The moons of Jupiter and Saturn, such as Europa and Enceladus, are strong candidates for the search for life outside of Earth. Together with the use of direct observational methods, physical and chemical processes that take place on icy moons may be studied on planetary field analogs, that is, on similar reachable locations on Earth. Fieldwork performed on planetary field analogs can test protocols and technology that may be applied on future space missions to extraterrestrial environments. The Arctic is a strong candidate for such studies. This study assesses a spectroscopic protocol for biosignature detection in the Arctic, as a proxy to icy moons. Samples of ice and the water underneath were collected by our team in different locations at and nearby Hudson Bay, Canada, and spectroscopic analysis detected the presence of humic acid in all the samples. On the contrary, biosignatures such as amino acids and beta-carotene may have been present in concentrations below the limit of detection of the equipment used. With proper optimization, it will be possible to implement this simple protocol that relies on lightweight equipment in future space missions to icy moons.

RISTRETTO is a visible high-resolution spectrograph fed by an extreme adaptive optics (AO) system, to be proposed as a visitor instrument on ESO VLT. The main science goal of RISTRETTO is to pioneer the detection and atmospheric characterisation of exoplanets in reflected light, in particular the temperate rocky planet Proxima b. RISTRETTO will be able to measure albedos and detect atmospheric features in a number of exoplanets orbiting nearby stars for the first time. It will do so by combining a high-contrast AO system working at the diffraction limit of the telescope to a high-resolution spectrograph, via a 7-spaxel integral-field unit (IFU) feeding single-mode fibers. Further science cases for RISTRETTO include the study of accreting protoplanets such as PDS70b/c through spectrally-resolved H-alpha emission, and spatially-resolved studies of Solar System objects such as icy moons and the ice giants Uranus and Neptune. The project is in the manufacturing phase for the spectrograph sub-system, and the preliminary design phase for the AO front-end. Specific developments for RISTRETTO include a novel coronagraphic IFU combining a phase-induced amplitude apodizer (PIAA) to a 3D-printed microlens array feeding a bundle of single-mode fibers. It also features an XAO system with a dual wavefront sensor aiming at high robustness and sensitivity, including to pupil fragmentation. RISTRETTO is a pathfinder instrument in view of similar developments at the ELT, in particular the SCAO-IFU mode of ELT-ANDES and the future ELT-PCS instrument.

Growing evidence of the potential habitability of Ocean Worlds across our solar system is motivating the advancement of technologies capable of detecting life as we know it-sharing a common ancestry or physicochemical origin with life on Earth-or don't know it, representing a distinct emergence of life different than our one known example. Here, we propose the Electronic Life-detection Instrument for Enceladus/Europa (ELIE), a solid-state single-molecule instrument payload that aims to search for life based on the detection of amino acids and informational polymers (IPs) at the parts per billion to trillion level. As a first proof-of-principle in a laboratory environment, we demonstrate the single-molecule detection of the amino acid L-proline at a 10 mu M concentration in a compact system. Based on ELIE's solid-state quantum electronic tunneling sensing mechanism, we further propose the quantum property of the HOMO-LUMO gap (energy difference between a molecule's highest energy-occupied molecular orbital and lowest energy-unoccupied molecular orbital) as a novel metric to assess amino acid complexity. Finally, we assess the potential of ELIE to discriminate between abiotically and biotically derived alpha-amino acid abundance distributions to reduce the false positive risk for life detection. Nanogap technology can also be applied to the detection of nucleobases and short sequences of IPs such as, but not limited to, RNA and DNA. Future missions may utilize ELIE to target preserved biosignatures on the surface of Mars, extant life in its deep subsurface, or life or its biosignatures in a plume, surface, or subsurface of ice moons such as Enceladus or Europa. One-Sentence Summary: A solid-state nanogap can determine the abundance distribution of amino acids, detect nucleic acids, and shows potential for detecting life as we know it and life as we don't know it.

