall frictional force, and pore water pressure, based on a self-contained structure with temperature and attitude information as calibration references. For the weak strain signals, signal conditioning, acquisition, and storage circuits were designed, and their performance parameters were validated in conjunction with simulation software to ensure their rationality. On the basis of hardware circuits, multitasking scheduling was achieved using the FreeRTOS real-time operating system embedded in the chip. This enabled data collection, storage, monitoring, and low-power design, thus realizing the functionality of multi-parameter, rapid-response data acquisition and storage.

期刊论文 2024-08-01 DOI: 10.1016/j.oceaneng.2024.117742 ISSN: 0029-8018

Tobacco bacterial wilt (TBW) caused by Ralstonia solanacearum is a serious soil -borne disease, which seriously damages the growth of tobacco crops. Bacillus velezensis A5 was isolated from 3000 m deep-sea sediments of the Pacific Ocean, and was found to be antagonistic to TBW. Here, we report the complete genome sequence of strain A5, which has a 4,000,699 -bp single circular chromosome with 3827 genes and a G + C content of 46.44%, 87 tRNAs, and 27 rRNAs. A total of 12 gene clusters were identified in the genome of strain A5, which were responsible for the biosynthesis of antibacterial compounds, including surfactin, bacillaene, fengycin, difficidin, bacillibactin, and bacilysin. Additionally, strain A5 was found to contain a series of genes related to the biosynthesis of carbohydrate -active enzymes and secreted proteins. Our results indicate that strain A5 can be considered a promising biocontrol agent against TBW in agricultural fields.

期刊论文 2024-02-01 DOI: 10.1016/j.margen.2024.101087 ISSN: 1874-7787
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