新型分子印迹整体柱特异性分离提取新疆药材中植物多酚及活性单体的研究

分子印迹聚合物 提取分离 植物多酚 新疆特色药材 整体柱
刘照胜 2014-01 项目
Plant polyphenols are plant secondary metabolites with the hydrophilic character. The distinctive characteristics of the phenolic O-H bond, in plant polyphenols, have been implicated in diverse functional roles to play important roles in long-term health protection. The content of plant polyphenols in Xinjiang medicinal herbs is higher due to the influence of climate and environment. The study on plant polyphenols will be benefit to develop traditional medicine with distinguishing feature in Xinjiang. Since similar chemical structure and property, it is hard and paid-cost to obtain a pure compound by isolation and is a challenge in the study of polyphenols chemistry. Molecularly imprinted polymers (MIPs) have the merit of specific selectivity to template, and monolithic column with good permeability can be used at high flow rate for separation. In view of facts above, in this study, ten MIPs monoliths with the imprinting of natural compounds, including punicalagin, will be prepared considering the matrix, polymerization approach and compositions of MIPs. The new MIPs include ZIP-like MIP, metal pivot-based MIPs in ionic liquid, MIPs based on macromolecular crowding, one-monomer MIPs and β-cyclodextrins-based MIPs. The adsorption capacity of the MIPs will be investigated and the imprinting sites are characterized by adsorptive kinetics and thermodynamics. In addition, the effect of polymerization variables on the structure and performance of the resulting MIPs monolith will be studied. Utilizing the MIPs monoliths, new approaches will be developed to selective extraction the components from medicinal herbs in Xinjiang. Moreover, new perspectives toward polyphenols chemistry will be provided.
植物多酚是水溶性植物次生代谢产物,其独特酚基可多方面保护人类健康。新疆药材因受气候环境影响,多酚含量明显较高,其研究和开发有利于新疆传统医药的特色发展。由于植物多酚分子间的化学结构和性质极其相似,分离纯化为单体非常困难且提取成本很高,是当前植物多酚化学的主要挑战。本课题利用分子印迹聚合物(MIP)对目标分子具有特异选择性,可以其为模板量身定做的特性,并结合整体柱渗透性好可高流速下进行分离的优点,首次以安石榴苷等十个单体化合物为模板,从MIP的材质、聚合方法及聚合物组成等三个方面,制备具有拉链型、金属离子枢纽型、大分子拥挤试剂协助型、功能单体与交联剂二合一型及β-环糊精型的MIP整体柱,考察这些新型MIP的选择性吸附能力,表征其吸附动力学及热力学性质,探索聚合参数对MIP整体柱结构及性能影响的规律,以此为基础发展对新疆特色药材药效组分选择性分离提纯的新方法,为植物多酚化学的研究提供新的思路。