The microbial-induced calcite precipitation (MICP) technique has been developed as a sustainable methodology for the improvement of the engineering characteristics of sandy soils. However, the efficiency of MICP-treated sand has not been well established in the literature considering cyclic loading under undrained conditions. Furthermore, the efficacy of different bacterial strains in enhancing the cyclic properties of MICP-treated sand has not been sufficiently documented. Moreover, the effect of wetting-drying (WD) cycles on the cyclic characteristics of MICP-treated sand is not readily available, which may contribute to the limited adoption of MICP treatment in field applications. In this study, strain-controlled consolidated undrained (CU) cyclic triaxial testing was conducted to evaluate the effects of MICP treatment on standard Ennore sand from India with two bacterial strains: Sporosarcina pasteurii and Bacillus subtilis. The treatment durations of 7 d and 14 d were considered, with an interval of 12 h between treatments. The cyclic characteristics, such as the shear modulus and damping ratio, of the MICP-treated sand with the different bacterial strains have been estimated and compared. Furthermore, the effect of WD cycles on the cyclic characteristics of MICP-treated sand has been evaluated considering 5-15 cycles and aging of samples up to three months. The findings of this study may be helpful in assessing the cyclic characteristics of MICP-treated sand, considering the influence of different bacterial strains, treatment duration, and WD cycles. (c) 2025 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).

Microbially Induced Calcite Precipitation (MICP) is an eco-friendly method for improving sandy soils, relying on micro-organisms that require nitrogen and essential nutrients to induce carbonate mineral precipitation. Given the substantial annual generation of chicken manure (CM) and the associated challenges in its disposal resulting in environmental pollution, the nutrient-rich composted form of this waste material is proposed in this study as a supplementary additive (along with more costly industrial reagents, e.g., urea) to provide the necessary carbon and nitrogen for the MICP process. To this end, different CM contents (5 %, 10 %, and 15 %) along with various concentrations of cementation solution (1 M, 1.5 M, and 2 M) are employed in multiple improvement cycles to augment the efficiency of the MICP technique. Unconfined Compressive Strength (UCS), Ultrasonic Pulse Velocity (UPV), and Water Absorption (WA) tests are performed to assess the mechanical properties of the samples before and after exposure to freeze-thaw (F-T) cycles, while SEM, XRD, and FTIR analyses are carried out to delineate the formation of calcite within the porous structure of MICP-CM-treated sands. The findings suggest that an optimum percentage of CM (10 %) in the MICP process not only contributes to environmental conservation but also significantly enhances all the mechanical properties of bio-cemented sandy soils due to markedly improved bonding within their porous fabric. The results also show that although prolonged exposure to consecutive F-T cycles causes a reduction in strength and stiffness of enhanced MICP-treated soils, the mechanical properties of such geo-composites still remain within an acceptable range for optimal CM-enhanced biocemented mixtures, significantly superior to those of MICP-treated sands.