The Tuotuohe River basin, which locates at the source region of Yangtze River, is selected as the study area in this project. Based on observation, stable isotope and remote sensing technology, runoff generation mechanism at the glaciers, frozen soil and snow cover region is analyzed. The contribution of glacier melting runoff to total runoff at basin scale is quantified by stable isotope runoff seperation method. The mechanism for impacts of air temperature and glacier area on glacier melting runoff is analyzed based on air temperature and glacier area datasets obtained from observation and remote sensing interpretation. Based on observed soil temperature, soil water content and field water capacity datasets, the process of frozen soil frozing and thawing is analyzed. Besides that, the releasing water from frozen soil is calculated for analysing their effects on runoff. The spatial distribution of snow cover at the study area is analyzed by remote sensing snow cover products data. Considering observed precipitation, air temperature and soil temperature in the same periods,snow water equivalent is estimated for their effects on runoff. The spatial and temperal distributions of glacier, frozen soil and snow cover at the Tuotuohe River basin are analyzed, and based on the Degree-day factor model, runoff generation mechanisms affect by glacier, frozen soil and snow cover at basin scale are analyzed. This study could contribute to the study of understanding the regional hydrology processes at glaciers and frozen soil effect region, and also be meaningful to the theory research of the basin water cycle under the changing environments.