Saturn's Moon Titan receives volatiles into the top of its atmosphere-including atomic oxygen-sourced from cryovolcanoes on Enceladus. Similar types of atmosphere exchange from one body to another, such as O-2 and O-3 sourced from TRAPPIST-1 d, could be introduced into the upper atmosphere of TRAPPIST-1 e and might be interpreted as biosignatures. We simulate this potential false-positive for life on TRAPPIST-1 e, by applying an external influx of water and oxygen into the top of the atmosphere using a coupled 1-D photochemical-climate model (Atmos), to predict atmospheric composition. In addition, synthetic spectral observations are produced using the Planetary Spectrum Generator for the James Webb Space Telescope, Origins Space Telescope, Habitable Exoplanet Observatory and Large Ultra-violet/Optical/Infrared Surveyor to test the detectability of abiotic-generated O-2 and O-3 in the presence of abiotic and biotic surface fluxes of CH4. We determine that the incoming flux of material needed to trigger detection of abiotic O-2/O-3 by any of these observatories is more than two orders of magnitude (1 x 10(12) molecules/cm(2)/s) above what is physically plausible.

RISTRETTO is a visible high-resolution spectrograph fed by an extreme adaptive optics (XAO) system, to be proposed as a visitor instrument on ESO VLT. The main science goal of RISTRETTO is the detection and atmospheric characterization of exoplanets in reflected light, in particular the temperate rocky planet Proxima b. RISTRETTO will be able to measure albedos and detect atmospheric features in a number of exoplanets orbiting nearby stars for the first time. It will do so by combining a high-contrast AO system working at the diffraction limit of the telescope to a high-resolution spectrograph, via a 7-spaxel integral-field unit (IFU) feeding single-mode fibers. Further science cases for RISTRETTO include the study of accreting protoplanets such as PDS 70 b & c through spectrally-resolved Hff emission; and spatially-resolved studies of Solar System objects such as icy moons and the ice giants Uranus and Neptune. The project is in an advanced design phase for the spectrograph and IFU/fiber-link sub-systems, and a preliminary design phase for the AO front-end. Construction of the spectrograph and IFU/fiber-link will start at the end of 2022. RISTRETTO is a pathfinder instrument in view of similar developments at ESO ELT, in particular the SCAO-IFU mode of ELT-ANDES and the future ELT-PCS instrument.

In this chapter, we review the contribution of space missions to the determination of the elemental and isotopic composition of Earth, Moon and the terrestrial planets, with special emphasis on currently planned and future missions. We show how these missions are going to significantly contribute to, or sometimes revolutionise, our understanding of planetary evolution, from formation to the possible emergence of life. We start with the Earth, which is a unique habitable body with actual life, and that is strongly related to its atmosphere. The new wave of missions to the Moon is then reviewed, which are going to study its formation history, the structure and dynamics of its tenuous exosphere and the interaction of the Moon's surface and exosphere with the different sources of plasma and radiation of its environment, including the solar wind and the escaping Earth's upper atmosphere. Missions to study the noble gas atmospheres of the terrestrial planets, Venus and Mars, are then examined. These missions are expected to trace the evolutionary paths of these two noble gas atmospheres, with a special emphasis on understanding the effect of atmospheric escape on the fate of water. Future missions to these planets will be key to help us establishing a comparative view of the evolution of climates and habitability at Earth, Venus and Mars, one of the most important and challenging open questions of planetary science. Finally, as the detection and characterisation of exoplanets is currently revolutionising the scope of planetary science, we review the missions aiming to characterise the internal structure and the atmospheres of these exoplanets.

The quest for life on other planets is closely connected with the search for water in liquid state. Recent discoveries of deep oceans on icy moons like Europa and Enceladus have spurred an intensive discussion about how these waters can be accessed. The challenge of this endeavor lies in the unforeseeable requirements on instrumental characteristics both with respect to the scientific and technical methods. The TRIPLE/nanoAUV initiative is aiming at developing a mission concept for exploring exo-oceans and demonstrating the achievements in an earth-analogue context, exploring the ocean under the ice shield of Antarctica and lakes like Dome-C on the Antarctic continent.