Particle segregation is a widespread phenomenon in nature. Vertical vibration systems have been a focal point in studying particle segregation, providing valuable insights into the mechanisms and patterns that influence this process. However, despite extensive research on the mechanisms and patterns of particle separation, the consequences, particularly the mechanical properties of samples resulting from particle segregation, remain less understood. This study aims to investigate the segregation process of a binary mixture under vertical vibration and examine the consequences through monotonic and cyclic triaxial drained tests. The results reveal that large and small particles segregate nearly simultaneously, with more thorough separation observed for large particles. The segregation index, Ds, effectively describes this evolution process, offering a quantitative metric for both mixing and segregation. Granular temperature analysis unveils three distinct states during segregation: solid-like, fluid-like, and solid-liquid transitional phase, corresponding to varying activity levels of particle segregation. Drained triaxial shear tests demonstrate the sensitivity of stress-strain relationships to the degree of segregation. Interestingly, ultimate strength is found to be essentially unrelated to the degree of segregation. When the segregation index approaches zero, signifying particles approaching a uniform distribution, the granular system reaches a harmonic state. This state exhibits optimal mechanical performance characterised by maximum peak stress, friction angle, and the highest elastic modulus. These findings underscore the potential impact of segregation on the mechanical response of granular mixtures and emphasise the necessity of a comprehensive understanding of particle segregation in soil mechanics.

The proportional strain loading test is a prevalent method for investigation diffuse instability. The majority of current research concentrates on narrowly graded materials, with relatively less focus on binary mixtures under proportional strain loading. Therefore, a series of numerical tests have been conducted using the discrete element method to study the influence of fine content and strain increment ratio on the binary mixtures. The test results show that the fine content of binary mixtures is intimately connected to the critical strain increment ratio which precipitate a transition from stability to instability. Binary mixtures characterized by a low stress ratio at the onset of instability also demonstrate a heightened sensitivity to shifts in strain increment ratio. The macroscopic responses, such as the stress ratio at the onset of instability, shear strength, and pore water pressure, exhibit different trends of variation with the fine content compared to microscopic responses, including coordination number, friction mobilization index, and the proportion of sliding contacts. Furthermore, the anisotropy coefficient is introduced to dissect the sources of anisotropy at onset of instability, revealing that strong contact fabric anisotropy can mirror the evolution of the stress ratio. The stress ratio at onset of instability is predominantly influenced by anisotropy in contact normal and normal contact force.

Loose granular materials may also exhibit instability behaviors similar to liquefaction under drained conditions, commonly referred to as diffuse instability, which can be studied through constant shear drained (CSD) tests. So far, the research on CSD in binary mixtures is still insufficient. Therefore, a series of numerical tests using the discrete element method (DEM) were conducted on binary mixtures under CSD path. The possible model of instability is categorized into type I and type II, type I instability occurs prior to reaching the critical state line (CSL), whereas type II instability occurs after exceeding the CSL. The study analyzes the macroscopic instability behavior and the impact of fine content (FC) on macroscopic instability behavior. The numerical results show that as FC increases, the slope of the instability line (IL) increases initially and then falls in the p-q plane. In the e-p plane, the IL decreases initially and then ascends. The instability type of the binary mixtures is influenced not only by relative density but also by FC. The stability index increased first and then decreased with the increase of FC. The microscopic origin of binary mixtures instability is explored by investigating the fabric-stress relationship. The collapse of the weak contact sub-network triggers the specimen instability, while the strong contact sub-network dictates the difficulty of achieving instability. FC influences the evolution of fabric anisotropy of the strong and weak contact networks, thereby controlling the macroscopic instability behavior of binary mixtures.

