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The overapplication of chemical pesticides will cause heavy pollution in water, soil, and foodstuff, and cause irreversible damage to the ecological environment and human health. Therefore, it is imperative to develop a highly sensitive and reliable tool for detecting pesticide residues in the environment. In this work, a novel nopinone-based fluorescent probe THIP-OCP for the detection of parathion-methyl was constructed from BchE inhibition principles. The ester bond in THIP-OCP was hydrolyzed by BchE, leading to the release of the fluorophore THIP-OH and a significant enhancement of the fluorescence signal at 547 nm. However, parathion- methyl could inhibit BchE activity significantly and resulted in fluorescence quenching at 547 nm. Probe THIP-OCP was effectively used to detect BchE and parathion-methyl, and the detection limits were as low as 8.56 U/L and 0.79 mu g/mL, respectively. A portable smartphone-based analysis platform for quantitative and qualitative analysis of parathion-methyl in soil was developed from probe THIP-OCP. This probe can also be used to detect butyrylcholinesterase (BchE) and parathion-methyl in living cells and zebrafish, providing a new tool for monitoring BchE and parathion-methyl in living systems, which is helpful for protecting human life and health. Therefore, the probe THIP-OCP is regarded as a promising tool for monitoring environmental safety and biological health systems.

来源平台：JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING