In order to accurately model the machine-lunar soil simulant interaction, this study combined physical and simulation experiments to calibrate the discrete element simulation parameters of the JLU-H lunar highland simulant. First, the intrinsic parameters and the true angle of repose of the JLU-H were determined through physical tests to provide data for subsequent simulation tests. A Plackett-Burman test was designed to identify and select the parameters that have a significant effect on the angle of repose. The range of values of the significant parameters was then optimized using the steepest climb test. The Box-Behnken test was then utilized to calibrate and obtain the optimal parameter combinations. Finally, a validation test of the angle of repose was conducted using the calibrated DEM parameters. The relative error between the simulation results and the test results was 1.54 %. Then further straight shear tests were conducted to verify the accuracy and validity of the DEM parameters. The results show that the calibrated parameters can provide a reference for the selection of discrete element simulation parameters for lunar soil simulant and the design and optimization of drilling and excavation machinery for lunar exploration. (c) 2024 COSPAR. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.