Preparation and characterization of biopolymer-based packaging materials have significantly gained importance because of sustainability, biodegradability, and eco-friendly nature. In this study, novel wheat gluten (WG)/cloisite 30B (C30B) organoclay-based bionanocomposite (BNC) films were prepared by solution casting method at various C30B concentrations (5%, 10%, and 15%). X-ray diffraction and field emission scanning electron microscopy revealed intercalation/exfoliation of C30B sheets into the WG matrix. WG-C30B 10% film was thermostable. It showed low surface roughness along with higher water barrier properties and surface hydrophobicity. The tensile strength values of WG and WG-C30B 10% films were found to be 0.7 +/- 0.02 and 1.11 +/- 0.01, respectively, indicating improvement in mechanical properties. WG-C30B 10% film demonstrated antibacterial activity against both Staphylococcus aureus and Salmonella enterica. Shelf life of green grapes was monitored under different conditions: 4 degrees C, ambient conditions, and 42 degrees C. WG-C30B 10% film proved effective in extending shelf life up to 18 days under ambient conditions. More than 50% of the bionanocomposite films were degraded in agricultural soil within 2 weeks, while completely degraded in sewage sludge soil after a few days. WG-C30B 10% film appeared to be promising regarding the demonstrated physico-chemical and antibacterial properties. This report would be useful in preparing biodegradable biopolymer-based packaging materials.