To address the low utilization rate of construction waste soil and the environmental impact of traditional cement solidification, this study investigates the effect of desulfurized gypsum and silica fume in synergy with cement for construction waste soil. The effects of solidifying material dosage, liquid-to-solid ratio, and mixing ratio on mechanical properties were analyzed. Optimal performance was achieved with the dosage of solidifying material was 20%, the liquid-to-solid ratio was 0.2, and the mixing ratio of desulfurized gypsum, silica fume, and cement was 2:1:1, meeting the requirements of the technical specification for application of road solidified soil (T/CECS 737-2020). This formulation is referred to as FS-C type solidified soil. A self-fabricated carbonation device was employed to assess carbonation methods, time, and curing age on the mechanical properties of solidified soil. Carbonation for 6 h post-molding significantly enhanced strength, while carbonation in a loose state led to strength reduction. SEM analysis revealed a denser microstructure in carbonated samples due to calcium carbonate and silica gel formation. Compared to traditional cement solidification, FS-C type solidified soil reduces cement consumption by 15%, decreases CO2 emissions by 299.25 g/m(3), and sequesters 85 g/m(3) of CO2. These findings highlight the potential of FS-C type solidified soil as an environmentally friendly alternative for construction applications.
Background Bermudagrass (Cynodon dactylon) has a long history as an excellent forage grass, and salt stress will inhibit its growth and development. In order to minimize the damage, it is necessary to continuously develop innovative technologies and management strategies. Results This study evaluated the salt tolerance of new Bermudagrass strains 'FB2019R101' and 'FB2019R105' compared to commercial varieties 'Wrangler' and 'A12359' under simulated soil salinity conditions through seawater irrigation. Through correlation analysis of growth, physiological, and nutritional indicators, and principal component analysis, core indicators and weights for salt tolerance evaluation were identified. The salt-tolerant varieties were 'FB2019R101' and 'FB2019R105'. Under salinity stress, the plants of Bermudagrass varieties with salt tolerance suffered less damage as a whole, which could better regulate the osmotic balance inside and outside cells, accumulate more nutrients and have stronger ability to resist salt damage. The expression level of salt-tolerant variety CdCINV1, CdSPS1, CdSUS5, and CdSWEET6 was up-regulated under salt stress. CdCINV1, CdSPS1, CdSUS5 can promote the transformation of sucrose into glucose and fructose in Bermudagrass under salt stress, and CdSWEET6 can promote the accumulation of fructose. Conclusions 'FB2019R101' and 'FB2019R105' exhibited higher salt tolerance, with minimal impact on their biomass, physiological, and nutritional indicators under salt stress. The comprehensive evaluation revealed a salt tolerance ranking of 'FB2019R105' > 'FB2019R101' > 'Wrangler' > 'A12359'. This study provides significant reference for the bioremediation of coastal saline soils and promotes research on the application of Bermudagrass under salt stress conditions. CdCINV1, CdSPS1, CdSUS5, and CdSWEET6 can improve the salt tolerance of plants by regulating the changes of carbohydrates.
Open-pit mining seriously damages the original vegetation community and soil layer and disturbs the carbon cycle of vegetation and soil, causing instability in the mining ecosystem and decrease in the carbon sequestration capacity of the mining area. With the deepening of environmental awareness and the influence of related policies, the ecological restoration of open-pit mines has been promoted. The mining ecosystem is distinct owing to the disperse distribution of mines and small scale of single mines. However, the carbon sequestration capability of mines after ecological restoration has not been clearly evaluated. Therefore, this study evaluated the carbon sequestration capacity of restoration mines, taking the mines of the Yangtze River Basin in Jurong City, Jiangsu Province as the research objects. Firstly, the visual effects of the vegetation and soil in their current status were determined through field investigation, the methods for sampling and data collection for the vegetation and soil were selected, and the specific laboratory tests such as the vegetation carbon content and soil organic carbon were clarified. Meanwhile, the evaluation system consisting of three aspects and nine evaluation indexes was established by using the analytic hierarchy process (AHP) and fuzzy comprehensive evaluation (FCE). The process of evaluation included the following: the establishment of the judgment matrix, calculation of the index weight, determination of the membership function, and establishment of the fuzzy membership matrix. Finally, the evaluation results of the restoration mines were determined with the 'excellent, good, normal and poor' grade classification according to the evaluation standards for each index proposed considering the data of the field investigation and laboratory tests. The results indicated that (1) the evaluation results of the mines' carbon sequestration capacity were of excellent and good grade at a proportion of 62.5% and 37.5%, which was in line with the field investigation results and demonstrated the carbon sequestration capacity of all the restored mines was effectively improved; and (2) the weights of the criterion layer were ranked as system stability > vegetation > soil with the largest value of 0.547, indicating the stability of the system is the main factor in the carbon sequestration capacity of the mines and the sustainability of the vegetation community and the stability of soil fixation on the slope. The proposed evaluation system effectively evaluates the short-term carbon sequestration capability of the restoration mining system according to the visual effects and the laboratory testing results, objectively reflecting the carbon sequestration capacity via qualitative assessment and quantitative analysis. The evaluation method is relatively applicable and reliable for restoration mines and can provide a reference for similar ecological restoration engineering.
