The use of chemical pesticides in agriculture leads to the accumulation of harmful compounds in soil and plants that can cause diseases of humans and animals. The biological method of plant protection is a promising alternative to chemical pesticides. The purpose of this study was to analyze the antagonistic activity of the Acinetobacter sp. GET13 strain against common bacterial and fungal pathogens of plant diseases in in vitro and in planta experiments. As a result, the effect of the bacterium on the growth of phytopathogenic bacteria (Clavibacter michiganensis, Erwinia carotovora, Pectobacterium carotovorum and Pseudomonas syringae), as well as phytopathogenic fungi (Helminthosporium sativum, Piricularia oryzae.) that cause serious damage to agriculture, was studied. To confirm the results obtained in these experiments, an in planta experiment was conducted on Phaseolus vulgaris (L.) The effectiveness of Acinetobacter GET13 strain for plant protection against phytopathogens was proved based on the results of taking into account the linear function between weight and volume parameters of plants at the end of the experiment. Therefore, this strain has the potential to create a biological product.

The application of microorganisms to improve the mechanical properties of soil is a new developing research area. A new native bacteria extracted from soil was introduced for the biological improvement of soil geotechnical parameters. The isolate was identified as Acinetobacter calcoaceticus S1. Sporosarcina pasteurii was used as a positive control. Direct shear tests were performed on the nontreated soil and soils treated with bacteria to determine the shear strength, adhesion and angle of internal friction. The treatment period was 40 days. The shear wave velocity was measured.The results showed that the untreated sample had relatively constant shear strength, but the shear strength of the treated soils increased significantly. The soil treated with A. calcoaceticus had greater shear strength. The angle of internal friction increased for the treated soils with A. calcoaceticus (39.3%) and S. pasteurii (28.6%). The greatest cohesion was found for soil treated with A. calcoaceticus, reaching 0.66 and 0.56 kg/cm2 for S. pasteurii. The shear wave velocity in the treated soils increased significantly. The results confirmed the ability of native A. calcoaceticus to improve soil geotechnical parameters. Calcium carbonate precipitation fills the voids between soil particles and forms a gel, which makes effective connections between soil particles and makes them coalesce and grow larger.