The foundation soil below the structure usually bears the combined action of initial static and cyclic shear loading. This experimental investigation focused on the cyclic properties of saturated soft clay in the initial static shear stress state. A range of constant volume cyclic simple shear tests were performed on Shanghai soft clay at different initial static shear stress ratios (SSR) and cyclic shear stress ratios (CSR). The cyclic behavior of soft clay with SSR was compared with that without SSR. An empirical model for predicting cyclic strength of soft clay under various SSR and CSR combinations was proposed and validated. Research results indicated that an increase of shear loading level, including SSR and CSR, results in a larger magnitude of shear strain. The response of pore water pressure is simultaneously dominated by the amplitude and the duration of shear loading. The maximum pore water pressure induced by smaller loading over a long duration may be greater than that under larger loading over a short duration. The initial static shear stress does not necessarily have a negative impact on cyclic strength. At least, compared to cases without SSR, the low-level SSR can improve the deformation resistance of soft clay under the cyclic loading. For the higher SSR level, the cyclic strength decreases with the increase of SSR.