Allolobophora caliginosa, , an earthworm, was exposed to caffeine (CAF) via artificial soil to evaluate the effects on antioxidant enzymes in animals treated to 0, 10, 20, 40, and 80 mg CAF/kg soil after 7, 14, 28, and 56 d of exposure. There is evidence that antioxidant enzymes protect cells from free radical damage. A high CAF concentration generated changes in the activities of superoxide dismutase (SOD), catalase (CAT), and guaiacol peroxide (POD), but had slight effects on malondialdehyde (MDA) levels after 56 d of exposure. Earthworms' ' MDA levels elevated somewhat after 7, 14, and 28 d. Earthworms treated with CAF were unable to induce the cytotoxic action over a very long period of time (56 d), as three enzymes [polyphenol oxidase (PPO), acetyl cholinesterase (AChE), and cellulose] were significantly inhibited. These data support the notion that oxidative stress plays a role in the response of earthworms to CAF poisoning.

来源平台：ISJ-INVERTEBRATE SURVIVAL JOURNAL