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In this study, a novel approach, double enzyme-enhanced microbial-induced carbonate precipitation (E-MICP), was proposed to enhance the bioconsolidation efficiency of sandy soil. The results demonstrated that, compared to traditional bioconjugation techniques, the soil bioconsolidation rate, unconfined compressive strength (UCS), and CaCO3 content increased by 5.89, 2.76, and 2.1 times, respectively, following E-MICP treatment. Microscopic characterization revealed that E-MICP facilitated the formation of various cementation structures, which accelerated the precipitation of amorphous calcium carbonate (ACC) and the dehydrogenation of hydrated ACC. The strong chemical bonding of Si-O-Ca and intermolecular hydrogen bonding formed at the interface between sand particles and CaCO3 were identified as key factors contributing to the enhanced bioconjugation performance of E-MICP. This novel E-MICP process overcomes the limitations of traditional bioconjugation methods. The findings of this study offer a theoretical foundation and innovative perspectives for the ecological management of sandy soil.

来源平台：JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING