Most natural soils exhibit a certain degree of soil structure which, in general, leads to increased strength and stiffness properties. However, the mechanical characterization of these soils based on conventional laboratory testing proves difficult in many cases due to sample disturbance. The present work aims to characterize the microstructure of a postglacial, normally consolidated, fine-grained deposit in Seekirchen, Austria, adopting in situ testing, laboratory testing on high-quality samples, and numerical analysis. The latter involves recalculating in situ piezocone penetration tests (CPTu) using an advanced constitutive model for structured soil. In contrast to existing in situ interpretation methods, the results of the numerical study, the mineralogical and hydrochemical testing, as well as the oedometer and bender element testing on undisturbed and reconstituted samples suggest that the soil is characterized by a significant amount of structure. It is demonstrated that the difference in shear wave velocity measured in situ and through bender element testing on reconstituted samples can be used as an indicator for soil structure. Ignoring the effects of structure may lead to inaccurate parameter determination for advanced constitutive models, which are subsequently employed to solve complex boundary value problems in geotechnical practice. As a consequence, the prediction of expected displacement may not be reliable.