There is growing concern that sprayed neonicotinoid pesticides (neonics) persist in mixed forms in the environmental soil and water systems, and these concerns stem from reports of increase in both the detection frequency and concentration of these pollutants. To confirm the toxic effects of neonics, we conducted toxicity tests on two neonics, clothianidin (CLO) and imidacloprid (IMD), in embryos of zebrafish. Toxicity tests were performed with two different types of mixtures: potential mixture compounds and realistic mixture compounds. Potential mixtures of CLO and IMD exhibited synergistic effects, in a dose-dependent manner, in zebrafish embryonic toxicity. Realistic mixture toxicity tests that are reflecting the toxic effects of mixture in the aquatic environment were conducted with zebrafish embryos. The toxicity of the CLO and IMD mixture at environmentally-relevant concentrations was confirmed by the alteration of the transcriptional levels of target genes, such as cell damage linked to oxidative stress response and thyroid hormone synthesis related to zebrafish embryonic development. Consequently, the findings of this study can be considered a strategy for examining mixture toxicity in the range of detected environmental concentrations. In particular, our results will be useful in explaining the mode of toxic action of chemical mixtures following short-term exposure. Finally, the toxicity information of CLO and IMD mixtures will be applied for the agricultural environment, as a part of chemical regulation guideline for the use and production of pesticides.

The fabric structure and dynamic behaviour of granular materials have been extensively studied in geotechnical engineering due to their considerable impact on permeability and mechanical properties. However, particle segregation, causing significant structural changes, remains inadequately understood, especially concerning its dynamic evolution in both global and local segregation processes. This study aims to investigate the motion of particles and evolution of internal microstructure in binary mixtures under vibrational conditions. The emphasis lies in comprehending both global and local time segregation processes, along with elucidating the potential underlying mechanisms. Through DEM simulations, it is observed that large particles tend to rise to the surface of the container while small particles aggregate at the bottom, resulting in the well-known Brazil Nut Effect. As the vibration intensity increases, the degree of segregation becomes more pronounced. Vertical segregation precedes radial segregation and eventually leads to stable separation of the binary mixture. To comprehensively analyse the segregation behaviour, we introduce a segregation index and reveal a correlation between vertical and radial segregation. Additionally, the ascending process exhibits characteristics similar to compressed solid blocks, while the descending process resembles fluid-like behaviour, suggesting a phase transition phenomenon during particle segregation. The study further highlights the role of pore filling and convective rolling as driving mechanisms for particle segregation. These findings emphasize the potential impact of external disturbances on the microstructure of granular mixtures, with implications for scenarios such as earthquakes, debris flows, and traffic loads.

One of the critical steps in the root crop harvesting process is screening tubers from soil. However, low screening efficiency seriously hinders the rapid development of the root crop industry. Clarifying the tuber-soil mixture separation behaviour and establishing the connection between vibration, airflow parameters, and separation index (SI) is critical to increasing screening efficiency. Corydalis Yanhusuo is employed as the research object, and the three-dimensional scale distribution and mechanical properties of tubers and soil particles are first counted. Then, a vibration and airflow coupling separation model of the tuber-soil mixture was constructed using the computational fluid dynamics and discrete element method (CFD-DEM) coupling method, and the physical parameters in the model were calibrated. A new method for calculating the SI is proposed. The relationship between vibration amplitude, frequency, airflow velocity, SI, and separation velocity was analysed. Simultaneously, the porosity change in the particle group during the separation process was investigated, and the relationship between vibration, frequency, and airflow velocity on the separation dynamics of binary mixtures was revealed by utilising data visualisation and frequency domain analysis. The platform for the vibration and airflow separation physical test was built. The separation behaviour of mixed particles in various parameters was discussed, as was the feasibility and accuracy of the numerical simulation results. The results of this study can provide theoretical support for the efficient screening of tuber-soil mixtures and further promote the rapid development of the root industry.

Driven by external compressions or shears, the granular material composed of non-convex shapes often undergoes self-assembly and becomes entangled, resulting in denser packings. In this work, the biaxial compression of monophasic and binary mixture composed of 2D intersecting crosses have been simulated via the discrete element method. As the aspect ratio increases, both the yield strength and elastic modulus initially increase before reaching a peak at w = 0.5. In binary mixtures, an antagonistic effect has been observed in mechanical properties of granular material. The decrease in strength and stiffness in binary mixtures can be attributed to the constraints imposed by multi-point contacts, along with the local order degree, which is particularly unique for non-convex particle packings. Building upon this understanding, we have extended the empirical formula into predicting the mechanical behavior of non-convex binary mixtures. This extension incorporates an additional non-linear term determined by both the shape factor and component fraction.