Soil salinization significantly impacts the ecological environment and agricultural production, posing a threat to plant growth. Currently, there are over 400 varieties of Bougainvillea with horticultural value internationally. However, research on the differences in salt tolerance among Bougainvillea varieties is still insufficient. Therefore, this study aims to investigate the physiological responses and tolerance differences of various Bougainvillea varieties under different concentrations of salt stress, reveal the effects of salt stress on their growth and physiology, and study the adaptation mechanisms of these varieties related to salt stress. The experimental materials consisted of five varieties of Bougainvillea. Based on the actual salinity concentrations in natural saline-alkali soils, we used a pot-controlled salt method for the experiment, with four treatment concentrations set: 0.0% (w/v) (CK), 0.2% (w/v), 0.4% (w/v), and 0.6% (w/v). After the Bougainvillea plants grew stably, salt stress was applied and the growth, physiology, and salt tolerance of the one-year-old plants were systematically measured and assessed. The key findings were as follows: Salt stress inhibited the growth and biomass of the five varieties of Bougainvillea; the 'Dayezi' variety showed severe salt damage, while the 'Shuihong' variety exhibited minimal response. As the salt concentration and duration of salt stress increase, the trends of the changes in antioxidant enzyme activity and osmotic regulation systems in the leaves of the five Bougainvillea species differ. Membrane permeability and the production of membrane oxidative products showed an upward trend with stress severity. The salt tolerance of the five varieties of Bougainvillea was comprehensively evaluated through principal component analysis. It was found that the 'Shuihong' variety exhibited the highest salt tolerance, followed by the 'Lvyehuanghua', 'Xiaoyezi', 'Tazi', and 'Dayezi' varieties. Therefore, Bougainvillea 'Shuihong', 'Lvyehuanghua', and 'Xiaoyezi' are recommended for extensive cultivation in saline-alkali areas. The investigation focuses primarily on how Bougainvillea varieties respond to salt stress from the perspectives of growth and physiological levels. Future research could explore the molecular mechanisms behind the responses to and tolerance of different Bougainvillea varieties as to salt stress, providing a more comprehensive understanding and basis for practical applications.
Planting macadamia in karst rocky desertification areas has significant ecological and economic benefits. However, the production of macadamia in karst area are facing serious drought challenges due to the poor water holding capacity of soil, and less and uneven precipitation resulting with the rising of temperature in the world. Selecting and breeding drought-resistant rootstocks is one of the main measures to solve this problem in macadamia nut production in karst area. In this study, the morphological and physiological performance of half-sib family offspring of 14 macadamia cultivars were investigated under severe drought conditions and their drought resistance was evaluated comprehensively in order to select the most suitable macadamia rootstocks. Results revealed that the leaves of all rootstocks were damaged after drought stress. Among the tested rootstocks, the leaves of QA5 were damaged the most severely, and the leaves of the whole plants were chlorotic, followed by C11, A4, and NY2, while the symptoms in leaves of O.C and H2 was less obvious after drought stress for 30 d. At the same time, the O.C and H2 had the lowest drought injury index (DII), which was 0.40, while the QA5 had the highest DII value (0.82). The physiological indices, including malonaldehyde (MDA), superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), soluble protein (SP), soluble sugar (SS), proline (Pro) and relative electrolyte leakage (REL) increased (Drought tolerance coefficient, DTC > 1) after drought stress, while relative water content of leaves (L-RWC), chlorophyll (Chl), relative water content of roots (R-RWC) and root activity (RA) decreased (DTC < 1) in all cultivars. Except SP, SS and Pro, there were significant differences in other indexes among different rootstocks. The 13 physiological indices were transformed into seven independent, comprehensive indices through principal component analysis (PCA), whose cumulative contribution ratio reached 82.905%. The 14 rootstocks were divided into 3 categories based on the comprehensive evaluation value (D). The O.C and H2 were clustered into the drought-tolerant group, whose D values were 0.743 and 0.669, respectively. The NY3, 333, 788, NY116, NY1, NY2 were belonged to the intermediate drought tolerance group, the D values were 0.530, 0.508, 0.497, 0.465, 0.457 and 0.441 respectively. Lastly, A16, A4, C11. GR1, 695 and QA5 were clustered into low drought tolerance group with the lowest D values of 0.396, 0.391, 0.360, 0.360, 0.355, 0.324, respectively. The result of stepwise regression analysis showed that MDA, POD, APX, SS, REL, R-RWC and RA were the most significant physiological and biochemical indices that played a significant role in drought tolerance, and could be used as the primary evaluation and identification indicators of drought tolerance of macadamia. These results indicated that the drought-resistant rootstocks of macadamia could improve their drought resistance by stimulating the cluster root development, increasing antioxidant enzyme activity and raising the content of osmotic regulatory substances under drought stress. Our study not only provided alternative rootstocks for macadamia drought-resistant cultivation, but also provided the theoretical basis for the drought-resistant rootstock breeding of macadamia by expounding the physiological mechanism of drought-resistance.